Bacterial Toxin Research Citations

… Cholera toxin (CT) was purchased from List Biological Laboratories, Inc. (Cambell, CA). …

Experimental design.

According to our standard PE sensitization protocol, mice were sensitized to PE by intragastric exposure to PE (6 mg PE, 200 µl/mouse) with CT (15 µg/mouse) on three consecutive days (day 0, 1, and 2) followed by weekly dosing (days 7, 14, 21, and 28). …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

Mucosal sensitization was achieved via intragastric gavage of 25 µg of cholera toxin (List Biological Laboratories, CA, USA) and 500 µg of peptic-tryptic-digest of gliadin (PT-gliadin) dissolved in 0.02 M acetic acid. …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

… TLR agonists included ultrapure LPS from Salmonella minnesota (TLR4; List Biological Laboratories, Campbell, Calif) and …

Measurement of pDC activation:

Human newborn cord and adult peripheral blood was stimulated with TLR agonists at 37C, with end-over-end rotation for 19 hours.

• Product #434 – ULTRA PURE LPS from Salmonella minnesota R595 (Re)

LPS treatment and organ harvest:

Endotoxemia was induced by a single intraperitoneal injection of highly purified lipopolysaccharide from Escherichia coli (UP-LPS, 5 mg/kg i.p., strain O111:B4, endotoxin activity level as assessed by chromogenic limulus amebocyte lysate (LAL) assay 2.575 10⁶ EU/mg and 6.75 10⁶ EU/mg, List Laboratories) under short isoflurane narcosis while sham treatment consisted of anesthesia and vehicle injection (0.9 % saline). Functional and molecular studies were conducted 24 hours after sham or UP-LPS injection unless otherwise stated.

• Product #421 – ULTRA PURE LPS from Escherichia coli O111:B4

RelA Translocation Assay OT-II CD90:

1+ CD4+ T cells were isolated from spleen and lymph nodes with a CD4+ T cell isolation kit (Myltenyi Biotec) and 0.5-1 x 106 cells were adoptively transferred into CD90.2+ wild-type recipients.  The next day, recipient mice were injected intravenously with 5 g of Escherichia coli LPS (List Biological Laboratories) diluted in PBS and then a day later, some mice were iinjected intravenously with 100 g of chicken ovalbumin
peptide 323-339 (OVAp) (Invitrogen) diluted in PBS. …

Author did not specify which List Labs LPS product was utilized in their research.  List Labs provides the following LPS products:  https://listlabs.com/product-information/lipopolysaccharides/

… For measurements of cytokine release in T84 cells, T84 cell monolayers were stimulated apically with serum-free medium containing LPS (10 g/ml; List Biological Laboratories, CA, USA), and basolaterally with TNF- …

Author did not specify which List Labs LPS product was utilized in their research.  List Labs provides the following LPS products:  https://listlabs.com/product-information/lipopolysaccharides/

LPS lung injury:

After induction of anesthesia, 100 l of a 1 mg/ml aqueous solution of LPS (List Biological Laboratories) was instilled via an intratracheal P10 catheter (Fisher). …

Author did not specify which List Labs LPS product was utilized in their research.  List Labs provides the following LPS products:  https://listlabs.com/product-information/lipopolysaccharides/

EAE:

… Mice also received a co-injection of 500 µL Bordetella pertussis toxin (List Biological Laboratories, Cedarlane, Hornby, Ontario) on day 0 and day 2 post-immunization. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… For the study of pertussis toxin (IAP) pretreatment, sub-confluent monolayers of NRK cells were incubated for 24 h in the presence of IAP (100 ng/ml) (List Biological Lab. Inc., Campbell, Ca) for in vivo ADP ribosylation of G subunits in cells [15]. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Experimental Autoimmune Encephalomyelitis:

Mice were immunized with MOG peptide 3555 (300 µg/mouse) and Mycobacterium tuberculosis (500 µg/mouse) emulsified in Complete Freunds Adjuvant subcutaneously, in a volume of 0.1 ml/mouse over two sites: the right draining inguinal and axillary lymph nodes. One week later a booster immunization was delivered subcutaneously, over the contralateral draining lymph nodes. Pertussis toxin (500 ng/mouse) (List Biological Laboratories, Inc., Campbell, CA) was injected intraperitoneally on days 0 and 2 …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)