Citations

EAE immunization and clinical evaluation:

Age-matched WT and Tph-1/mice were immunized subcutaneously with 125 µg MOG3555 peptide (Peptides International) emulsified in IFA (Sigma-Aldrich) supplemented with 0.5 mg/mouse Mycobacterium tuberculosis H37 (Difco Laboratories) on day 0 and an i.p. injection of 200 ng pertussis toxin (List Biologicals) on days 0 and 2. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Experimental Procedures 

Reagents Ultrapure LPS (Escherichia coli 0111:B4) was from List Biological Laboratories, Inc. (Vandell Way, CA) …

Animals:

… In this study, we further elucidate the pathways involved in NO-mediated TACE activation and show that LPS stimulation upregulates iNOS expression in mouse hepatocytes, which leads to TACE and iRhom2 phosphorylation in a cGMP/PKG dependent manner. …

• Product #421 – ULTRA PURE LPS from Escherichia coli O111:B4

… Quantitative RT-PCR. Peritoneal Ms were treated with or without 10 ng/ml of ultrapure E. coli LPS (0111:B4, List Biological Laboratories) for 2 h. …

• Product #421 – ULTRA PURE LPS from Escherichia coli O111:B4

Induction of EAE in Mice by Immunization with MOG and Adjuvant and Treatment with HspB5 :

EAE was induced by procedures previously described (35). Briefly, EAE was induced in female C57BL/6J mice (Jackson Laboratories, Sacramento, CA) at 9 weeks of age by subcutaneous immunization in the flank with an emulsion containing 200 µg of myelin oligodendrocyte glycoprotein(3555) (MOG3555; MEVGWYRSPFSRVVHLYRNGK) in saline and an equal volume of complete Freund’s adjuvant containing 4 µg/ml of mycobacterium tuberculosis H37RA (Difco Laboratories, Detroit, MI). All mice were administered 400 ng of pertussis toxin (List Biological, Campbell, CA) intraperitoneally at 0 and 48 h post-immunization. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Los ratones fueron sensibilizados va oral utilizando agujas intragstricas (01-2902B; Fisher Scientific) y una solucin de 1 mg de protenas de man ms 10 µg de la toxina clera (CT; List Biological Laboratories) disueltos en 10 µl de agua estril. …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

Immunohistochemistry for tracers:

… For double labeling with CTb, following BDA detection, free-floating sections were blocked 1 hr in 0.3% PBST with NRS (VECTASTAIN Elite Kit, Vector Labs). Blocked sections were incubated 2 hrs at RT in goat anti-CTb (110000 dilution, List Biological Laboratories) followed by 1 hr in rabbit anti-goat biotinylated secondary antibody (VECTASTAIN Elite Kit) and then reacted 30 min in ABC solution. DAB staining was as above, but for 3 min without nickel to give a brown reaction product. …

• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

Tissue Processing, Analysis, and Immunohistochemistry

Stellate ganglia:

… Tissue sections were washed in 10 mM Tris, 0.9% NaCl, 0.05% thimerosal in 10 mM phosphate buffer (TPBS), pH 7.4, containing 0.3% Triton X-100 for 3 10 min then incubated in 10% heat-inactivated normal horse serum (NHS, Invitrogen) in TPBS-Triton for at least 1 h. The sections were then incubated in goat anti-CTB antiserum (1:25000, List Biologicals) in TPBS-Triton containing 10% NHS for 3 days at room temperature. …

• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

Materials:

… Nicked anthrax PA 63 from B. anthracis was obtained from List Biological Laboratories Inc., Campbell, CA. …

Binding Experiments:

The binding of the His-tagged proteins to the C2II-channel and the binding component PA₆₃ was investigated with titration experiments similar to those performed previously to study the binding of 4-aminoquinolones to the C2II- and PA₆₃-channels and EF and LF to the PA₆₃-channel in single- or multi-channel experiments [38][40]. The aqueous phase contained always 150 mM KCl, buffered with 10 mM MES-KOH to pH 5.5 to pH 6. The C2II- and PA₆₃-channels were reconstituted into lipid bilayers. About 60 minutes after the addition of either activated C2II or PA₆₃ to the cis-side of the membrane, the rate of channel insertion in the membranes was very small. Then concentrated solutions of His-tagged proteins were added to the cis-side of the membranes while stirring to allow equilibration.

• Product #174 – Anthrax Protective Antigen, Activated (PA 63) from B. anthracis

Intra-ocular injections:

The B subunit of the Cholera Toxin complex (CTB) has been shown to be a highly sensitive anterograde tracer for RGCs (Angelucci et al., 1996; Reiner et al., 1996; Matteau et al., 2003), and therefore the preferred tracer for this study. Rats were first anesthetized with 25% isoflurane mixed with oxygen at a flow rate of 1l/min., using an isoflurane vaporizer (Smiths Medical, Dublin, OH, USA) While maintained at the appropriate level of anesthesia, subjects were subcutaneously injected with buprenorphine (0.06mg/kg rat weight). Subjects then received, via syringe, 56 2l injections of either unconjugated CTB in one eye, or fluorophor-conjugated CTB in both eyes.

The monocular injections were administered into the vitreous chamber of the left eye only, and comprised a 1% CTB solution (List Biological Laboratories, Inc., Campbell, CA, USA) mixed with 2% dimethyl sulfoxide (DMSO) diluted in sterile water. …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

La CTb lyophilise (… List Biological Laboratories, CA) est reconstitue dans 100µL de tampon phosphate (PB) 0,1M pH 6 dans lequel la CTb est charge positivement, permettant ainsi son injection par iontophorse.

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt