Bacterial Toxin Research Citations

EAE induction and clinical scoring

EAE was actively induced in 6- to 10-week old Sarm1 knockout mice and wild type littermates by subcutaneous injection of 200 μg of myelin oligodendrocyte glycoprotein peptide 35–55 (MOG35-55, Anaspec) emulsified in complete Freund’s adjuvant (CFA) containing 500 μg M. Tuberculosis H37 RA (Difco) and intraperitoneal injection of 300 ng pertussis toxin (List Biological Laboratories), followed by a second intraperitoneal injection of 300 ng pertussis toxin 2 days later. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Mouse study design

Male C57Bl/6 mice or Saa3−/− mice on the C57Bl/6 background (described in7) between 2–4 months of age were injected with either LPS (intraperitoneal, 0.25, 2.5, or 25 µg, E Coli 0111:B4, List Biological Laboratory), silver nitrate (AgNO3, subcutaneous, 0.5 mL of a 1% solution, Sigma), or casein (subcutaneous, 0.5 mL of a 5% solution) (n = 3 mice/group). …

• Product #201 – LPS from Escherichia coli O111:B4

In Vitro Stimulation Assay.

… For the NFκB activity assay, Hox-derived macrophages were transduced with lentivirus carrying pHAGE NFκB-TA-LUC-UBC-GFP-W (a gift from Darrell Kotton (Boston University, Boston, MA); Addgene plasmid #49343) (69). Lipopolysaccharide (LPS) (Salmonella minnesota R595, List Biological Laboratories) (10 µg/mL) and 14,15-diHETrE (Cayman Chemical) (10 µM) were used to stimulate transfected macrophages for 6 h. Luciferase activity was measured using Nano-Glo luciferase assay as described by the manufacturer (Promega).

• Product #304 – LPS from Salmonella minnesota R595 (Re)

… For ablation of cDC1 studies, WT and XCR1DTR were injected i.p., with 25 ng diphtheria toxin (DT) (150, List Biological Lab) per gram of body weight on day 6 post tumour engraftment …

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

… were cultured in complete DMEM-F-12 medium (Gibco) with 5% horse serum (Gibco), 10 g-ml Insulin (Sigma-Aldrich), 20 ng-ml Epidermal Growth Factor (Sigma-Aldrich), 0.5 g-ml Hydrocortisone (Sigma-Aldrich,), and 100 ng-ml Cholera toxin (List Biological …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

Materials and Methods

1.1 “Main reagents and instruments” Diphtheria toxin (diphtheria toxin, DT, List Biological Laboratories, USA); …

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

Neutrophil Recruitment Models

Lipopolysaccharide (LPS) challenge: Intratracheal instillation of LPS from E. coli (LIST Biologicals Campbell, CA) into lungs of Zfp30+/+ and Zfp30-/- mice was carried out at a dose of 0.3 mg per kg of body weight using previously described methods (Limjunyawong et al. 2015, Mock et al. 2020). …

Author did not specify which List Labs LPS product was utilized in their research.
List Labs provides the following LPS products: https://listlabs.com/product-information/lipopolysaccharides/

… Pertussis toxin (200 ng; List Biological Laboratories) in PBS was administered ip on days 0 and 2. One group of mice received adjuvant and pertussis toxin only and served as the control. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… To deplete Tregs, diphtheria toxin (DT) (List Biologicals Laboratories, Campbell, CA) was given intraperitoneally to Foxp3DTR or Foxp3EGFP mice before and after LPS …

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

… The cytotoxic titer was defined as the reciprocal of the highest dilution that produced rounding in 80% of Vero cells for each sample. Vero cells treated with purified C. difficile toxins (A and B) and antitoxin (List Biological Labs, 152C and 155C; TechLabs, T5000) were used as controls …

• Product #152C – Toxin A from Clostridium difficile
• Product #155 – Toxin B from Clostridium difficile