Citations

4.2.9 Animal model

4.2.9.1 EAE

EAE was induced as described before (Zinser et al., 2004). In brief, on day 0, 10-12 week old female C57BL/6N mice were subcutaneously immunized with a 1:1 ratio of MOG peptide35-55 (50 µg; Charité, Berlin, Germany) and complete Freund`s adjuvant (CFA; Sigma-Aldrich) enriched with 10 µg/ml Mycobacterium tuberculosis (H37Ra; Difco/BD Biosciences). Furthermore, pertussis toxin (200 ng; List Biological Laboratories, Campbell, California, USA) was injected intraperitoneally on days 0 and 2.

Sensitization of mice.

Mice received 1 mg of cashew protein extract supplemented with 10 μg of Cholera Toxin (List Biological Laboratories) per intragastric route, at a rate of one intragastric administration per week for 6 consecutive weeks.

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

… LPS (E. coli O11: B4-derived) was purchased from List Biologicals (Campbell, CA, USA), …

• Product #201 – LPS from Escherichia coli O111:B4

… Mice also received two intraperitoneal injections of 350 ng of pertussis toxin (List biological laboratories, Inc) at the time of induction and 48 hours later …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

3.2. EAE induction

… After MOG35-55 injection, mice received varying amounts (as listed) of Bordetella pertussis toxin (PTX) in glycerol buffer depending upon the experiment,
intraperitoneally (List Biological Laboratories, Campbell, CA; provided with Hooke Kit™ for EAE Induction) on day 0 and on day 1. …

• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… On experimental days 0, 7, 14, 21, and 28, mice (n= 8/group) were orally sensitized, by using a blunt needle, to the hen’s egg protein OVA (20 mg/0.5 mL PBS; grade V; Sigma- Aldrich) using cholera toxin (CT; 15 µg/0.5 mL; List Biological Laboratories …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

Phenotypes of toxin-receptor-gene knockouts by AncBE4max

sgRNAs targeting ANTXR1 and HBEGF were lentiviral infected into HeLa cells. GFP+ cells were FACS sorted and treated with PA/LFnDTA (70 ng/ml PA plus 50 ng/ml LFnDTA) for 48 hours or 7.5 ng/ml Diphtheria toxin (DT, List Biological Laboratories Inc.) for 60 hours and conducted in triplicates with individual treatment. …

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

… Unnicked diphtheria toxin (List Biologicals) was reconstituted at 1mg/1 mL in PBS and aliquoted and stored at -80C. Mice were injected i.p. at a dose of 40 ng/g two days prior to secondary challenge, and administered i.n. 50L (12ng/L) one day prior to secondary challenge …

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

Histology and microscopy

For vermal Purkinje cell retrograde tracing, goat ati-cholera toxin B subunit primary antibody (1:15000, List labs, 703) …

• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

… Immune system reactivity was supported by intraperitoneal administration of 0.6 μg or 1.2 μg PT (List Laboratories, Campbell, California, USA) dispersed in PBS (phosphate buffer saline) …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)