Citations

Bacterial Toxin Research Citations

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5030 citations found

Interleukin 23 Production by Intestinal CD103+ Dendritic Cells in Response to Bacterial Flagellin Enhances Mucosal Innate Immune Defense

Kinnebrew MA, Buffie CG, Diehl GE, Zenewicz LA, Leiner I, Hohl TM, Flavell RA, Littman DR, Pamer EG

Product: Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

  • Mice and Reagents:

    Wild-type C57BL/6 mice were purchased from Jackson Labs. … Control mice received PBS. In vitro stimulation of single-cell suspensions from the lamina propria was performed using a concentration of 200 ng/mL of flagellin. Diphtheria toxin (List Biological Laboratories) was administered every other day at a dose of 10 ng/g starting 6 days prior to injection of flagellin. Mice received a total of 3 doses.

Product: Anthrax Protective Antigen (PA), Recombinant from B. anthracis

Human genetic variation altering anthrax toxin sensitivity

Martchenko, M;Candille, SI;Tang, H;Cohen, SN;

Product: Anthrax Protective Antigen (PA), Recombinant from B. anthracis

  • Chemicals and Reagents:

    PA and LF were purchased from List Biological Laboratories.

    Toxin Treatment and Cell Viability Assays:

    Mouse macrophages were treated with toxin for 4 h and human B lymphocytes for 48 h. Determination of macrophage viability was done by MTT assay as described in refs. 31, 32, whereas lymphocyte viability was determined by Resazurin (Invitrogen) fluorescence, as described by the manufacturer. Each data point shown in Figs. 1 and and22 represents the average and SD of results from three wells. Cell viability is shown as the percentage of survivors obtained relative to treatment by PA alone (100%). LD50 was calculated for each cell line and normalized to account for variation in toxin potency from the two different lot numbers used, as determined in five and three independent LD50 measurements of a control cell line for the first and second lots, respectively.

    Immunofluorescence Microscopy:

    PA protein was labeled with Alexa Fluor 647 using the protein labeling kit (A20173; Molecular Probes). We determined by MTT assay that such labeling did not affect the ability of PA/LF to kill macrophages. Fresh serum-free IMDM media was added to cells that contained 1 g/mL PA/Alexa Fluor 647. Cells were incubated for 20 min either at 4 C for PA-binding analysis or at 37 C for determination of PA internalization.

     

    Biochemical Assay of PA Binding and Internalization:

    Three million macrophage cells were exposed to 1 g/mL of PA at 4 C for 1 h for binding assay or at 37 C for 20 min for internalization assay. Cells were then washed with PBS solution three times and lysed in RIPA buffer containing a protease inhibitor mixture (Roche). Western blot analysis was performed using mouse monoclonal anti-PA antibody PA (C3) sc-52129 (Santa Cruz Biotechnology), or mouse antiactin monoclonal antibody (Sigma-Aldrich). Chemiluminescence of bands and their relative intensities were revealed using a VersaDoc 1000 instrument (Bio-Rad).

    Author did not indicate which specific lethal factor was utilized.  List Labs provides Product #172 (Anthrax Lethal Factor (LF), Recombinant from B. anthracis) and Product #169 (Anthrax Lethal Factor (LF-A), Recombinant from B. anthracis Native Sequence).

Ablation of neurons expressing agouti-related protein, but not melanin concentrating hormone, in leptin-deficient mice restores metabolic functions and fertility

Wu, Q;Whiddon, BB;Palmiter, RD;

Product: Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

  • Animals and DT Treatment:

    …Before behavioral experiments, mice were maintained on standard laboratory chow (5053; Lab Diet) that was available ad libitum. When the Lepob/ob;AgrpDTR/+ and Lepob/ob;PmchDTR/+ mice were 6 wk old (weighing 2025 g), 8 wk old (weighing 3540 g), or 10 wk old (weighing 4045 g), they were individually housed and switched to another standard chow diet (D12450B; Research Diets) for 7 d before being injected with DT. To ablate AgRP or MCH neurons, DT was injected twice per mouse (i.m., 2 d apart; List Biologicals). Body weight and food intake were recorded daily for several weeks. The DT dose was determined based on body weight at the time of injection, with 50 g/kg for mice weighing <40 g and 40 g/kg for mice weighing >40 g. …

Product: Anti-Cholera Toxin B Subunit (Goat)

  • Materials and Methods:

    … CTB was visualized by sequential incubations in a primary goat anti-CTB antibody (List Laboratories; 1:30,000 in 0.3% Triton X-100 in PBS). This antibody was raised against purified choleragenoid and does not result in labeling following preabsorption of the antibody with excess concentration of choleragenoid (Stocker et al., 2006), and no labeling is seen in material in which a CTB injection has not been performed (Kubke et al., 2004). Incubation in the primary antibody was followed by …

Cortical and subcortical connections of V1 and V2 in early postnatal macaque monkeys

Baldwin, MK;Kaskan, PM;Zhang, B;Chino, YM;Kaas, JH;

Product: Anti-Cholera Toxin B Subunit (Goat)

  • Histology:

    … Every third section of cortex was processed for cytochrome oxidase (CO; Wong-Riley, 1979b), to reveal the V1V2 border and the CO-dense stripes of V2, and another series of every third section was processed for CTB by using an antibody and the histological procedures described in Bruce and Grovfova (1992). See Table 2 for the CTB antibody source. The CTB antibody failed to stain cells in tissue from animals without CTB injections. A third series was processed for myelin, or set aside. Every third section of the thalamus was processed for CO to help determine lateral geniculate nucleus (LGN) and pulvinar borders. A second series of every third sections was processed for CTB, and the third series was set aside.

    … Table 2. Antibody Used in This Study. Antigen, Immunogen, Manufacturer, Dilution. Cholera toxin subunit B (CTB), Purified CTB isolated from Vibrio cholerae, List Biological Laboratories (Campbell, CA), goat polyclonal #703, 1:5,000. …

Clinical and pathological effects of intrathecal injection of mesenchymal stem cell-derived neural progenitors in an experimental model of multiple sclerosis

Harris, VK;Yan, QJ;Vyshkina, T;Sahabi, S;Liu, X;Sadiq, SA;

Product: Pertussis Toxin from B. pertussis, Lyophilized in Buffer

Product: Toxin A from Clostridium difficile

  • Animal procedures:

    Adult BALB/c WT mice were purchased from Jackson Laboratory (Bar Harbor, ME). Ad5 vectors were injected intramuscularly (IM, into the tibialis anterior of the right hindlimb, total volume 25 l) into 8 weeks old male mice after performing proper anesthesia with isofluorane. A total of 1 1010 vp per mouse was administered IM. Number of animals used for each experiment is specified on corresponding figure legend. Toxin A challenge experiments were performed at 14 dpi as previously described [14] by injecting 300 ng of freshly reconstituted toxin A (List Biological Laboratories Inc., Campbell, CA …

Chondroitinase ABC promotes selective reactivation of somatosensory cortex in squirrel monkeys after a cervical dorsal column lesion

Bowes, C;Massey, JM;Burish, M;Cerkevich, CM;Kaas, JH;

Product: Anti-Cholera Toxin B Subunit (Goat)

  • Spinal cord and brainstem:

    … Alternating horizontal sections were reacted for cytochrome oxidase, enabling clear demarcation of the gray and white matter. The other sections were incubated with primary goat anti-CTB (1:4,000) (#703; List Biological Laboratories) diluted in Tris-buffered saline containing 0.25% (vol/vol) Triton X-100 (Sigma) and 2.5% (vol/vol) normal rabbit serum (Millipore) to visualize the forelimb primary afferents and terminal fields. The brainstem was separated, blocked, and left in …

Product: ULTRA PURE LPS from Escherichia coli O111:B4