Bacterial Toxin Research Citations

Mouse Primary Corneal Epithelial Cells (MPCEC):

… Primary corneal epithelial cell cultures were established using a modification of the established explant culture method.^38,39 Briefly, eyes were enucleated, and the cornea was washed with Ca⁺⁺-free PBS (pH 7.2). Each cornea was cut in half and placed in a 35-mm dish (Fisher Scientific) with the epithelial side up. One and half milliliters of DMEM with 10% serum containing 40 g/mL gentamicin, 1% ITS, and 100 ng/mL cholera toxin (LIST Biological Laboratories, Inc., Campbell, CA, USA) was added and the tissue was cultured in a humidified incubator at 37C with 5% CO2. Culture medium was replaced every 2 days. Cells were passged using 0.25% trypsin (Fisher Scientific), and subculturing in DMEM with 3% serum containing 40 g/mL gentamicin, 1% ITS, and 100 ng/mL cholera toxin. 

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

Cells and Reagents:

… Salmonella minnesota ultra pure lipopolysaccharide (LPS) was from List Biologicals Laboratories (Campbell, CA), …

• Product #434 – ULTRA PURE LPS from Salmonella minnesota R595 (Re)

… Cells were placed into six-well plates for stimulation with 10ngml1 Salmonella minnesota R595 (Re) ultra-pure LPS (List Biological) for different periods of time. …

• Product #434 – ULTRA PURE LPS from Salmonella minnesota R595 (Re)

EAE:

… Pertussis toxin (200 ng per mouse; no. 181, List Biological Laboratories) was administered intravenously 0 and 2 days after induction. Clinical evaluation was performed daily using a five-point scale: 0, no clinical signs; 1, limp tail; …

• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)

EAE Induction:

Induction of EAE was performed as described earlier (33). Briefly, active EAE was induced by immunization with 200 µg MOG₃₅₅₅ peptide emulsified in complete Freund’s adjuvant (CFA, Sigma-Aldrich) containing 800 µg of heat-killed Mycobacterium tuberculosis (Difco Laboratories). The emulsion was administered s.c. as four 50µl injections into the flanks of each leg. In addition, 200 ng of pertussis toxin (List Biological Laboratories) dissolved in 200 µl PBS was injected i.p. on days 0 and 2 after immunization as described earlier (34). …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… Immediately after immunization and again 48 h later, the mice were received a tail vein injection of 200 ng of pertussis toxin (List Biological Laboratories, CA, USA) in 100 l of PBS (Zhang et al., 2017). …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… LPS (lipopolysaccharide; TLR4 agonist) List Biological Lab (San Jose, CA, USA), …

Author did not specify which List Labs LPS product was utilized in their research.
List Labs provides the following LPS products: https://listlabs.com/product-information/lipopolysaccharides/

… A total volume of 0.2 ml was injected sc into the flank at both right and left sides. On the same day and 48 h later, mice were injected ip with 150 ng in 0.2 ml of pertussis toxin (List Biological Laboratories)….

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… Cholera toxin was obtained from List Biological Laboratories (Campbell, CA). …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

Tissue clearing and nigral CTB tracing:

Lesion of the nigrostriatal pathway and VTA in Sprague-Dawley rats used for tissue clearing and light sheet microscopy or simultaneous rabies / CTB tracing received lesions was induced by unilateral injection of 6-hydroxydopamine with a volume of 3.0 L and 2.5 L at freebase concentration of 3.5 g/L to the following coordinates relative to bregma: A/P 4.4; M/L 1.2; D/V (from dura) 7.8; and A/P 4.0; M/L 0.8; D/V (from dura) 8.0, adjusted to flat head, respectively. Rats were grafted with 150,000 VM-patterned progenitors in two deposits to the striatum at a total volume of 2 L, at a concentration of 75,000 cells/L, at a rate of 1 L per minute and diffusion time of 2 min, to the following coordinates relative to bregma: A/P +0.5; M/L 2.6; D/V (from dura) 4.0/-3.5; adjusted to flat head. Animals grafted in the nigra received 75,000 cells in a total volume of 1 L to the following coordinates relative to bregma: A/P 5.2; M/L 2.3; D/V (from dura) 7.0; adjusted to flat head. For rabies / CTB tracing, animals first received two 500 nL nigral CTB (choleragenoid in low salt, List Biological Laboratories, 0.4% in PBS) injections at A/P 5.3; M/L 1.6, 2.6; D/V 7.2, 6.7 (from dura); adjusted to flat head relative to bregma, at a rate of 100 nL/min and a diffusion time of 3 minutes, followed by a total volume of 2 L G-rabies at a dilution of 5% of the stock injected at a rate of 0.5 L per minute and diffusion time of 2 min to the following coordinates relative to bregma: A/P +0.5; M/L 2.6; D/V (from dura) 4.0/-3.5; adjusted to flat head. Animals were perfused after 1 week of rabies / CTB tracing.

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

Primary antibodies were used as follows:

… Goat anti-CTB (1:2500; List Biological Laboratories); …

• Product #703 – Anti-Cholera Toxin B Subunit (Goat)