Bacterial Toxin Research Citations

… To induce EAE, adult female mice were injected subcutaneously in the flank/tail base with 200 µg MOG 3555 peptide emulsified in complete Freunds adjuvant (BD Biosciences) supplemented with 600µg Mycobacterium tuberculosis (strain H37Ra; BD Biosciences). Two i.p. injections of 400 ng pertussis toxin (List Biological Laboratories) were given 0 and 48 hours later. Clinical scores (0, healthy; 1, flaccid tail; 2, ataxia and/or paresis of hind limbs; 3, paralysis of hind limbs and/or paresis of forelimbs; 4, tetraparalysis; 5, moribund or death) were recorded daily …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Diphtheria– and tetanus-specific MBC detection by flow cytometry:

Protein biotinylation and formation of Agquantum dot (QD) complexes was performed using diphtheria toxin CRM197 (DT; List Biological Labs, CA), tetanus toxoid (TT; List Biological Labs), and human serum albumin (HSA; Sigma-Aldrich; used as a negative control) that were biotinylated using a ChromaLink Biotin Kit (Solulink, CA) according to the manufacturers instructions. …

• Product #149A – Diphtheria Toxin CRM₁₉₇, Mutant
• Product #191A – Tetanus Toxoid from Clostridium tetani

Food allergy model:

Peanut butter (3.75 mg; ~1 mg of protein; Kraft, Northfield, IL) with 10 µg of cholera toxin (List Biologicals, Campbell, CA) in 0.5 mL of PBS was administered intragastrically (Delvo SA, Biel, Switzerland) weekly for 4 weeks. …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

Antibodies:

Primary antibodies used in this study that are commercially available include: rabbit anti-MEK2 (Santa Cruz Biotechnology sc-523, AB_2281672), goat antiprotective antigen from Bacillus anthracis(List Biological Laboratories #771B), …

LFNEffector intoxication of T84 cells:

… For intoxication of cells, media was replaced over 105 cells previously seeded in a 12-well plate. 31.7 nM PA alone (List Labs, #171E) or in the presence of 13.6nM LFN, LFN-ACD, or LFN-RID was added to media and cells were incubated for 20 hr at 37C in the presence of 5% CO2. For co-intoxication of 13.6 nM LFNACD and LFN-RID, 3x excess PA was used to ensure equal translocation of both effectors.

• Product #171 – Anthrax Protective Antigen (PA), Recombinant from B. anthracis

… For the induction of MP4-EAE, mice received 200 µg MP4 (Alexion Pharmaceuticals Inc) emulsified in CFA and 200 ng pertussis toxin (List Biological Laboratories, #181, Lot #18123611) in 500 µl sterile phosphate-buffered saline (PBS) on the day of immunization …

• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)

… Targeted elimination of CX3CR1+ cells. CX3CR1-DTR mice were treated with diphtheria toxin (DT, List Biological Lab Inc., Campbell, CA) three times over seven days using a dose of 200ng/20g mouse. …

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

Drugs:

The following compounds were used in the reported studies: CL 316,241 (in saline; from Tocris); C75 (RPMI medium 1640; from Tocris);67,68 capsaicin (3.3% Tween-80 in saline; from Sigma)14, CNO (in saline; from Enzo Life Science)14, and diphtheria toxin (in saline; List Biological, cat. no. 150). All drugs were injected in a volume of 10 ml kg1 of body weight i.p. except for diphtheria toxin, which was injected at the dose of 50 g kg1 and the volume was 2.66 l g1 of body weight. When multiple injections were performed in the same experiment, the volume of each injection was adjusted to a total volume of 10 ml kg1 per animal.

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

Experimental Animal Procedures:

All mice were intragastrically (i.g.) sensitized to the cows milk protein whey (DMV International, Veghel, the Netherlands) dissolved in PBS (20 mg whey in 0.5 ml PBS, Lonza, Verviers, Belgium) with cholera toxin (CT, 15 µg CT in 0.5 ml, List Biological Laboratories Inc., Campbell, CA, USA) to induce food allergy or were sham-sensitized with PBS and CT alone on D0, 7, 14, 21, and 28 (Figure 1). …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

… Animals received 250 µg pertussis toxin (List Biological Laboratories) intraperitoneally (I.P.) on the day of immunization and 48 h later. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)