Bacterial Toxin Research Citations

… To label abdominal premotor neurons in the caudal ventral respiratory group (cVRG), 6 rats were microinjected with the retrograde tracer cholera toxin subunit B (CTB, List Biologicals …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

… Test sera were duplicated and diluted two-fold starting with a 1:50 PA 500 ng/mL in lethal factor (LF) 400 ng/mL (List Biological Laboratories Inc., USA). …

Author did not specify which Lethal Factor (LF) was utilized; List Labs provides the following LF:

• Prodcut #172 – Anthrax Lethal Factor (LF), Recombinant from B. anthracis
• Product #169L – Anthrax Lethal Factor (LF-A), Recombinant from B. anthracis Native Sequence

Induction of EAE and Clinical Assessment of Animals

Active EAE was induced according to a standard protocol [35]. Mice were immunized on day 0 by subcutaneous injection on both sides of the tail base with 100 μL of an emulsion composed of either PLP139-151 or MOG35-55 peptide in an equal volume of Freund’s adjuvant, supplemented with 4 mg/mL killed M. tuberculosis H37Ra (Difco Laboratories, Detroit, MI). The initial dose of antigen was 100 μg of PLP peptide for inducing relapsing EAE in SJL/J mice, or 200 μg of MOG peptide per GFAP-luc/B6(Cg)-Tyrc-2J/J mouse for induction of acute monophasic EAE. GFAP-luc/SJL/J mice received a second PLP antigen immunization on day 7. Immediately following these immunizations, all mice received intravenous (IV) injections of 200 ng Bordetella pertussis toxin diluted in 200-μL phosphate buffered saline (PBS) (List Biological Laboratories, Campbell, CA). The GFAP-luc/B6(Cg)-Tyrc-2J/J mice received an additional IV injection of 200 ng Bordetella pertussis toxin on postinoculation day 2. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… according to the manufacturer’s instructions with 300 nM recombinant light chain from BoNT/C (#625A, List Biological Laboratories). After electroporation, the keratinocytes were washed and then seeded with sensory neurons …

• Product #625A – Botulinum Neurotoxin Type C Light Chain, Recombinant

… The assay mixtures were allowed to incubate at r.t. for 30 min, before 25 μL of SNAPtide substrate flP6 (List Labs) dissolved in assay buffer was added to give a final substrate concentration of 4 μM, initiating the reaction …

• Product #523 – SNAPtide® Peptide Substrate flP6(DABCYL/5-IAF) for C. botulinum Type A Neurotoxin

… and active and inactive FlaTox (2 mg/ml) in combination with protective antigen (2 mg/ml) (List Biological Laboratories Inc., catalog no. 171E) for NLRC4 inflammasome activation …

• Product #171E – Anthrax Protective Antigen (PA), Recombinant from B. anthracis

Experimental autoimmune encephalomyelitis

Female C57BL/6J mice (8 weeks old) were purchased from the Jackson Laboratory. EAE was induced and scored as previously described46. Briefly, female C57BL/6 mice were immunized subcutaneously with 50 μg of myelin oligodendrocyte glycoprotein peptide 35–55 (MOG35–55) (CSBIO #CS0681) emulsified at a 1:1 ratio in Complete Freud’s Adjuvant (Sigma Aldrich #F5881). On days 0 and 2, 250 ng of pertussis toxin (List Biologicals #180) was administered intraperitoneally. …

• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer

Methods

B. pertussis proteins

… Endogenous B. pertussis adenylate cyclase antigen and filamentous hemagglutinin were purchased from List Biological Laboratories (Campbell, CA).

B. pertussis strains

… 165 from List Biological Laboratories; …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

• Product #188L – Adenylate Cyclase Toxin, Recombinant from B. pertussis

… For active induction of EAE, … pertussis toxin (List Biological Laboratories) intraperitoneally on days 0 and 2 …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… Also, 300 ng of pertussis toxin (… List Biological Lab, USA) was intraperitoneally injected on day 0 and 48 h later [5]. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)