Bacterial Toxin Research Citations

Differentiation and stimulation of THP-1 monocytes

… 1µg/ml protective antigen (PA; 90 min; List Biological Laboratories …

• Product #171E – Anthrax Protective Antigen (PA), Recombinant from B. anthracis

2.1. Mouse model and treatment

Vglut3DTR/+ mice were generated in our lab. We used the CRISPR/Cas9 approach to connect DTR to the C-terminus of Vglut3 via the P2A peptide. PCR and Southern blot methods were used to screen out the mice with the correct recombination. Lgr5CreERT2/+ (Stock #008875) and Rosa26-tdTomato (Stock #007914) mice were purchased from the Jackson Laboratory. The primers used for genotyping Lgr5creERT2/+, Rosa26-tdTomato and Vglut3DTR/+ mice are listed in Supplementary table 1. Tamoxifen (Sigma-Aldrich) was injected intraperitoneally at postnatal day (P)2 at 0.20 mg/g bodyweight. EdU (Click-iT EdU Imaging Kit; Invitrogen) was injected intraperitoneally from P3 to P5 once a day at a dose of 50 μg/g. DT (List Biological Laboratories) was injected intraperitoneally at 12.5 ng/g, 25 ng/g, or 37.5 ng/g at P2. …

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

Immunization of the replication cohorts followed the protocol of Wagnon et al. [17].

Male C57B/6 J mice were immunized at 8–9 weeks of age with either GluN1168-187 (control peptide), GluN1359-378 (active peptide) or for our additional comparison with ovalbumin, each emulsified in an equal volume of CFA (as described above). Antigens (200 µg) were equally distributed over 4 subcutaneous injections, 2 at shoulders and 2 at hind limbs. In addition, mice received 2 intraperitoneal injections of 200 ng of Bordetella pertussis toxin (#180, List Biological Laboratories) in PBS, immediately after and 48 h after immunization.

• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer

Animals and regents

Four- to five-week-old female C57BL/6 mice were obtained from the Experimental Animal Center of Guangdong (Guangzhou, China). … Pertussis toxin (PTX) was purchased from List Biological Lab (Epsom, England). …

EAE induction and assessment

EAE was induced as previously described immediately after the fecal collection [27]. Briefly, mice immunized subcutaneously in the flanks of 200 μg MOG35–55 peptide emulsified in CFA including 500 μg mycobacterium tuberculosis H37RA on day 0 and day 7. Immediately thereafter and on day 2, the mice received an intraperitoneal injection of 300 ng PTX in 100 μl PBS. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Diphtheria toxin (DT) administration.

Experimental mice (DTR mice, 6-9 weeks old) received 2 123 intramuscular injections of DT from Sigma-Aldrich (St. Louis, MO) or List Biological Laboratories, Inc. (Campbell, CA) at 25 ng/g, spaced 2d apart …

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

Study Design

This single-center, randomized, double-blind, placebo-controlled, full-factorial single-dose study was conducted between December 2016 and December 2017 in subjects in whom pyrexia was induced by intravenous (IV) administration of a reference standard endotoxin (RSE; ClinicalTrials.gov identifier: NCT02761980). In this standardized model, fever was induced by endotoxin (gram-negative bacterial lipopolysaccharide) derived from Escherichia coli O113, which was developed as a national biological reference standard in 1976 by the National Institute of Allergy and Infectious Diseases and the US Food and Drug Administration.17 The RSE used in this study was a new lot supplied by List Biological Laboratories, Inc. (Campbell, California). …

• Product #433 – LIST™ HPT™ from Escherichia coli O113

… The two tracers were fast blue (FB, Sigma-Aldrich, Gillingham, UK), and cholera-toxin b (CTB, List Biological Laboratories Inc, CA; 1% solution in 0.05M tris) …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

2.2. Experimental Design

… In the sensitization phase (day 0–28), mice in the PBS group were administered 10 µg of cholera toxin as an adjuvant (CT; List Biological Laboratories, Campbell, CA, USA) in 0.5 mL PBS via oral gavage. Mice in the other experimental groups were administered 20 mg whey together with 10 μg CT/0.5 mL PBS. …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

• Product #152C – Toxin A from Clostridium difficile
• Product #155 – Toxin B from Clostridium difficile

… Pertussis toxin (PTX, 200 ng, List Biological Laboratories) was injected intravenously immediately and 2 days after immunization. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)