Bacterial Toxin Research Citations

Animals and induction of disease

… Experimental autoimmune encephalomyelitis was induced with 50 μg myelin oligodendrocyte glycoprotein (MOG35-55; Synpeptide, Shanghai, China), 1 mg/ml complete Freund’s adjuvant (CFA; Sigma Aldrich, Oakville, Ontario, Canada) and 100–600 ng of pertussis toxin (PTX) for behavioral work (lot #180235A1A, CHO cell assay 0.03 ng/ml; List Biological Laboratories, Campbell, CA). A second lot of PTX was also used for molecular biology work, with PTX doses titrated to ensure similar clinical scores (80–180 ng, lot #180237A1, CHO cell assay 0.003 ng/ml), representing outcomes similar to “100–200 ng” from lot #180235A1A. …

• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer

… To deplete inflammatory monocytes, CCR2-DTR mice or their cohoused control littermates were injected intraperitoneally with 200 ng (10 ng/g body weight) of DT (List Biological Laboratories) as noted for a total of four doses …

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

Protective Antigen (PA) List Biological Laboratories, Inc., Campbell, USA

• Product #171E – Anthrax Protective Antigen (PA), Recombinant from B. anthracis

… Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free) List Biological Laboratories Cat# 181 …

• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)

Chemicals, Peptides, and Recombinant Proteins

Pertussis toxin List Biological Laboratories Cat# 180

EAE

Mice were immunized subcutaneously in the flanks with an emulsion containing MOG35–55 (80 μg/mouse) and M. tuberculosis H37Ra extract (3 mg/ml, Difco Laboratories) in CFA (100 μl/mouse). Pertussis toxin (100 ng/mouse, List Biological Laboratories) was administered intraperitoneally on days 0 and 2. Mice were monitored and assigned grades for clinical signs of EAE as previously described (Xiao et al., 2015). To evaluate the suppressive ability of Tim-1+ B cells, purified total B cells or Tim-1+ B cells were transferred i.v. into WT recipients. Hosts were then immunized to induce EAE.

• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer

… To deplete CCR2+ monocytes, 5g per kg DT (List Biological Laboratories) was injected into all mice at day 10, 13, 16 and 19 …

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

EAE induction and scoring

Mice were immunized subcutaneously at the tail base with 50 μg MOG35-55 peptide (GenScript) emulsified in complete Freund’s adjuvant (CFA, BD Biosciences) supplemented with 10 mg/ml Mycobacterium tuberculosis H37RA (BD Biosciences). In addition, mice were intraperitoneally injected with 100 ng of pertussis toxin (PTx) (List Biological Labs) on day 0 and day 2 post-immunization. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Toxin Neutralization Assay (TNA)
The functional ability of antibodies from vaccinated cattle and controls to neutralize anthrax lethal toxin was assessed using an in vitro TNA, as previously described by Ndumnego et al. [16]. The TNA was determined with J774A.1 mouse macrophage cells (ECACC cat no 91051511) exposed to anthrax lethal toxin in the presence of antibodies. Briefly, 1.0 × 105 J774A.1 macrophages per well in Dulbecco’s Modified Eagle Media (DMEM) and 10% foetal bovine serum (FBS) were incubated at 37 °C and 5% CO2 overnight in 96-well cell culture plates. Sera from each animal were 2-fold serially diluted in duplicates with the starting dilution of 1:50 in DMEM formulated with 5% FBS containing PA (500 ng/mL) and LF (400 ng/mL) (List Biological Laboratories Inc, Campbell, CA, USA) for 1 h at 37 °C and 5% CO2 before addition to the overnight-cultured confluent J774A. …

• Product #171E – Anthrax Protective Antigen (PA), Recombinant from B. anthracis
• Prodcut #172 – Anthrax Lethal Factor (LF), Recombinant from B. anthracis

Detection of anthrax-specific antibody levels

Anthrax-specific antibody levels against protective antigen were measured by ELISA12. High binding ELISA plates were coated with 100 ng per well of anthrax protective antigen (List Biological Laboratories Inc., Campbell, CA, USA) in carbonate buffer, pH 9.6 and incubated at 37 °C for 1 h, then overnight at 4 °C. …

• Product #171E – Anthrax Protective Antigen (PA), Recombinant from B. anthracis

… Seventy two hours following adenoviral treatment, cells were pretreated with 50uM Z?VAD?FMK (BD Biosciences) for 1 h prior to stimulation with 100 ng/ml recombinant human TNFa (Peprotech), 10ng/mL LPS (List Biological Laboratories, Campbell, CA, USA), …

Author did not specify which List Labs LPS product was utilized in their research.
List Labs provides the following LPS products: https://listlabs.com/product-information/lipopolysaccharides/