SEB, superantigen of choice for positive control for cytokine detection
January 25, 2016
By: Grace Ayabe, Technical Support
Would you like a positive control for production of cytokines in human peripheral blood mononuclear cells (PBMC’s)? If your laboratory is testing for cytokine production, List Labs can provide you with SEB (Staphylococcus aureus, Enterotoxin Type B) to stimulate a positive response to assure that your cells are functional.
SEB is described as a ‘superantigen’ for its ability to bind to Major Histocompatibility Complex (MHC) class II molecules on antigen presenting cells and specific vβ regions of T-cell receptors stimulating a large population of T-cells; which in turn, produce an inappropriate flood of cytokines or a ‘cytokine storm’. Following SEB exposure, human peripheral blood cells release large quantities of IL-2, IL-4, IL-6, TNF-A and INF-c. SEB has also been found to bind to another regulator of the T-cell immune response, CD28. You can read more about T-cell proliferation with List Labs SEB here: https://www.listlabs.com/blog/staphylococcus-enterotoxin-b-stimulates-t-cell-proliferation/
If your research utilizes an Enzyme-linked immunospot (ELISPOT) or cytokine flow cytometry (CFC)/intracellular cytokine staining (ICS) assay(s) for cytokine detection, List Labs provides a premium quality SEB (Staphylococcus aureus, Enterotoxin Type B; Product #122) for your use as a positive control.
Each vial of List Labs SEB (Staphylococcus aureus, Enterotoxin Type B; Product #122) contains 0.5 mg of SEB in 0.07 M Sodium Phosphate; pH 6.8 (lyophilized). For more information, visit: https://www.listlabs.com/products/buy-enterotoxin-type-b-from-staphylococcus-aureus
SEB is a ‘Select Agent’. To order SEB from List Labs for the first time, please review the following link: https://www.listlabs.com/controlled-substances/