Citations

… Cholera toxin (List Biological Labs, Campbell, CA) …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

… Cholera Toxin (Azide-Free) from Vibrio cholerae Inaba 569B List Biological Laboratories, INC Cat#100B …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

EAE introduction and evaluation

Each mouse was injected subcutaneously with a 0.2 mL emulsifier containing 50% MOG35 − 55 (Genescript, USA) and 50% complete Freund’s adjuvant (CFA; Sigma‒Aldrich, USA) at the posterior neck and root of the tail on day 0. The emulsifier was prepared from 200 µg of MOG35 − 55 dissolved in 0.1 mL of PBS with an additional 4 mg/mL Mycobacterium tuberculosis H37Ra (Sigma‒Aldrich, USA). At the same time, 300 ng of PTX (List Biological Laboratories 181, USA) was injected intraperitoneally on days 0 and 2. …

• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)

Intranasal immunization

BALB/c mice were anesthetized with isoflurane (Pfizer Inc., New York, NY, USA) and immunized intranasally on days 0, 7, 14, and 21 with 100 µg of OVA (Sigma-Aldrich) in PBS together with 0.5 µg of cholera toxin (CT; List Biological Laboratories Inc., Campbell, CA, USA), which is used as a mucosal adjuvant in a total volume of 13 µL per mouse. …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

… emulsified in Complete Freund’s Adjuvant (CFA, Sigma-Aldrich) followed by pertussis toxin (List Biological) on days 0 and 2 to induce …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… Adjuvant, complete H37 Ra VWR Cat#231131 Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free) List Biological Laboratories, Inc. Cat#181 …

• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)

Active EAE

Active EAE (aEAE) was induced in 8–12 weeks old female VE-cadherin-GFP knock-in mice in C57BL/6 J background using the MOGaa35–55-peptide using a protocol established before89,90. Briefly, 200 μg of MOGaa35-55-peptide in Complete Freund’s Adjuvant (CFA, LabForce; Santa Cruz Biotechnology) supplemented with 4 mg/ml nonviable, desiccated Mycobacterium tuberculosis (H37RA; Difco/BD Biosciences/BD Clontech) were injected subcutaneously on day 0. Pertussis toxin (List Biological Laboratories, Campbell, US) (300 ng in 100 μl PBS/mouse) was applied intraperitoneal (i.p.) on day 0 (immunization day) and day 2 post-immunization. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… a Co-injections with 0.2% beta subunit of cholera toxin. Source: List Laboratories …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

… (0.5 U/mL, U is the toxin amount that causes 50% of mice death within 4 days) BoNT type A1 from Clostridium botulinum (List labs) was mixed with …

… Pertussis toxin (List Biological Laboratories Inc., CA, USA) was intraperitoneally injected at 400 ng on days 0 and 2. After 15-20 days, mice displayed …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)