Bacterial Toxin Research Citations

… Immortalized human bronchial epithelial (HBE1) cells were maintained in DMEM/F12 media (Invitrogen, Carlsbad, CA) supplemented with 1 ng/ml cholera toxin (List Biological Laboratories, Campbell …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

Reagents

… Ultra-pure lipopolysaccharide (LPS) was purchased from List Biological Laboratories (Campbell, CA). …

Mouse cell culture

… To polarize BMDM, the culture media was first replaced with DMEM/F12, 10% heat-inactivated FBS, 100 U/ml penicillin G sodium, 100 μg/ml streptomycin sulfate, and 10 mM HEPES, and cells were incubated for 7 to 24 h. 100 U/ml IFNγ (Shenandoah Biotechnology Inc., Warwick, PA) and 100 ng/ml ultra- pure lipopolysaccharide (LPS) were added to polarize toward an M1 (inflammatory or classically activated) phenotype, and 10 ng/ml IL-4 (Shenendoah Biotechnology Inc.) was added to polarize toward an M2 (anti-inflammatory) phenotype. The cells were incubated overnight. In some experiments, polarization was confirmed by western blot for iNOS (M1) or Arginase 1 (M2), data not shown.

Author did not specify which List Labs Ultra Pure LPS was utilized.
List Labs provides the following Ultra Pure LPS:

• Product #421 – ULTRA PURE LPS from Escherichia coli O111:B4
• Product #423 – ULTRA PURE LPS from Escherichia coli O55:B5
• Product #434 – ULTRA PURE LPS from Salmonella minnesota R595 (Re)

Vaccine Composition.

Vaccine antigens (1.25 µg of genetically detoxified pertussis toxin (gPT) (LIST Biologicals, #184), 1.25 µg of filamentous hemagglutinin (FHA) (Enzo Life Sciences), and 0.8 µg of pertactin (PRN) (LIST Biologicals, #187)) were diluted with endotoxin-free Dulbecco’s PBS (Millipore Sigma) to make the base vaccine (aP). The mock-vaccine (MV) for this study was the vaccine diluent, endotoxin-free Dulbecco’s PBS (Millipore Sigma). The endotoxin content of the antigens was measured by the Limulus amebocyte test (PierceTM LAL …

• Product #184 – Pertussis Toxin Mutant
• Product #187 – Pertactin from B. pertussis (69 kDa Protein)

… We injected CTb (List Biological Laboratories) into the DLS. We injected 80 nl of 1% CTb unilaterally into the DLS over 4 min with the needle left in place for an additional 2 min (Li et al., 2018a) …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

Induction, treatment, and clinical evaluation of EAE

… Mice were anaesthetized with isourane and 120 μl of emulsion was injected subcutaneously on each flank (200 μl total/mouse) on day 0. In addition, 500 μl of pertussis toxin (1μg.ml -1; List Biological Laboratories, Campbell, CA) was injected into the tail vein on days 0 and 2. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… lipopolysaccharide (LPS) from Salmonella Minnesota R595 (Re) was obtained from List Biological Laboratories (Campbell …

• Product #304 – LPS from Salmonella minnesota R595 (Re)

Animals and deafening protocol

… Diptheria toxin (DT; List Biologicals Lab. Inc. cat. #150) was dissolved in saline, aliquoted and stored at − 20 °C until use. Repeat freeze–thaw cycles were avoided. A single dose of 20 ng/g DT was administered intra-muscularly to adult mice. Mice receiving adenovirus (Ad) inoculation surgery, were given DT immediately after the surgery. High-calorie diet gel (DietGel 76A, ClearH20) and an extra bottle of water were provided for the 2 following weeks. Body weight and general health metrics were monitored every day for a week after DT administration.

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

… For Treg depletion, diphtheria toxin (DT; List Biologicals, Campbell, CA) was administered intraperitoneally 2 days and 1 day before intratracheal instillation of bacteria and then on days 1, 3 after inoculation. …

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

Reagent:

Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae List Biological Laboratories Cat# 150

Tumor Associated Macrophage Co-Injection Experiments

Foxp3DTR-GFP donor mice were injected as mentioned above with either PyMT or EO771 cells, and their tumors were allowed to grow until approximately 90mm3. Half of the donors received two doses of 50 μg/kg of diphtheria toxin (DT) in two consecutive days, and primary tumors from both groups were harvested four days after initial DT treatment.

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

Experimental Autoimmune encephalomyelitis

… Pertussis toxin (120 ng) (List Biological Laboratories) diluted in PBS was administered i.v. on days 0 and 2 post-immunization. The clinical severity of EAE was scored by an observer who was unaware of mouse genotypes, using a grading scale of 0–5 as previously described.

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)