Bacterial Toxin Research Citations

2.2. Reagents

… Ultrapure Lipopolysaccharide (LPS) was purified from Salmonella minnesota R595 (List Biological …

• Product #434 – ULTRA PURE LPS from Salmonella minnesota R595 (Re)

… A total of 50 µl emulsion was deposited on either side of the tail base. Pertussis toxin (300 ng per 200 µl; 180, List Biological Laboratories) was intraperitoneally injected on days 0 and 2 after MOG immunization …

• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer

2.5. Multiplex immunoassay

IgG concentrations specific to vaccine antigens PT, PRN, FHA, TT and DT were measured using an in-house multiplex bead-based immunoassay (MIA). IgG specific to FIM 2/3, which is not included in Boostrix, was also measured as a proxy for natural infection. PT, PRN and FHA were kindly provided by GlaxoSmithKline (Belgium). TT was purchased from Sigma-Aldrich. DT and FIM 2/3 were sourced from List Biological Laboratories (USA). A standard curve was generated using a 10-step 3-fold serial dilution of an in-house reference serum. Blanks and two quality control (QC) samples were included on every plate to calculate % critical variance across all assays, which fell between 5.1 and 14.6%. Assay specificity was determined using inhibition and interference assays. No cross reactivity was detected (Supplementary Figs. 1 and 2). …

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae
• Product #186 – Fimbriae 2/3 from B. pertussis

Antigens and biotinylation

… pertussix toxin (List Biological Laboratories), …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

STAR★methods
KEY RESOURCES TABLE
REAGENT OR RESOURCE Source Identifier

… Ultra-pure LPS List Biological Laboratories Cat# 225 …

ELISA
Mouse peritoneal macrophages were primed with ultra-pure LPS (3 ng/ml; List Biological Lab) for 3 hr and were then stimulated with crystals for 3 hr at 37°C. IL-1β in cell culture supernatants (sup.) was measured by ELISA (R&D Systems).

• Product #225 – LPS from Salmonella typhimurium

Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae List Biological Laboratories, INC. …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

… Pertussis Toxin List Biological Technologies Cat# 180 …

• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer

4-hydroxytamoxifen, diphtheria toxin, and tamoxifen treatment

… Diphtheria toxin (DT, List Biological Laboratories, 150) was dissolved in PBS. 1µg (first dose) or 613 250 ng (subsequent doses) was injected intraperitoneally for each mouse. …

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

… Pertussis toxin (salt free; 250 ng; List Biological Laboratories, Inc.) was injected intraperitoneally on day 0 and day 2 post-immunization. …

• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)

FACS analysis

Differentiated hiPSCs were analyzed by FACS as described previously (Hayashi et al., 2017, 2018). Cells were then dissociated using StemPro™ Accutase™ Cell Dissociation Reagent (Thermo Fisher Scientific) and resuspended in cold KCM medium (DMEM without glutamine and Nutrient Mixture F-12 Ham [3:1; Thermo Fisher Scientific] supplemented with 5% FBS [Japan Bio Serum, Hiroshima, Japan], 0.4 μg/mL hydrocortisone succinate [Wako], 2 nM 3,3′,5-triiodo-l-thyronine sodium salt [MP Biomedicals, Santa Ana, CA, USA], 1 nM cholera toxin [List Biological Laboratory, Campbell, CA, USA], 2.25 μg/mL bovine transferrin HOLO form [Thermo Fisher Scientific], 2 mM l-glutamine, 0.5% insulin transferrin selenium solution [Thermo Fisher Scientific], and 1% penicillin-streptomycin solution). …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae