Citations

Bacterial Toxin Research Citations

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5030 citations found

Product: Anti-Cholera Toxin B Subunit (Goat)

  • … Frozen sections of the brain, the TG, and the eye muscles were processed for the immunocytochemical detection of the tracers cholera toxin subunit B and wheat germ agglutinin (goat anti-choleragenoid, 1:5000; List Biological Laboratories, Campbell, CA, USA; …

Selective corticospinal tract injury in the rat induces primary afferent fiber sprouting in the spinal cord and hyperreflexia

Tan, AM;Chakrabarty, S;Kimura, H;Martin, JH;

Product: Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

  • Muscle afferent tracing:

    Three days after PTx injury, animals were reanesthetized and muscle afferents of the deep radial nerve were labeled with 10 µl injections of a 1% solution of cholera toxin B (CTB) (List Biological Laboratories) into the extensor carpi radialis of both forelimbs. The solution was injected over 1 min, and the needle was withdrawn over an additional 1 min. The wound was closed by suturing the overlying muscle and skin. Preliminary data demonstrated that CTB transganglionically labeled both primary sensory afferents and retrogradely labeled motoneuron pools. Anterograde labeling is punctate. Whereas at high magnification we could see punctate ventral horn labeling in proximity to labeled motoneurons, we were not able to distinguish this labeling from other ventral horn label, including portions of motoneuron dendrites and labeled recurrent motoneuron axon collaterals. For this reason, we limited our quantitative CTB analysis to the dorsal horn and intermediate zone. To assay putative 1A boutons on motoneuron cell bodies and proximal dendrites, we examined VgluT1 presynaptic labeling. …

    Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

Dietary resveratrol prevents the development of food allergy in mice

Okada, Y;Oh-oka, K;Nakamura, Y;Ishimaru, K;Matsuoka, S;Okumura, K;Ogawa, H;Hisamoto, M;Okuda, T;Nakao, A;

Product: Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

Product: Anti-Cholera Toxin B Subunit (Goat)

  • Injection of tracers:

    For the immunohistochemistry experiments requiring retrograde tracing, six animals were anesthetized using 5% isoflurane with oxygen and placed in a stereotaxic apparatus (David Kopf Instruments, Tujunga, CA) and stabilized with non-perforating ear bars. … Two l of 1.0% solution of CTb (List, Campbell, CA) was slowly injected into the parabrachial nucleus over a period of 20 minutes, followed by a waiting period of 10 minute before the micropipette was retracted from its position to minimize leakage of the tracer. CTb was injected seven days prior to sacrificing the animals.

    Confocal microscopy:

    … The sections were incubated in 3 different cocktails: #1) rabbit anti-CGRP at a 1:200 dilution (Sigma, St Louis, MO) and lectin IB4 conjugated to AlexaFluor 568 at a 1:200 dilution (Molecular Probes); #2) rabbit anti-CGRP and guinea pig anti-P2X3 at a 1:25,000 dilution (Neuromics, Edina, MN); #3) goat anti-CTb at a 1:5000 dilution (List Biological), …

    Author did not specify which CTB was utilized.  List Labs provides Product #103B (Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae)  and Product #104 (Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt).

In situ differentiation of CD8 cells from CD4 T cells in peripheral lymphoid tissues

Nambu, Y;Hayashi, T;Jang, KJ;Aoki, K;Mano, H;Nakano, K;Osato, M;Takahashi, K;Itoh, K;Teramukai, S;Komori, T;Fujita, J;Ito, Y;Shimizu, A;Sugai, M;

Product: Pertussis Toxin from B. pertussis, Lyophilized in Buffer

Product: Pertussis Toxin from B. pertussis, Lyophilized in Buffer

Product: Anti-Cholera Toxin B Subunit (Goat)

  •  Table 1. List of immunological reagents, source and concentration

    Cholera toxin B subunit

    List Biologicals Campbell, California, USA 103B 5% 0.1 M PBS
    Guinea pig anti GABA ABcam Cambridge, UK ab17413 1:700 1% NGS0.3% Triton X-100-TBS
    Guinea pig anti vGLUT2 Millipore Oxford, UK MAB5504 1:12,000 1% NGS0.3% Triton X-100-TBS
    Rabbit anti nNOS Santa Cruz Santa Cruz, California, USA SC-648 1:400 1% NGS0.3% Triton X-100-TBS
    Goat anti CTB List Biologicals Campbell, California, USA 703 1:200,000 1% NHS0.3% Triton X-100-TBS

    Injection of retrograde tracers:

    Following recovery (710 days) from the NTS injection, animals were re-anaesthetised (as previously described) and either 1 l of 2% fluorogold (FG) or 2 l of 0.5% cholera toxin B subunit (CTB, Table 1) was pressure injected into the left side spinal cord intermediolateralis …

    Biotin dextran aminecholera toxin B subunit for epifluorescence:

    BDA sections were incubated in 10% NHS0.3% Triton-X-100 for 45 min, rinsed in Tris-buffered saline (TBS, 1  10 min) and then incubated in an appropriate streptavidin Alexa Fluor 594 or 488 for 2 h. The sections then had a further rinse in TBS followed by avidinbiotin blocking and incubation in goat anti CTB with streptavidin Alexa Fluor 594 or 488 … 

Reinduced Wnt signaling limits regenerative potential of sensory axons in the spinal cord following conditioning lesion

Hollis ER, Zou Y.

Product: Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

Product: Pertussis Toxin from B. pertussis, Lyophilized in Buffer

Sensitive and Specific Bifunctional Assay for Botulinum Neurotoxin Type A

Suryadi, K.; Christian, T.; Shine, N.

Product: Receptor for Clostridium botulinum Type A Neurotoxin, GST-SV2c luminal domain