Bacterial Toxin Research Citations

Cell culture, transfection, and intoxication:

Bacterial toxins were loaded into cells by endocytic uptake…The culture medium was changed to a depolarizing solution of HEPES buffered salt solution containing 55 mm KCl with NaCl reduced to 37 mm, plus either or both of tetanus toxin (10 nm; List Biological Laboratories)…

• Product #190 – Tetanus Toxin from Clostridium tetani

Animals:

To compare vocalizations emitted by hearing and deaf male mice, we used a mouse line that expresses DTRs in hair cells to induce deafness. CBA/CaJ mice had the human dtr gene inserted into the pou4f3 gene…We gave 49 P2 mice a systemic injection of DT (4 ng/g i.m.; List Biological Laboratories)…

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

1,25(OH)₂D₃ Treatment and EAE Induction:

…To induce EAE, mice were immunized subcutaneously with 200 g MOG emulsified in complete Freunds adjuvant (Difco, Lawrence, KS) supplemented with 5 mg/ml Mycobacterium tuberculosis H37Ra (Difco, Lawrence, KS). The mice were injected twice i.p. with 200 ng of pertussis toxin (List Biological Laboratories, Campbell, CA) at the time of immunization and 48 hours later. The mice were then monitored daily for clinical symptoms of disease, …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Conditioning and dorsal column lesions:

…Another set of C57BL/6 129/SvJ (n = 5) and Slpi^/ (n = 7) mice received only dorsal column lesions. At 5 weeks after surgery, 2 µl of 1% cholera toxin B subunit (CTB; List Biological) was injected into the right sciatic nerve of each animal, and the animals were transcardially perfused with 4% paraformaldehyde (PFA) 3 d later. Spinal cord sections were immunostained using goat anti-CTB antibody (1:2000; List Biological),…

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt
• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

Retrograde Labeling of Proprioceptive Afferent Collateral Projections:

For CTB labeling, p1416 animals were anesthetized by Avertin (0.4 g/kg body weight, administered i.p.), and 0.5 µl of a 1% solution of CTB (List Biologicals) was injected in axial, intercostal, body wall, or hindlimb muscles. After 5 days, animals were processed for analysis.

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

… Lyophilized PTX (List Biological Laboratories Ltd, Campbell, CA) was purchased from Sapphire Biosciences, New South Wales, Australia. All chemicals used for experiments were of molecular biology grade and used as received unless otherwise specified. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Analysis of N-glycans attached to transgenic N. benthamiana-expressed CTB:

… After electrophoresis, proteins were transferred to a poly(vinylidene difluoride) membrane. Blots were probed with goat anti-CTB antiserum (List Biological Laboratories) …

• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

Animals and disease induction:

EAE was induced as previously described please (21) by injecting 0.2 ml of emulsion containing 125 g of MOG ₃₅₅₅ peptide… In addition, the mice received 300 ng of pertussis toxin (List Biological Industries, …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

CFA/I and LT Antigens:

The CFA/I fimbriae were isolated as previously described (Byrd et al., 2003) and the LT was obtained from List Biological Laboratories (Campbell, CA).

Author did not indicate which specific lethal factor was utilized.  List Labs provides Product #172 (Anthrax Lethal Factor (LF), Recombinant from B. anthracis) and Product #169 (Anthrax Lethal Factor (LF-A), Recombinant from B. anthracis Native Sequence).

• Product #171 – Anthrax Protective Antigen (PA), Recombinant from B. anthracis

Botulinum neurotoxin type A was purchased from List Biological Laboratories, Inc. and used after reconstitution in PBS.