Citations

Antibodies and reagents:

… LPS (List Biological Laboratories), … were used to stimulate BMDCs.

Author did not specify which List Labs LPS product was utilized in their research.  List Labs provides the following LPS products:  https://listlabs.com/product-information/lipopolysaccharides/

Reagents:

LPS were from Escherichia coli O111:B4 (List Biological Laboratories, Campbell, CA, USA), …

Results – MLK4 does not interact with Cdc42, TRAF2 or TRAF6:

… TRAF6 is an effector protein recruited to the TLR4 receptor complex after cells are stimulated with LPS. …

• Product #201 – LPS from Escherichia coli O111:B4

Antigens:

Two of the PT antigen … obtained from commercial sources, List Biological Laboratories Inc. (List) (Campbell, CA) …

PT was utilized in:  Coating optimization testing for PT antigens

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EAE:

… supplemented with 3 mg/ml M. tuberculosis H37Ra (BD Difco, Detroit, MI; 400 µg/mice). Mice were injected with 400 ng of pertussis toxin (List Biological Laboratories, Campbell, CA) via a tail vein at the same time of immunization and again 48 hrs later. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Spinal Application of Pertussis Toxin and Cholera Toxin:

Bacterial toxins, pertussis toxin (PTX) and/or cholera toxin (CTX) (List Biological Laboratories Inc., Campbell, CA, for both), were injected via the intrathecal cannula to block Gi/Go and Gs proteins, respectively. PTX (10 µg/5 µl) was injected every other day (days 1, 3, and 5) during the last 5 days before sacrifice. CTX (5 µg/5 µl) was injected once 24 h before sacrifice.

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Mouse models of demyelination:

Age-matched C57BL/6 mice were used in two distinct mouse models of demyelination. … Immediately following CFA injections, and 24 hours later, mice were injected intraperitoneally with 200 ng of inactivated pertussis toxin (List Biologicals, Campbell, CA). One week after induction of EAE mice received a booster of MOG peptide in Incomplete Freunds Adjuvant (IFA) and control mice were injected with IFA without the MOG peptide. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… Bordetella pertussis toxin (PTX) was from List Biological Laboratories (Campbell, CA, USA) …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Induction and Evaluation of EAE:

Mice were immunized s.c. in the flanks with 200 µg of myelin oligodendrocyte glycoprotein (MOG)_35-55 peptide (synthesized by the Tufts University Core Facility Laboratory) in a 200µL emulsion of complete freund’s adjuvant (CFA) containing 5 mg/mL heat-killed Mycobacterium tuberculosis H37Ra extract (Sigma-Aldrich, St. Louis, MO) on day 0 and i.p. injected with 200 ng per mouse of pertussis toxin (List Biological Laboratories, Campbell, CA) on days 0 and 2. The clinical symptoms were scored daily from day 0 to 30 after immunization, as follows: …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Experimental autoimmune encephalomyelitis disease models:

Mice were immunized with MOG3555 peptide emulsied in CFA (Sigma) containing 4 mg/ml Mycobacterium tuberculosis extract H37Ra (Difco) in 100 ml/mouse s.c. distributed between the two hind anks and above the sternum on day 0. On days 0 and 2, 100 mg pertussis toxin (List Biological Laboratories) was given i.v. Mice were monitored daily and clinical scores were given as follows: …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Regulatory T cell transfer and Foxp3+ T cell ablation:

Spleens and lymph nodes from either WT or Foxp3DTR B6 3 B6.g7 mice were harvested, and polyclonal CD4+ T cells were puried by negative selection using the CD4+ T cell isolation kit and an LS column (Miltenyi Biotec). To deplete Foxp3-expressing cells from reconstituting polyclonal Foxp3DTR CD4+ T cell populations, DT (List Biological, Campbell, CA) was injected i.p. at a concentration of 50 mg/kg body weight on days 8, 10, and 12 after CD4+ T cell reconstitution (19). …

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae