Bacterial Toxin Research Citations

Diet and treatment

Breeding cages were randomly assigned to one of the experimental isocaloric diets (AIN-93G, BioServ): standard (Stand, BioServ #S7628), n-3 long chain (LC)-PUFA enriched (n-3, BioServ #S7630; soybean oil replaced with Menhaden Fish oil) or n-6 LC-PUFA supplemented (n-6, BioServ #S7629; based on corn oil). The detailed composition of the diets is presented in our previous publication [14]. The day of birth was defined as postnatal day (P) 0. At P9, pups received a single intraperitoneal injection of lipopolysaccharide (LPS from E. coli O55:B5, 1 mg/kg, Cat# 423, List labs) or equivalent volume of saline. The dams remained on the experimental diet until offspring sacrifice (P10-P12). To allocate pups to particular experimental groups (LPS or saline), randomization in matched pairs was performed based on sex and weight of animals [15]. Number of animals in each group are shown in Suppl. Table 1.

• Product #423 – ULTRA PURE LPS from Escherichia coli O55:B5

… depletion was performed in CD169DTR/+ mice upon i.p. injection with 10 µg/kg DT (List Biological, Lot #15043A1) in 100 µL PBS. …

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

… pertussis and/or given 100 ng purified PT (List Laboratories) in 20 µl of phosphate-buffered saline (PBS) twice 4 weeks apart. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… Pertussis, Tetanus, and Diphtheria antigens (PT, PRN, Fim2/3, TT, DT (all from List Biological Laboratories), …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

• Product #187 – Pertactin from B. pertussis (69 kDa Protein)
• Product #186 – Fimbriae 2/3 from B. pertussis
• Product #190 – Tetanus Toxin from Clostridium tetani
• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

… Intraperitoneal injection of pertussis toxin (300 ng per 200 µl; 180, List Biological Laboratories) was performed days 0 and 2 after MOG immunization. Mice were monitored and scored daily on a scale of 0-15. EAE mice during chronic disease (day 45) were euthanized …

• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer

… After immunization, 0.25 µg of pertussis toxin (PTX) (List Biological Laboratories, Campbell, California, USA) dissolved in phosphate-buffered saline (PBS) was injected intraperitoneally on day 0 and day 2 …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Stereotaxic surgery

… For retrograde tracing of TRN-projecting and PVT-projecting PL cells CTB-488 and CTB-555 (1.0% in PBS, Thermo Fisher Scientific) were injected into the TRN (0.5 µL) and PVT (0.6 µL), respectively, and allowed 4 d for retrograde transport. For retrograde tracing of PVT-projecting TRN cells unconjugated CTB (List Labs Product No. 104) was injected into the PVT (0.6 µL). For retrograde labeling of PVT-projecting TRN cells for RNAscope, retrobeads (0.6 µL, LumaFluor, Inc.) were injected into the PVT and allowed 7 d for retrograde transport. …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

EAE induction

The female C57BL/6 mice (12 weeks old) used for the induction of EAE were purchased from KOATECH (Pyeongtaek, Gyeonggi, Republic of Korea). All mice were housed and maintained in a pathogen-free facility at Seoul National University (SNU) College of Medicine. …To induce EAE, mice received bilateral subcutaneous injections of a total of 200 μg of MOG 35–55 peptide (GeneScript, Piscataway, NJ) emulsified in CFA (BD Biosciences, San Jose, CA), followed by i.p. injection of 250 ng pertussis toxin (PTX) (List Biological Labs, Campbell, CA) 4 and 24 h later. The clinical scores of EAE mice were assessed daily.

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Antigens

Overlapping peptide pools targeting ESAT6 and CFP10 were used to stimulate T cells for these studies (BEI Resources). Peptides were 15 or 16 mers with 11 or 12 amino acid overlaps for ESAT6 protein and 11 amino acid overlaps for CFP10. Mtb whole-cell lysate from H37Rv was also used to stimulate T cells (BEI Resources). Dimethyl sulfoxide (DMSO) (Sigma-Aldrich) was used as a negative control. Staphylococcus enterotoxin B (List Biological Laboratories) was used as a positive control for the low-exposure cohort.

… Pertussis toxin (100ng per mouse; List Biological Laboratories) was injected intravenously on days 0 and 2 after immunization. The course of EAE disease was monitored daily, and each mouse was assigned a clinical score according to the following criteria: …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)