Bacterial Toxin Research Citations

Tissue Processing, Analysis, and Immunohistochemistry

Stellate ganglia:

… Tissue sections were washed in 10 mM Tris, 0.9% NaCl, 0.05% thimerosal in 10 mM phosphate buffer (TPBS), pH 7.4, containing 0.3% Triton X-100 for 3 10 min then incubated in 10% heat-inactivated normal horse serum (NHS, Invitrogen) in TPBS-Triton for at least 1 h. The sections were then incubated in goat anti-CTB antiserum (1:25000, List Biologicals) in TPBS-Triton containing 10% NHS for 3 days at room temperature. …

• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

Materials:

… Nicked anthrax PA 63 from B. anthracis was obtained from List Biological Laboratories Inc., Campbell, CA. …

Binding Experiments:

The binding of the His-tagged proteins to the C2II-channel and the binding component PA₆₃ was investigated with titration experiments similar to those performed previously to study the binding of 4-aminoquinolones to the C2II- and PA₆₃-channels and EF and LF to the PA₆₃-channel in single- or multi-channel experiments [38][40]. The aqueous phase contained always 150 mM KCl, buffered with 10 mM MES-KOH to pH 5.5 to pH 6. The C2II- and PA₆₃-channels were reconstituted into lipid bilayers. About 60 minutes after the addition of either activated C2II or PA₆₃ to the cis-side of the membrane, the rate of channel insertion in the membranes was very small. Then concentrated solutions of His-tagged proteins were added to the cis-side of the membranes while stirring to allow equilibration.

• Product #174 – Anthrax Protective Antigen, Activated (PA 63) from B. anthracis

Intra-ocular injections:

The B subunit of the Cholera Toxin complex (CTB) has been shown to be a highly sensitive anterograde tracer for RGCs (Angelucci et al., 1996; Reiner et al., 1996; Matteau et al., 2003), and therefore the preferred tracer for this study. Rats were first anesthetized with 25% isoflurane mixed with oxygen at a flow rate of 1l/min., using an isoflurane vaporizer (Smiths Medical, Dublin, OH, USA) While maintained at the appropriate level of anesthesia, subjects were subcutaneously injected with buprenorphine (0.06mg/kg rat weight). Subjects then received, via syringe, 56 2l injections of either unconjugated CTB in one eye, or fluorophor-conjugated CTB in both eyes.

The monocular injections were administered into the vitreous chamber of the left eye only, and comprised a 1% CTB solution (List Biological Laboratories, Inc., Campbell, CA, USA) mixed with 2% dimethyl sulfoxide (DMSO) diluted in sterile water. …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

La CTb lyophilise (… List Biological Laboratories, CA) est reconstitue dans 100µL de tampon phosphate (PB) 0,1M pH 6 dans lequel la CTb est charge positivement, permettant ainsi son injection par iontophorse.

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

Animals and induction of experimental autoimmune encephalomyelitis:

Female SJL/J mice were purchased from Harlan Laboratories (Borchen, Germany) and C57BL/6 J mice were purchased from Janvier (Le Genest Saint Isle, France). For the induction of EAE, female mice at age eight weeks, received subcutaneous injections in both flanks of either 50 µg MOG_35-55 peptide or 100 µg PLP_139-151 peptide … Two injections of pertussis toxin (List Biological Laboratories Inc., Campbell, CA, USA; 200 ng per mouse intraperitoneal) were given on the day of immunization and 48 hours later. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Reagents and cell lines:

The following reagents were used: LPS from Escherichia coli O111:B4 (List Biological Laboratories), …

• Product #201 – LPS from Escherichia coli O111:B4

EAE Induction:

… Mice also received two i.p. injections with 200 ng of pertussis toxin (List Biologicals) at the time of immunization and 48 hours later. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Induction and Scoring of EAE:

EAE was actively induced by subcutaneous administration of 200 µL suspension at 2 sites over the hind flanks. The suspension consisted of 300 µg MOG₃₅₅₅ peptide (Sigma Aldrich, Germany) in 100 µL of PBS, emulsified with 100 µL complete Freund`s adjuvant (Sigma Aldrich, Germany) with 0.7 mg heat-inactivated Mycobacterium tuberculosis (strain H37 RA; Difco Laboratories, Detroit, MI). Each mouse was immediately thereafter, injected intraperitonealy and 48 hours later with 300 ng pertussis toxin (List Biological Laboratories, Campbell, USA) in 100 µL 0.9% NaCl. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Reagents:

… and Bordetella pertussis toxin (Ptx) was obtained from List Biological (Campbell, CA). …

Induction, Treatment and Scoring of EAE:

Six to eight week old female SJL/J mice were immunized s.c. with 200 µg (PLP)₁₃₉₁₅₁ peptide emulsified in CFA in the rear flank. The mice also received 325 ng of Ptx i.p. immediately after immunization and again on day 2. Immunized mice received vehicle or 150 nmol/mouse 1V136 s.c. daily from day 6 to day 18. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Induction of EAE:

Female wild-type (WT) C57BL/6 or pik3cg^/ were immunized subcutaneously in the hind flanks and at the scruff of the neck with a total of 25 µg Myelin Oligodendrocyte Glycoprotein (MOG) peptide-₃₅₅₅ … Two hours prior to immunization, and two days after, the mice were intravenously injected with 300 ng of pertussis toxin (List Biological Laboratories Inc., California, USA) in endotoxin-free PBS. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)