Bacterial Toxin Research Citations

… Immature DCs with or without nicotine (NiDCs and MoDCs, respectively) were cultured with 10 ng/ml lipopolysaccharide (LPS; Salmonella minnesota; List Biological Laboratories, INC, Campbell, CA) to induce cytokine and chemokine production. …

• Product #304 – LPS from Salmonella minnesota R595 (Re)

Chemical inhibition of RhoGTPases:

Difficile toxin B (TcdB, List Biologicals, Campbell, CA) was used to pharmacologically inhibit Rho, Rac and Cdc42 …

• Product #155 – Toxin B from Clostridium difficile

ELISA:

All ELISAs were read at 450 nm using a 1420 Victor3 (PerkinElmer) plate reader, unless otherwise indicated. Total Ig in cell culture supernatant was quantied by IgM, IgG, or IgA ELISA kits (Zeptometrix), used according to manufacturers instructions. To monitor Ag-specic Ig production, 96-well Maxisorp plates (Nunc) were coated with either heat-killed Opa2 gonococci (N302), keyhole limpet hemocyanin (KLH; Sigma-Aldrich), or tetanus toxoid (TT; List Biological Labs) resuspended in pH 7.4 PBS (Wisent). …

• Product #191 – Tetanus Toxoid from Clostridium tetani

Animal experimentation:

… For immunization, mice received 500 mg H. pylori SS1 whole-cell sonicate along with 10 mg cholera toxin (CT; List Biologicals, Campbell, CA) by oral gavage in a total volume of 200 ml three times at weekly intervals. …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

Mice and Treg depletion:

… To deplete Treg cells, DEREG mice were injected with DT (List Biological Laboratories). Mice were injected i.p. with 1 mg DT every other day over a period of 9 d starting with the rst injection 1 d prior MHVA59 infection. For adoptive transfer experiments, recipients were treated with DT on days 5, 7, and 9 postinfection (p.i.). Control mice (B6) received the same DT treatment as DEREG mice. 2D2 3 DEREG donor mice were injected with 1 mg DT 1 d prior to cell isolation.

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

… and the retrograde tracer CTb (low salt, 1% in distilled water; List Biological Laboratories, Campell …  #104, …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

… Immunization was performed by mixing MOG peptide in complete Freund adjuvant containing 5 mg/mL heat-killed H37Ra, strain of Mycobacterium tuberculosis (Difco Laboratories, Detroit, MI). Pertussis toxin (200 ng, List Biological Laboratories, Campbell, CA) in PBS was administered iv on the day of immunization and 48 h later. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Reversible inactivation of FLS2 cortex:

… Initially, the FLS1 cortex was micro-injected with 0.2 µl of the retrograde tracer cholera toxin subunit (1% CTB dissolved in 0.1 M PBS; List Biological Laboratories). …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

Cell culture:

Cells were cultured at 37C and in 5% CO₂ in a humidified incubator. Primary mouse BMDMs were prepared as previously described (Gilchrist et al., 2006). In brief, femoral BM cells were plated on nontissue culturetreated plastic in BMDM medium … On day 7, cells were stimulated as indicated in the Results section, before being used for microscopy, flow cytometry, RNA extraction, or lipid extraction. Stimuli that were introduced into BMDM medium (as indicated in the text) were as follows: LPS (from Salmonella minnesota; List Biological Laboratories), …

• Product #304 – LPS from Salmonella minnesota R595 (Re)

Boosting immunization of mice.

On day 117, we randomly assigned primed mice in one of several cohorts to receive a boosting vaccination (Table 1). Some cohorts received CHO-BSA (10 g saccharide/immunization/mouse) transcutaneously (TCI) with or without 25 g of immunoadjuvantative cholera toxin (CT; List Biological Laboratories, Campbell, CA) or E. coli heat-labile toxin (LT) (9). We immunized additional cohorts with CHO-BSA (10 g saccharide/immunization/mouse) subcutaneously (SCI) with or without 5 g of immunoadjuvantative CT. … Control mice received CT or LT boosting alone or no boosting immunizations following oral priming. Additional control cohorts received no priming oral immunization and received only CHO-BSA with CT or LT transcutaneously or subcutaneously. When booster doses were given, they were administered at 2-week intervals for a total of four immunizations. Transcutaneous immunizations were carried out as previously described (32).

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae