Bacterial Toxin Research Citations

… Goat anti-CTB List biological laboratories Cat# 703B …

• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

Blood cultures for cytokine production

Whole blood treated with heparin was used to set up whole blood cultures in 96-well plates. The external wells contained 200 mL sterile PBS to prevent excessive evaporation of experimental wells during the culture. Blood was diluted 10-fold in RPMI 1640 cell-culture medium(Gibco) and then 200μL/well was treated with 1)3μg/mL plate-coatedanti-CD3 antibody plus anti-CD28 antibody in solution (eBioscience)to stimulate T-cell cytokine production or 2) the same volume of isotype control antibodies (eBioscience). Undiluted blood (final blood dilution 1:2) was treated with 1) LPS from Escherichia coli 0111:B4 (List Biological Laboratories) at a final concentration of 20μg/mL …

• Product #201 – LPS from Escherichia coli O111:B4

… Pertussis toxin from List biological (USA), …

EAE induction, clinical scoring, treatment, histological analysis and ELISA

… EAE was induced in female C57BL/6 mice (10–12 week old) using 200 µg of myelin oligodendrocyte glycoprotein (MOG) peptide [sequence 35–55; (MEVGWYRSPFSRVVHLYRNGK)] emulsified in complete Freund’s adjuvant supplemented with heat-inactivated Mycobacterium tuberculosis H37RA (4 mg/ml) subcutaneously. 200 ng pertussis toxin was given i.p. at day 0 and 2 post immunizations. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… On day 353, mice in groups 1, 2, 7, 8 and 9 were challenged with 10ng tetanus toxin (List Biological Labs; kindly provided by the US Food & Drug Administration) by subcutaneous injection in the leg …

• Product #190 – Tetanus Toxin from Clostridium tetani

Experimental animal procedures

… Whey‐sensitized mice were sensitized orally via gavage with 20 mg of homogenized whey (WPC60; Milei, Friesland Campina) in 500 µL phosphate‐buffered saline (PBS), containing 10 µg cholera toxin (CT) (List Biological Laboratories) as an adjuvant.33 Sham‐sensitized mice received 10 µg CT in 500 µL PBS. Mice were sensitized once a week for 5 consecutive weeks, starting at day 14 (Figure 1). …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

Pharmacological Drugs

… Innate and inflammatory stimuli were used at the indicated concentrations: … LPS (List Biological Laboratories, INC, 100 ng/ml).

Author did not specify which List Labs LPS product was utilized in their research.
List Labs provides the following LPS products: https://listlabs.com/product-information/lipopolysaccharides/

Active induction of EAE

Active EAE was induced by immunization with the myelin oligodendrocyte glycoprotein (MOG) peptide 35–55 (MEVGWYRSPFSRVVHLYRNGK). 100 μg MOG35–55 peptide was emulsified in complete Freund’s adjuvant supplemented with M. tuberculosis extract H37Ra (Difco) and injected subcutaneously. Mice received 150 ng pertussis toxin (List Biological Laboratories) intraperitoneally on days 0 and 2.  …

Author did not specify which List Labs Pertussis Toxin was utilized.  List Labs provides the following Pertussis Toxin products:

Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Adenoid cell culture as activation-induced marker (AIM) assay

AIM assays were modified based on Ref. [28]. In brief, cryopreserved adenoid suspension cells prepared as “HLAC” were thawed, and then cultured in 96U-well plates with 1.4 × 106 cells/well in 200 µl CMT medium unless stated otherwise. Cells were either left unstimulated, or were stimulated with Pertussis Toxin mutant (PT; enzymatically inactive point mutant, highly purified and low endotoxin-tested, List Biological Laboratories via Biotrend) at 0.1, 1 or 5 µg/ml final concentration. As a control, cells were stimulated with the superantigen Cytostim (Miltenyi Biotec). Cells were cultured for 18–19 h in the AIM assay and subsequently, AIM markers PD-L1, CD25 and OX40 were measured on non-Treg T cells by flow cytometry. For details and variations of the AIM assay, as well as antibody panels, see Supplemental materials and methods Sections 3–6.

• Product #184 – Pertussis Toxin Mutant

… After another rinse they were transferred to a mixture of two primary antibodies, polyclonal goat anti-CTB (List Biological Laboratories, product #703, 1:7500) …

• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

… Clostridium difficile Toxin B (100 ng/mL; List Biologicals), …

• Product #155 – Toxin B from Clostridium difficile