Bacterial Toxin Research Citations

4.1. Cell Line and Reagents for Development of the AP-1 Reporter Assay
Murine hepatoma Hepa1c1c7 cells (CRL-2026™) purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA) were cultured in an alpha minimum essential medium (MEM), supplemented with 10% FBS, 100 IU/mL penicillin, 100 µg/mL streptomycin, and 2 mM L-glutamine. Cultures were maintained at 37 °C in an incubator with 5% CO2. Lyophilized recombinant protective antigen (PA) and lethal factor (LF) were purchased from List Biological Laboratories, Inc. (Campbell, CA, USA), and reconstituted in sterile water to make stock solutions with a final concentration of 1 mg/mL for LF and 2 mg/mL for PA. The stock solutions were then diluted 10 times with 50% glycerol in sterile water to make stock solutions with a final concentration of 0.1 mg/mL for LF and 0.2 mg/mL for PA. …

• Product #171E – Anthrax Protective Antigen (PA), Recombinant from B. anthracis

Author did not specify which Lethal Factor (LF) was utilized; List Labs provides the following LF:

• Prodcut #172 – Anthrax Lethal Factor (LF), Recombinant from B. anthracis
• Product #169L – Anthrax Lethal Factor (LF-A), Recombinant from B. anthracis Native Sequence

Culture supernatant extract anthrax vaccine and its liquid and dry powder formulations preparation and characterization

… Total protein concentrations of the CSE vaccine were measured by bicinchoninic acid assay (BCA, Gene-Star, Beijing, China). An enzyme-linked immunosorbent assay (ELISA, Dakewe, Beijing, China) was used to quantify the PA protein in the CSE vaccine according to a previously method [Citation20]. Serially diluted commercial recombinant PA (rPA, 171E, List Labs, Campbell, CA, USA) was the reference standard. …

• Product #171E – Anthrax Protective Antigen (PA), Recombinant from B. anthracis

2.1.1. Experimental Materials

The following materials were used: anthrax protective antigen (PA), from List Labs in California, USA, stored at 4 °C; anti-protective antigen (Anti-PA) monoclonal antibody, from List Labs in California, USA, stored at 4 °C; …

… Recombinant, nicked PA63 from B. anthracis was obtained from List Biological Laboratories Inc., Campbell, CA. …

• Product #174 – Anthrax Protective Antigen, Activated (PA 63) from B. anthracis

… Anthrax protective antigen (PA) was obtained from List Biological Laboratories List Labs), and stored at 4°C. …

• Product #171E – Anthrax Protective Antigen (PA), Recombinant from B. anthracis

… Prediluted serum samples (1:50) were diluted by 2-fold in another 96-well plate and incubated with a constant amount of anthrax lethal toxin (100 ng/mL PA and 80 ng/mL LF; List Biological Laboratories, Campbell, CA, USA) for 30 min. The generated toxin-antiserum was then applied to J774A.1 cells and incubated for 4 h. …

• Product #171E – Anthrax Protective Antigen (PA), Recombinant from B. anthracis

Author did not specify which Lethal Factor (LF) was utilized; List Labs provides the following LF:

• Prodcut #172 – Anthrax Lethal Factor (LF), Recombinant from B. anthracis
• Product #169L – Anthrax Lethal Factor (LF-A), Recombinant from B. anthracis Native Sequence

… Goat anti-PA polyclonal antibody and recombinant PA were obtained from List Labs. …

• Product #171E – Anthrax Protective Antigen (PA), Recombinant from B. anthracis

Cell stimulation

… Bacillus anthracis Lethal Factor (0.5 mg mL -1) and Protective Antigen (0.5 mg mL -1) (Lethal toxin, List Labs, …

• Product #171E – Anthrax Protective Antigen (PA), Recombinant from B. anthracis

Author did not specify which Lethal Factor (LF) was utilized; List Labs provides the following LF:

• Prodcut #172 – Anthrax Lethal Factor (LF), Recombinant from B. anthracis
• Product #169L – Anthrax Lethal Factor (LF-A), Recombinant from B. anthracis Native Sequence

… Anthrax Protective Antigen List Biological Labs Cat# 171E Anthrax Lethal Factor List Biological Labs Cat# 172C …

• Product #171E – Anthrax Protective Antigen (PA), Recombinant from B. anthracis
• Prodcut #172 – Anthrax Lethal Factor (LF), Recombinant from B. anthracis

… Biotinylated recombinant PA83 (List Biological Labs, Campbell, CA) was bound …

• Product #171E – Anthrax Protective Antigen (PA), Recombinant from B. anthracis