Bacterial Toxin Research Citations

EAE induction:

EAE was induced in 6- to 8-week-old female mice by subcutaneous immunization with 100 g of MOG_35-55 peptide (MEVGWYRSPFSRVVHLYRNGK; NeoMPS, San Diego, CA) emulsified in CFA containing 200 g of heat-killed Mycobacterium tuberculosis H37RA (Difco, Detroit, MI) on day 0. Additionally, mice received 200 ng of pertussis toxin (List Biological Laboratories, Campbell, CA) i.p. in 0.5 ml of PBS on days 0 and 2. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Surgery:

… Following optical imaging, the retrograde tracers Cholera toxin B (CTB, 10 g/l List Biological Laboratories) and CTB conjugated with gold (CTBG, 10 µg/µl List Biological Laboratories) were injected (Nanoliter 2000, World Precision Instruments) using a glass capillary (tip diameter 1012 m) into frequency-matched (NA1 = 8, BFA1 = 8.4 0.6 kHz, NcSRAF = 8, BFSRAF = 7.3 0.4 kHz, t(16) = 1.56, p = 0.14) contours in A1 and cSRAF. Injections targeted layer IV in both areas and had a cortical surface separation of 1.8 0.05 mm. …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

For Western blots, whole-cell lysates from 106 J774A.1 cells, separated by SDS/PAGE, were probed by anti-PA (List Biological Laboratories) and anti-actin antibodies (Santa Cruz Biotechnology) with chemiluminescence detection. …

Induction and evaluation of EAE:

Mice were immunized for the induction of EAE using a 2 or 1 immunization protocol … One week later, all mice received an identical injection of MOG₃₅₅₅-CFA. For the 1 immunization protocol, mice received a sonicated emulsion of 200 g MOG₃₅₅₅ and CFA containing 200 g Mycobacterium tuberculosis H37RA by subcutaneous injections, immediately thereafter, each animal received 200 ng PTX (List Biological Laboratories) by intravenous injection. Mice were scored daily for clinical quantitative trait variables beginning at day 5 after injection as follows: …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Experimental Protocol:

… After the baseline scans, five sheep received a continuous 10 ng/kg/min intravenous infusion of Escherichia coli endotoxin (lipopolysaccharide, LPS, O55:B5, List Biological Laboratories Inc, California) while six did not.

In this manner, four regional pulmonary pathophysiological conditions were studied:

1. healthy lung, i.e., no LPS and no lung lavage (LPS, Lav)
2. lung exposed to bronchoalveolar lavage but not to endotoxin (LPS, Lav+)
3. lung exposed to systemic endotoxin but not to lavage (LPS+, Lav)
4. lung exposed to systemic endotoxin and bronchoalveolar lavage (LPS+, Lav+)

NOTE:  At the time this paper was written, Product #203 (5mg – LPS from Escherichia coli O55:B5) was utilized.

Product #203 (5 mg – LPS from Escherichia coli O55:B5) is no longer sold.

Product #203A (2.5 mg – LPS from Escherichia coli O55:B5) is available for purchase.

• Product #203A – LPS from Escherichia coli O55:B5

In vivo LPS treatment in mice:

Experiments were performed according to protocols approved by the Chancellors Animal Research Committee of the Office for Protection of Research Subjects at the University of California, Los Angeles. LPS (Escherichia coli strain O55:B5, lot number 20316A1) was purchased from List Laboratories. We determined that the concentration of 10 mg/kg in this lot was adequate to induce the white matter injury in C57BL/6 mice as described in the results. LPS was diluted in sterile saline to a concentration of 0.5 mg/ml and injected subcutaneously between the shoulders. Please see supplementary materials for detailed information on injection.

NOTE:  At the time this paper was written, Product #203 (5mg – LPS from Escherichia coli O55:B5) was utilized.

Product #203 (5 mg – LPS from Escherichia coli O55:B5) is no longer sold.

Product #203A (2.5 mg – LPS from Escherichia coli O55:B5) is available for purchase.

• Product #203A – LPS from Escherichia coli O55:B5

Reagents, bacterial strains, and cells:

Ultrapurified LPS from Salmonella enterica subsp. enterica serovar Minnesota R595 (S. Minnesota) was from List Biological Laboratories, Inc. (Campbell, CA). …

Stimulation with TLR ligands and S. aureus:

The following general stimulation program was followed unless otherwise stated. RAW 264.7 cells (1 106) or BMDMs (see the supplemental material) were seeded in 12-well plates in 1 ml of complete RPMI medium. After 3 h, the cells were incubated with 1 g/ml SMAMP for 1 h and then stimulated with the following concentrations of TLR ligands: LTA (10 g/ml), LPS (100 ng/ml), poly(IC) (1.0 g/ml), …

• Product #434 – ULTRA PURE LPS from Salmonella minnesota R595 (Re)

Materials and Methods:

… LPS no. 225 Salmonella typhimurium was from List Biological Laboratories (Campbell, CA), …

Immunouorescence microscopy:

BMM on 12-mm circular coverslips were stimulated overnight with IFN-g or LPS, then prepared for immunouorescence microscopy. Cells were xed for 60 min at 37C (xative: 2% paraformaldehyde, 4.5% sucrose, 20 mM HEPES, pH 7.4, 70 mM NaCl, 10 mM KCl, 10 mM MgCl2, 2 mM EGTA, 70 mM lysine-HCl, 10 mM sodium periodate), rinsed …

• Product #225 – LPS from Salmonella typhimurium

Generation of EAE mice:

… EAE was induced as described previously (30). Briefly, SJL/J mice were injected subcutaneously in the abdominal flank on day 0 with 200 g of PLP 139151 and 400 g Mycobacteria tuberculosis H37RA (Difco, Detroit, MI) in 200 l of an emulsion of equal volumes of water and complete Freund’s adjuvant (CFA) (Difco). Age-matched control mice were injected with water and CFA only. On days 0, 3, and 7, each mouse was injected intraperitoneally with 0.2 g of purified Bordetella pertussis toxin (List Biological Laboratories, Campbell, CA). …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Antigen specific ELISA:

Antigen-specific IgG levels and total IgG were measured by ELISA as previously described[7, 8]. Antigens included … and pertussis toxin (PT, List Biological Laboratories, Campbell, CA). … Each ELISA plate included an internal standard/positive control sample for normalization between plates and between experiments performed on different days. Serum antibody titers of >120 ELISA units (EU) were considered seropositive.

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)