Citations

The EAE model was performed as previously described. In brief, each mouse was injected subcutaneously (s.c.) with 115 g MOG35-55 peptide (Washington Biotech) emulsified in CFA (263810, Difco) plus an intraperitoneal (i.p.) injection of 300 ng pertussis toxin (NC9675592, List Biological) on days 0 and 2. Clinical signs were assessed daily as described …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Induction of EAE

On day 0, C56BL/6 mice received a subcutaneous injection in both rear flanks containing 200 µg of MOG35-55 peptide (MOG) (Amino acid sequence: M−E−V−G−W−Y−R−S−P−F−S−R−VV−H−L−Y−R−N−G−K; Synpeptide, Shanghai, CHN) and Complete Freund’s adjuvant (CFA) (Difco, Detroit, MI, USA), which contains 500 µg of desiccated non-viable M. Turberculosis (Difco, Detroit, MI, USA). Simultaneously, they received an intraperitoneal injection of 200 ng of Pertussis Toxin (PT) (List Biologic, Campbell, CA, USA). Mice received a second dose of PT two days later.

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Immunofluorescence staining

For immunofluorescence staining, 30-µm-thick frozen sections were incubated with the following primary antibodies: rabbit anti-serotonin (5-HT, 1:2000, Sigma #S5545), rabbit anti-TH (1:500, Millipore #AB152), rabbit anti-tropomyosin receptor kinase C (TrkC, 1:500, Abcam ##ab43078), and mouse anti-synaptophysin (Syn, 1:1000, Millipore #MAB5258), rabbit anti-glia fibrillary acidic protein (GFAP, 1:1000, Abcam, #ab7260) and goat anti-cholera toxin B (CTB, 1:2000, List Biological Laboratories, #703). Signal was detected with the corresponding second antibodies conjugated to Alexa Fluor 405 or 488 or 594 fluorescence (1:1000, Invitrogen).

• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

… At 0 and 24 h after immunization, mice were intraperitoneally injected with pertussis toxin (List Biological, Campbell, CA). For adoptive transfer, unless stated otherwise, recipient mice were injected via the caudal vein with 5 106 MOG3555- specific CD8+ or CD4+ enriched T cells prepared as described previously in 0.2 mL phosphate buffer saline (PBS). …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EAE induction. Active EAE was induced by subcutaneous (s.c.) injection of 100 μg MOG35-55 peptide (United Biochemical Research) in 50 μL of CFA containing 5 mg/mL Mycobacterium tuberculosis H37Ra (Difco Laboratories). Mice also received intraperitoneal injections of 200 ng Bordetella pertussis toxin (List Biological) on days 0 and 2. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… injection of live bacteria. To achieve this, endotoxemia was induced by intraperitoneal (ip) injection of LPS (Escherichia coli0111:B4, List Biologicals; 0.5 mg/kg) 4 h prior to infection with live bacteria. …

• Product #201 – LPS from Escherichia coli O111:B4

… phosphate buffered saline (PBS) with 15 µg cholera toxin (CT, List Biological Laboratories Inc., Campbell, CA, USA) per mouse). The sham-sensitised control group received CT in PBS alone. …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

… Intraperitoneal injections of 250 ng pertussis toxin (List Biological Laboratories Inc.) were administered immediately following MOG3555 and 48 h after. EAE severity was scored on a 0 to 5 scale reflecting degree of limb paralysis and motor dysfunction per Hooke Laboratories protocol …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Induction of EAE and clinical score

The MOG35-55 (MEVGWYRSPFSRVVHLYRNGK) peptide was purchased from Polypeptide Laboratories (San Diego, CA, USA). Mice were immunized subcutaneously with 50 µg of MOG35-55 peptide
emulsified in complete Freund’s adjuvant (CFA, BD Difco, Franklin Lakes, NJ, USA) containing 500 µg of killed Mycobacterium tuberculosis (Strain H37a, Difco). Mice were injected intravenously with 200 ng of pertussis toxin (List Biological Laboratories, Campbell, CA, USA) at days 0 and 2 postimmunization. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae List Biological Laboratories, INC. 103B …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt