Bacterial Toxin Research Citations

… CTb, Goat, 703, 1:1000, List Biological. …

• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

… 50µl of the emulsion were injected in each axillary fossa. 200ng of pertussis toxin (LIST BIOLOGICAL LABORATORIES, INC) were administered intravenously at the time of immunization and 48 h later. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Cell culture:

MCF-10A cells were obtained from ATCC (Manassas, VA, USA) and were grown using Dulbeccos Modified Eagles Medium/Nutrient Mixture F-12 (DMEM/F12, 1:1; Invitrogen, Carlsbad, CA, USA) supplemented with 5% horse serum (Invitrogen), 20ng/ml recombinant human epidermal growth factor (EGF; R&D systems, Minneapolis, MN, USA), 0.5g/ml hydrocortisone (Sigma, St. Louis, MO, USA), 100ng/ml cholera toxin (List Biological Lab, St, Campbell, CA, USA), …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

– List Biological Laboratories Inc., Campbell, CA, USA; Cat# 703; goat, polyclonal; RRID: AB_10013220 1:80,000 Anti-PHA-L PHA-L (Phaseolus vulgaris leucoagglutinin) Choleragenoid, List Biological Laboratories Inc., Campbell, CA, USA) in distilled water …

• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

Author did not specify which CTB was utilized.  List Labs has provided Product #103B (Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae) and Product #104 (Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt).  The former has been discontinued.  The latter may be substituted for the former, if the researcher takes into account the difference in the buffer in which each product is provided and the amount of protein per vial.  When Product #103B was reconstituted with 200 l of water, it contained 1 mg of protein in 0.05 M Tris, 0.2 M NaCl, 0.001 M Na₂EDTA, 0.003 M NaN_3, pH 7.5.  When Product #104 is reconstituted with 0.25 ml water, it contains 0.5 mg of Cholera Toxin B Subunit (choleragenoid) in 0.01 M Sodium Phosphate at pH 7.5.

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

Induction and scoring of EAE:

<... Each animal also received 200ng pertussis toxin (List Biological, Campbell, CA, USA) in 0.1ml PBS by intraperitoneal injection at 0h and 48h post-immunization. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Active EAE induction:

… A booster immunization was applied subcutaneously on day 7 over contralateral lymph nodes. Pertussis toxin (500 ng/mouse) (List Biological Laboratories) was injected intraperitoneally on days 0 and 2 (54). …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… Wild-type and Plgf-KO mice on the C57BL/6N background were subcutaneously immunized with 200 µg of MOG3555 peptide (MEVGWYRSPFSRVVHLYRNGK) emulsified in 100l CFA (Sigma-Aldrich) in the abdominal flank. On days 0 and 2, the immunized mice received additional intraperitoneal injections of 200ng of pertussis toxin (List Biological Labs). …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… antiCTB at 1:10,000 (List Biological Laboratories, Campbell, CA). …

• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

… An anti-CTB (Goat polyclonal, Cat #703, Lot # 7032A9, 1:5000; List Biologicals) was used to label PhMNs. …

• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

Reagents and antibodies

… For cell culture experiments, Val-boroPro was resuspended in DMSO containing 0.1% TFA to prevent compound cyclization. Anthrax Lethal Factor (Catalog #172B) and PA (Catalog #171E) were purchased from List Labs, …

LDH cytotoxicity assays

For LDH release experiments involving primary macrophages, mBMDM and rBMDM were isolated from the femurs and tibias of 79-week-old male and female mice and 712-week-old male rats. 110⁷ cells were plated on 10cm Petri dishes. mBMDMs were differentiated in DMEM with 10% FBS and 20% L Cell medium for 6 days and rBMDMs in 30% L Cell medium for 79 days. Macrophages were scraped and reseeded at 0.2510⁶ cells/well in 12-well plates in Opti-MEM and incubated overnight before treatment. Cells were then treated as indicated with compounds or LT. …

In vitro and in cell LF cleavage assays

HEK 293T cells were seeded at 0.510⁶ cells/well in six-well plates and transiently transfected with 2g of the indicated plasmids using FuGENE HD. For the in vitro assay, lysates were harvested after 48h and normalized to 1mg/mL using the DC Protein Assay kit (Bio-Rad). These lysates were then incubated with LF (1g/mL) for the indicated time periods at 25C quenched by boiling with 2SDSPAGE sample buffer at the indicated times, separated by SDSPAGE, immunoblotted, and visualized using the Odyssey Imaging System (Li-Cor). For the in cell assay, after 48h after transfection cells were treated with LT (1g/mL, 6h) before lysates were harvested for immunoblotting as described above.

• Product #171E – Anthrax Protective Antigen (PA), Recombinant from B. anthracis
• Product #172 – Anthrax Lethal Factor (LF), Recombinant from B. anthracis