Bacterial Toxin Research Citations

… The medulla and cervical spinal cord were sectioned at 40 μm. Every second section from the hypoglossal region of the medulla and from C3-C5 within the cervical spinal cord was stained with an anti-cholera toxin antibody (List-Biologicals, 703) …

• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

… Two intraperitoneal injections of 400 ng pertussis toxin (List Biological Laboratories, Denver, CO, USA) were given 0 and 48 hours later …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… Mice were immunized on days 0, 7, and 14 by intraperitoneal injection of 100 µl of injectable grade PBS containing: PBS only (control groups), 20 µg LLO T in the presence or in the absence of 1 µg cholera toxin (List Biological Laboratories, Inc, Campbell, CA), …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

… Induction of EAM was performed as described previously [1, 19]. Briefly, the hind soles of rats were injected with 200 μl of human cardiac myosin (2 mg/ml) mixed with an equal volume of complete Freund’s adjuvant supplemented with 5 mg/ml Mycobacterium tuberculosis H37RA (Difco, Detroit, MI, USA). Experimental animals were intraperitoneally administered with 500 ng of pertussis toxin (List Biological Laboratories, Inc., Campbell, CA, USA) on the day of immunization day 0 and 2 days post-immunization (D2PI). …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EAE Induction

EAE induction was based on a published protocol [24]. Murine myelin oligodendrocyte glycoprotein (MOG) 35–55 peptide (MOG35–55, MEVGWYRSPFSRVVHLYRNGK) was synthesized with >99% purity (SciLight Biotechnology, China). C57BL/6 mice were injected with 200 μg MOG35–55 emulsified in 100 μg of complete Freund’s adjuvant (CFA, Sigma) and an additional 400 μg Mycobacterium tuberculosis H37RA (BD Biosciences) by subcutaneous injection into the flanks. These mice were also given 400 ng pertussis toxin (List Biological Laboratories) in 100 μl phosphate buffered saline (PBS) on the same day and 2 days later by intraperitoneal injection.

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… In Experiments 4 and 5, for visualization of CTb, all section sets were preincubated in 0.02 M PBS containing 3% normal goat serum, 0.2% Triton X-100 and a polyclonal rabbit anti-CTb primary antibody (List Biological Laboratories, USA) diluted to 1:40,000 …

• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

… Pertussis toxin was purchased from List Biological Laboratories, Inc. (Campbell, CA, USA) …

EAE induction and pharmaceutical treatment

To induce EAE disease, C57BL/6 male mice (6–8 weeks) received s.c. injections of complete Freund’s adjuvant (CFA) containing Mtb (200 μg) and MOG35–55 (100 μg) at 0 days post immunization (dpi). Pertussis toxin (200 ng per mouse) was administered through intraperitoneal (i.p.) injection at 0 and 2 dpi. GA or sGA (1 mg kg−1, 10 mg kg−1, or 100 mg kg−1) were administered through i.p. injection at 2 dpi (one-time-dosing) or 2 and 5 dpi (two-time-dosing) (n = 4 to 8). …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Chemicals and reagents

… Toxins (product numbers) purchased from List Biological Laboratories were: EF (178A), diphtheria toxin (150), Clostridium septicum alpha toxin (116L), C. perfringens epsilon toxin (126A), C. difficile toxins A (152C), B (155B), and binary toxin subunit A(157A), Pasteurella toxin (156), pertussis toxin (181), botulinum neurotoxins A (612A) and B (622A) (heavy chains), and cholera toxin (100B). …

• Product #178A – Anthrax Edema Factor (EF), Recombinant from B. anthracis
• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae
• Product #152C – Toxin A from Clostridium difficile
• Product #155 – Toxin B from Clostridium difficile
• Product #156 – Pasteurella multocida Toxin
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #612A – Botulinum Neurotoxin Type A Heavy Chain, Recombinant, Binding Domain
• Product #622A – Botulinum Neurotoxin Type B Heavy Chain, Recombinant, Binding Domain
• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

… On the day of and 2 d after immunization, 200 ng of pertussis toxin (List Biological) was injected intravenously …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Experimental autoimmune encephalomyelitis

Experimental autoimmune encephalomyelitis was induced using methods described previously (Lu et al., 2020). Specifically, complete Freund’s Adjuvant (CFA) was generated by adding heat killed Mycobacterium tuberculosis H37 RA (MT; Difco, No. 231141) with incomplete Freund’s adjuvant (Difco, No. 263910) to achieve a final concentration of 5 ​mg/ml. Equal volumes of MOG35-55 (Anaspec Inc.; 4 ​mg/ml) and the adjuvant mix were vortexed for 45 ​min. Mice were given four subcutaneous injections of MOG35-55/adjuvant emulsion into the hind flanks. Finally, mice received an i.p. injection of sterile PBS containing 400 ​ng of pertussis toxin (List Biological Laboratories) on days 0 and again on day 2 post immunization. Disease was scored by raters blinded to genotype as described previously (Lu et al., 2020). Control (CFA) C57BL/6 mice were immunized with CFA in the absence of antigen.

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)