Bacterial Toxin Research Citations

… 1A) Principle for isolating CT-binding bacteria. CT (List Biological Laboratories, USA) previously dialyzed against PBS was directly linked to Dynabeads M-270 Amine (Life technologies, UK) according to manufacturer’s instructions …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

Ca2+ measurement

… To assess the role of G proteins in PAR2-mediated Ca2+ release, cells were pretreated either with PTX (100 ng/mL, 18 h; List Biological Laboratories) or YM-254890 (1 µM, 30 min; Wako Pure Chemical Industries) before agonist stimulation.

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Histology and microscopy

For vermal Purkinje cell retrograde tracing, goat anti-cholera toxin B subunit primary antibody (1:15000, List labs, 703) and biotinylated horse anti-goat secondary antibody (1:2000, Vector, BA-9500) were used. …

• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

… anthrax lethal factor (List Biological Labs #LL-169B) and protective antigen for anthrax lethal factor (LF) …

• Product #169L – Anthrax Lethal Factor (LF-A), Recombinant from B. anthracis Native Sequence
• Product #171E – Anthrax Protective Antigen (PA), Recombinant from B. anthracis

… Briefly, the mice were immunized sc with 200 µg MOG 35-55 peptide emulsified in CFA containing 5 mg/ml heat-killed Mycobacterium tuberculosis (Difco Laboratories) and received ip injection with 200 ng pertussis toxin (List Biological Laboratories, Inc., Campbell …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Animal Models

… For myeloid-depletion experiments, 250 ng DT (List Biological Labs, Campbell, CA) was injected intraperitoneally every other day for 4 days after DSS initiation, …

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

2.3. Drug Administration

Diphtheria toxin (List Biological Laboratories, Campbell, CA, USA), with a half-life of ~18 h, was dissolved in 0.9% saline and administered intramuscularly [13]. …

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

STAR★Methods
Key Resources Table

Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae List Biological Laboratories Cat# 150

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

STAR★Methods
Key Resources Table

Pertussis toxin List Biological Laboratories Inc. 180

Experimental autoimmune encephalomyelitis (EAE)

Rag1−/− recipients of 2D2 T cells were injected intraperitoneally with 200 ng per mouse of Pertussis toxin (List Biological Laboratories Inc.) reconstituted in PBS on days 1 and 3 after T cell transfer. Clinical disease score of EAE was graded on a scale of 0-4: 0 – no clinical signs; 1 – flaccid tail; 2 – hind limb weakness; abnormal gait; 3 – complete hind limb paralysis; 4 – complete hind limb paralysis and forelimb weakness or paralysis. Mice were scored daily in a blinded fashion.

• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer

A separate group of rats underwent stereotaxic surgery to receive unilateral injections of the retrograde tracer cholera toxin B (CTB, 0.2% dissolved in 0.9% NaCl, List Biological Laboratories) in RMTg …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt