Bacterial Toxin Research Citations

2.2. EAU induction

EAU induction followed previously established protocols [[27], [28]]. Each mouse received a subcutaneous injection of 300 μg of human interphotoreceptor retinoid-binding protein peptide (hIRBP651–670; sequence LAQGAYRTAVDLESLASQLT) on both flanks. The peptide, supplied by Shanghai Hanhong Chemical Co., Ltd.,was emulsified in Complete Freund’s Adjuvant (CFA) that included 3.5 mg/ml of heat-killed Mycobacterium tuberculosis H37RA. This procedure was performed on day 0. Additionally, each mouse received 200 ng of pertussis toxin from List Biological Laboratories (Campbell, CA) in 200 μl of PBS via tail vein injection on days 0 and 2.

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Immunocytochemistry

After post-fixation, brains were submerged in 10% sucrose PBS solution at 4°C until they sank. The brains were then embedded in 14% gelatin and 10% sucrose, after which they were fixated in 10% formalin and 30% sucrose overnight, and subsequently in 30% sucrose until they sank. The brains were then cut in 50 μm sections. For light-microscopic staining of the CTb, the sections were first incubated for 20 min in 3% H2O2 (in PBS) to remove endogenous peroxidase activity of blood. Next, the sections were blocked in 10% normal horse serum (NHS) in PBS with 0.5% Triton, after which they were incubated at room temperature with anti-CTb (goat, 1:15,000, List labs) primary antibody (in PBS with 2% NHS, 0.5% Triton) for 48–72 hr and next with biotinylated rabbit anti-goat (1:200, Vector) secondary antibody (also in PBS with 2% NHS and 0.5% Triton) for 1.5 hr. …

• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

2.2. EAE induction
EAE was induced in 8- to 10-week-old female C57BL/6J mice by injecting 250 μg of MOG35-55 peptide (Genemed Synthesis) emulsified in complete Freund’s adjuvant (CFA) (BD Difco) per mouse, injected subcutaneously into each flank, followed by intraperitoneal administration of 150 ng of pertussis toxin (List Biological Laboratories, Inc.) per mouse on day 0. A second dose of 300 ng pertussis toxin was administered on day 2. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

White blood cell profile detection

For inducible ablation of CD26+ cells at the injury sites, each mouse was locally injected with 50 ng diphtheria toxin ((List Labs) daily for 5 days. At day 3 after last time of DTx injection, peripheral blood from PBS and DTx group were collected. White blood cell profiles were detected with auto hematology analyzer (BC-5000 Vet) according to operation manual.

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

… Under isoflurane gas anesthesia (1%-5.0% isoflurane/1.5 liter per minute O 2 ), each animals received 80µl injections of CTb neuronal tracer (List Biological Laboratories) …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

ELISA

Tachyzoites were washed in PBS before use in infection experiments, in order to avoid transferring any IL-1β from the parasite cultures. Confluent HFFs in 24 well plates were then infected with 5 × 105 freshly lysed tachyzoites. THP-1 cells were plated in 24-well plates at 5 × 105 cells/well and infected at a MOI = 2 or stimulated at the same time with 100 ng/mL of E. coli LPS (List Biological Laboratories, Campbell, CA) and with 5 mM ATP (Sigma-Aldrich, St. Louis, MO) added in the 30 min prior to harvest. …

Author did not specify which List Labs LPS product was utilized in their research.
List Labs provides the following LPS products: https://www.listlabs.com/product-information/lipopolysaccharides/

4.2 Intragastric Model of Sensitization

Mice were sensitized with either 3.75 mg All-Natural Smooth Peanut Butter (Kraft Heinz, Northfield, USA), 1 mg ovalbumin (OVA) (MilliporeSigma, A5378), or 1 mg 4-hydroxy-3-nitrophenyl (NP)-OVA (LGC Biosearch Technologies, N-5051-100) by oral gavage with 5 μg cholera toxin (List Biological Labs, 100B) in 0.5 mL PBS. …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

2.2 EAE induction

EAE was induced in the EAE and EAE-ACE groups through active immunization at upper/lower back (100 μL emulsion/site). The mice were subcutaneously injected with 200 μg MOG35–55 emulsified in 200 μL of complete Freund’s adjuvant (CFA, Sigma F5881, United States), containing 5 mg/mL of the Mycobacterium tuberculosis strain H37Ra (BD 231141, United States). The emulsion was prepared using phosphate-buffered saline (PBS) under sterile conditions. Additionally, 250 ng pertussis toxin (PTX181, List Biological Laboratories, United States) was administered intraperitoneally on the day of immunization and 2 days later. …

• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)

Neuronal Ablation

Mice carrying the En1Cre;hCdx2::FlpO, Chx10Cre;hCdx2::FlpO, Hes2iCre;hCdx2::FlpO, En1Cre;Chx10Cre;hCdx2::FlpO alleles in addition to the effector allele Tauds-DTR received i.p. injections of diphtheria toxin (DTx, 50 ng/gram of weight; List Biological Laboratories) at P28 and P31. Hes2iCre;VGATFlpO;Tauds-DTR mice and littermate controls Hes2iCre;Tauds-DTR mice received intrathecal injections of diphtheria toxin (DTx, 10 ng; List Biological Laboratories) at P28, P30 and P32. FlpO negative mice injected with DT were used as controls.

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

… The retrogradely spreading cholera toxin B subunit (CTB; List Biological Laboratories, Campbell, CA) was used to reveal the afferent neuronal connections of the LSv and …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt