Bacterial Toxin Research Citations

Immunization

Eight micrograms of OVA (Sigma) and 2 μg of cholera toxin subunit B (List Biological) in 6 μL of sterile PBS or 6 μL of PBS were administered intranasally. Mice were sacrificed 7 days later and parotid glands were removed and frozen. For detection of CD3+ cells, representative sections were prepared for fluorescent immunohistochemistry. The number of CD3+ cells was counted in 15 high power fields (hpf).

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

… After 72 h of puromycin selection, cells were collected and divided into two groups (2.8×107 cells/group) and cultured for 2 weeks in either the presence (resistant) or absence (control) of 2 ng/ml Shiga toxin (List Biological Laboratories) …

Author did not specify which List Labs Shiga Toxin was utilized for their research.
List Labs offers the follows:

https://listlabs.com/product-information/shiga-toxins/

… B. anthracis lethal factor (LF) was acquired from List Biologicals. …

• Product #172 – Anthrax Lethal Factor (LF), Recombinant from B. anthracis

EAE induction

To induce EAE disease, complete Freund’s adjuvant, CFA (#F5881, Sigma) containing 400 μg Mycobacterium tuberculosis, Mtb (#DF3114-33-8, Fisher), and 100 μg myelin oligodendrocyte glycoprotein 35–55 peptide (MOG35–55, United Peptides) were subcutaneously administered at 0 and 7 days post-induction (dpi). Pertussis toxin (200 ng/mouse) (#181, List Biological Laboratories, Inc.) was administered on days 0, 2, and 7 dpi. …

• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)

… Pertussis toxin List Biological Laboratories Cat # LB181 …

• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)

Reagents

… Lipopolysaccharide (E. coli, serotype O111:B4 L2630) was from List Biological Laboratories (Campbell, CA) …

Alcohol Exposure and LPS Challenge

Mice were exposed to ethanol (2 or 4 g/kg), propanol (2 g/kg), isopropanol (2 g/kg) or PBS via intraperitoneal injection. One hour later, a lethal dose of LPS (10 mg/kg, i.p.) was injected. Animals were monitored every 2 h for 36 h post LPS injection.

• Product #201 – LPS from Escherichia coli O111:B4

Immunization

Eight micrograms of OVA (Sigma) and 2 µg of cholera toxin subunit B (List Biological) in 6 µL of sterile PBS or 6 µL of PBS were administered intranasally. Mice were sacrificed 7 days later and parotid glands were removed and frozen …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

Chemicals and cell culture

… cholera toxin (10 μg/ml; List Biological Laboratories, Inc., Campbell, CA, USA), …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

… All mice then received a single intraperitoneal injection of pertussis toxin (List Biological Laboratories, #180) at a concentration of 2 ng/l in 200 l PBS …

• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer

Materials and Methods

Anthrax lethal factor, Product #169 and MAPKKide® Plus, Prod #532 are from List Biological Laboratories,
Inc. The 96-well, black, flat bottom, nonbinding plates used for the fluorescent plate assay were from
Corning (cat # 3991). The filtered adult bovine plasmas: Na-Citrate, cat #7310806; K2-EDTA, cat #7310807;
K3-EDTA, cat #7310808; Li-Heparin, cat #7310810 ; Na-Heparin, cat #7310811; and the filtered adult bovine
serum cat #7330800 were purchased from Lampire Biological Laboratories.

Sample Preparation: Stock solutions of the fluorogenic substrate, MAPKKide® Plus, were made 1.25 mM
in DMSO. The substrate was then diluted 5-fold to 250 µM in assay buffer: 20 mM HEPES, pH 8.0 containing
0.1% Tween-20.

LF Activity Assay: LF was spiked into each plasma or serum sample and then each was diluted 1:5 using
assay buffer. The reaction was initiated by addition of 10 μM MAPKKide® Plus directly to the 1:5 diluted
plasma or serum, and the assays were run at 37°C using the kinetic mode of the plate reader with readings
at 1-minute intervals. The samples were run in triplicate with 9 replicate blanks. The limit of detection was
calculated from the normal distribution of the blank samples (mean + 3 stdev; n = 3 triplicates).

• Product #169L – Anthrax Lethal Factor (LF-A), Recombinant from B. anthracis Native Sequence
• Product #532 – MAPKKide® Plus (AMC) Specific Substrate for Anthrax Lethal Factor