Bacterial Toxin Research Citations

Pertussis toxin List Biological Laboratories Cat# 181

Experimental autoimmune encephalomyelitis (EAE)

For active EAE induction, Jak1ΔDC and Jak1WT littermate controls were immunized by s.c. injection of 75 μg myelin oligodendrocyte glycoprotein (MOG)35–55 peptide (Charitè Berlin, Germany) emulsified in 75 μg CFA (IFA (#F5506, Sigma) enriched with 10 mg/ml Mycobacterium tuberculosis, (H37Ra, #231141, BD). Pertussis toxin (200 ng, #181, List Biological Laboratories) was administered i.p. on day 0 and day 2 post immunization.

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… The BLG-sensitized group was gavaged with 200 µL sodium carbonate/bicarbonate buffer (pH 9.0) containing 10 µg cholera toxin (CT; List Biologicals, Campbell, CA, USA) as an adjuvant and 1 mg of purified BLG (Millipore Sigma, Burlington, MA, USA). The sham mice received only the vehicle (10 µg CT in 200 µL sodium carbonate/bica …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

… 2.10. Antibody Assay IgG and IgA antibody responses to the three bacterial substances, ultrapure E.coli-LPS (O111:B4) (List Biological Laboratories, Campbell, CA), ultrapure Pg-LPS (InvivoGen, San Diego, CA), and PG-PS (Lee Laboratories, Grayson, GA), …

• Product #421 – ULTRA PURE LPS from Escherichia coli O111:B4

… Active EAE was induced in 8 10-wk-old male and female mice by s.c. immunization with murine myelin oligodendrocyte glycoprotein (MOG)35-55 peptide (GenScript) and CFA, as previously described (36). Pertussis toxin (400 ng/mouse; List Biological Laboratories) was administered i.p. on days 0 and 2 following immunization. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Cell cultures

… 100 ng/ml cholera toxin (Cat#101B, List Biological Laboratories), …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

Materials

LPS from Salmonella typhimurium was purchased from List Biological Laboratories…

Cell culture and stimulation

… For LPS treatments, cells were pretreated with 100 ng/ml LPS for 30 min, followed by addition of FDx. For PMA treatments, cells were pretreated for 15 min with 100 nM PMA, followed by addition of FDx. For IL34 and CXCL12 treatments, cells were treated with FDx and 100 ng/ml IL34 or 50 nM CXCL12. …

• Product #225 – LPS from Salmonella typhimurium

Induction of EAE (active EAE): We used our standard protocol for the induction of EAE [35]. Briefly, six-to-eight-week old female mice were immunized with MOG35−55 in Complete Freund’s Adjuvant (CFA; Sigma-Aldrich) as previously described by us [35]. A total of 200 μg MOG35−55 peptide and 400 μg killed Mycobacterium tuberculosis (Difco Laboratories) was emulsified in CFA and injected subcutaneously (s.c.) into the flanks of the mice. Additionally, each mouse was injected with 200 ng pertussis toxin (PTX; List Biological Laboratories Inc., Campbell, CA, USA) intraperitoneally (i.p.) on day 0 and day 2 post-immunization. PTX is a critical component of the immunization procedure, and is widely used to induce EAE in C57BL/6 mice [35]. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Experimental Autoimmune Encephalomyelitis

EAE was performed as we have done previously35. Briefly, mice were injected s.c. with 100 ug MOG35-55 peptide (GenScript) emulsified in CFA. Mice were also injected i.p. with 300 ng pertussis toxin (List Biological Laboratories) on days 0 and 2. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… C. difficile toxin A (TcdA; List Biological Laboratories, Campbell, CA, USA) …

• Product #152C – Toxin A from Clostridium difficile

Drugs

Diphtheria toxin (DTX; List Biological Laboratories, Product 150) was dissolved in 93 0.9% saline to make 2 mg/ml stock solution and stored at -70° in aliquots. …

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae