Citations

Cell culture and stimulation:

… RAW 264.7 cells expressing G_i2 and -galactosidase were stimulated with LPS (10ng/ml, ultra-pure LPS from Escherichia coli O111:B4, List Laboratories, Campbell, CA), Pam3CSK4 (1g/ml, Invivogen) or Poly (I:C) (10g/ml Invivogen) for 24 hours. LPS-induced TNF and IL-6 production were analyzed by enzyme-linked immunosorbant assay (ELISA). …

• Product #421 – ULTRA PURE LPS from Escherichia coli O111:B4

Reagents and cell culture:

LPS (R595) (List Biological Laboratories, Inc., Campbell, CA, USA), …

Flow cytometry:

… For intracellular cytokine staining, cells were incubated with LPS (100ngml¹) in the presence of GolgiStop (2M; BD Biosciences) for 5h. Surface and intracellular cytokine staining were performed as described elsewhere.²⁵ In some experiments, BM cells were stimulated with LPS (100ngml¹) or Pam3CS(K)4 (100ngml¹) for 24h in the absence or presence of anti-TNF antibody (10gml¹), and were subjected to intracellular cytokine staining.

• Product #304 – LPS from Salmonella minnesota R595 (Re)

Cell Culture:

Primary murine tracheal epithelial cells (MTECs) were isolated and cultured … Sixteen hours before treatment, cells were starved in medium containing 0.5% FBS. Cells were exposed to 1 g/ml LPS (List Biological Laboratories, Inc., Campbell, CA), …

Mice

C57Bl/6 mice were anesthetized and received 5 g LPS oropharyngeally (List Biological Laboratories, Inc.), and 24 hours later were killed by pentobarbital injection. The right lobe was flash-frozen in liquid nitrogen and pulverized, and the left lobe was inflated with 4% paraformaldehyde. Five-micron sections were used to assess the localization of RelB in situ via immunofluorescence, …

Author did not specify which List Labs LPS product was utilized in their research.  List Labs provides the following LPS products:  https://listlabs.com/product-information/lipopolysaccharides/

Plasmids and RNAi:

… Depletion of Rae1 was performed using a double-stranded siRNA oligonucleotide with the sequence 5-CUCAGCAGUAACCAAGCGAUACAGA-3 (Rae1 siRNA; Stealth RNAi; Invitrogen). Cells were incubated with pertussis toxin (181214A1; List Biological Laboratories, Inc.) at 400 ng/ml for 4 h before analysis.

• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)

In vitro antibody activity and aggregation assays:

… To monitor ligand-binding activity, an indirect PTx ELISA was employed as reported previously^18,37. High-binding ELISA plates (Costar) were coated with pertussis toxin (PTx, List Biological Laboratories) at 0.75 µg/ml in PBS and incubated at 4°C overnight. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Demyelination model:

We created relapsing-remitting EAE (R-EAE) from C57Bl/6 mice (Charles-River). Each mouse was immunized subcutaneously (into the flanks) with 100µg of MOG35-55 (MEVGWYRSPFSRVVHLYRNGK), 400 µg of Mycobacterium tuberculosis (strain H37Ra; Difco) in complete Freunds adjuvant (CFA, Difco). One hundred nanogram of Bordetella pertussis toxin (List Biological Laboratories via Cedarlane) was given intravenously at the time of immunization and 2 days later. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Induction of EAE:

… The A_2AR KO mice were in congenic C57BL/6 background after backcrossing for more than 12 generations (Yu et al. 2005). … To induce EAE, 10 to 12weekold female mice were injected subcutaneously with 200g MOG₃₅₅₅ peptide (AC Scientific, China) emulsified in complete Freunds adjuvant (CFA, containing 8mg/ml Mycobacterium tuberculosis H37Ra). Reconstituted lyophilized pertussis toxin (200ng List Biological Laboratories, Campbell, CA, USA) was injected intraperitoneally immediately after the MOG injection and again 48h later. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Assessment of the in vivo stability and regulatory activity of P4induced FoxP3⁺ T cells:

For the MOG peptideinduced EAE model, 5 10⁶ P4induced or control iTreg cells were injected i.v. into CD45.1⁺ congenic C57BL/6 mice and the mice were immunized with MOG₃₅₅₅ peptide (100 µg/mouse) in complete Freund’s adjuvant. On the same day, pertussis toxin (100 ng/mouse, PTX from List Biological Laboratories (Campbell, CA)) was injected i.p. The immunization was repeated 7 days later. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

For active EAE induction, mice were immunized with 100µg MOG3555 peptide (MEVGWYRSPFSRVVHLYRNGK) in CFA injected subcutaneously, mice received pertussis toxin (List Biological Laboratory, CA) via intraperitoneally (i.p.). …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

In vivo cell depletion:

Treg cells were depleted by administration of diphtheria toxin (DT) to Foxp3^DTR mice (List Biological Laboratories). Fifty micrograms per kilogram of DT was administered intraperitoneally (i.p.) in a 200 L volume every other day for 12 days unless otherwise noted. Control animals received 200 L sterile PBS ip. DT administration results in greater than 95% reduction specifically of Treg cells in the spleen, LN’s, peripheral blood, and bone marrow (data not shown). DT treatment of control (C57BL/6) mice failed to induce any signs of B-cell activation, terminal differentiation, T_FH development, or autoantibody production (Supporting Information Fig. 1). CD4 depletion was achieved by administration of 250 g GK1.5 ip every 3rd day (Bio X Cell).

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae