Bacterial Toxin Research Citations

Animals and Regents

… Pertussis toxin (PTX) was purchased from List Biological Lab …

Induction and assessment of EAE

EAE was induced as previously described [19]. Mice received a subcutaneous injection in the flank of 200 μg MOG35–55 peptide emulsified in CFA containing 500 μg Mycobacterium tuberculosis H37RA. Immediately thereafter and again 48 h later, the mice were given an intraperitoneal injection of 300 ng PTX in 100 μl phosphate-buffered saline (PBS). …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Immunization

Eight micrograms of OVA (Sigma) and 2 μg of cholera toxin subunit B (List Biological) in 6 μL of sterile PBS or 6 μL of PBS were administered intranasally. Mice were sacrificed 7 days later and parotid glands were removed and frozen. For detection of CD3+ cells, representative sections were prepared for fluorescent immunohistochemistry. The number of CD3+ cells was counted in 15 high power fields (hpf).

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

… After 72 h of puromycin selection, cells were collected and divided into two groups (2.8×107 cells/group) and cultured for 2 weeks in either the presence (resistant) or absence (control) of 2 ng/ml Shiga toxin (List Biological Laboratories) …

Author did not specify which List Labs Shiga Toxin was utilized for their research.
List Labs offers the follows:

https://listlabs.com/product-information/shiga-toxins/

… B. anthracis lethal factor (LF) was acquired from List Biologicals. …

• Product #172 – Anthrax Lethal Factor (LF), Recombinant from B. anthracis

EAE induction

To induce EAE disease, complete Freund’s adjuvant, CFA (#F5881, Sigma) containing 400 μg Mycobacterium tuberculosis, Mtb (#DF3114-33-8, Fisher), and 100 μg myelin oligodendrocyte glycoprotein 35–55 peptide (MOG35–55, United Peptides) were subcutaneously administered at 0 and 7 days post-induction (dpi). Pertussis toxin (200 ng/mouse) (#181, List Biological Laboratories, Inc.) was administered on days 0, 2, and 7 dpi. …

• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)

… Pertussis toxin List Biological Laboratories Cat # LB181 …

• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)

Reagents

… Lipopolysaccharide (E. coli, serotype O111:B4 L2630) was from List Biological Laboratories (Campbell, CA) …

Alcohol Exposure and LPS Challenge

Mice were exposed to ethanol (2 or 4 g/kg), propanol (2 g/kg), isopropanol (2 g/kg) or PBS via intraperitoneal injection. One hour later, a lethal dose of LPS (10 mg/kg, i.p.) was injected. Animals were monitored every 2 h for 36 h post LPS injection.

• Product #201 – LPS from Escherichia coli O111:B4

Immunization

Eight micrograms of OVA (Sigma) and 2 µg of cholera toxin subunit B (List Biological) in 6 µL of sterile PBS or 6 µL of PBS were administered intranasally. Mice were sacrificed 7 days later and parotid glands were removed and frozen …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

Chemicals and cell culture

… cholera toxin (10 μg/ml; List Biological Laboratories, Inc., Campbell, CA, USA), …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

… All mice then received a single intraperitoneal injection of pertussis toxin (List Biological Laboratories, #180) at a concentration of 2 ng/l in 200 l PBS …

• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer