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August 24, 2021
Proceedings Of The National Academy Of Sciences Of The United States Of America
Kim, E;Attia, Z;Woodfint, RM;Zeng, C;Kim, SH;Steiner, HE;Shukla, RK;Liyanage, NPM;Ghimire, S;Li, J;Renukaradhya, GJ;Satoskar, AR;Amer, AO;Liu, SL;Cormet-Boyaka, E;Boyaka, PN;
Product: Anthrax Protective Antigen (PA), Recombinant from B. anthracis
B. anthracis lethal toxin (LeTx) (i.e., PA plus B. anthracis lethal factor [LF] [List Biological]) was then added to the plates. After overnight incubation, MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide; Sigma-Aldrich] was added to assess the viability of macrophages as a function of redox potential …
• Product #171E – Anthrax Protective Antigen (PA), Recombinant from B. anthracis
Author did not specify which Lethal Factor (LF) was utilized; List Labs provides the following LF:
• Prodcut #172 – Anthrax Lethal Factor (LF), Recombinant from B. anthracis
• Product #169L – Anthrax Lethal Factor (LF-A), Recombinant from B. anthracis Native Sequence
August 1, 2021
Journal of Extracellular Vesicles
Budden, CF;Gearing, LJ;Kaiser, R;Standke, L;Hertzog, PJ;Latz, E;
Product: Anthrax Protective Antigen (PA), Recombinant from B. anthracis
Differentiation and stimulation of THP-1 monocytes
… 1µg/ml protective antigen (PA; 90 min; List Biological Laboratories …
• Product #171E – Anthrax Protective Antigen (PA), Recombinant from B. anthracis
July 14, 2021
Cell
Evavold, CL;Hafner-Bratkovi?, I;Devant, P;D'Andrea, JM;Ngwa, EM;BorÂi?, E;Doench, JG;LaFleur, MW;Sharpe, AH;Thiagarajah, JR;Kagan, JC;
Product: Anthrax Protective Antigen (PA), Recombinant from B. anthracis
Protective Antigen List Biological Labs #171E
• Product #171E – Anthrax Protective Antigen (PA), Recombinant from B. anthracis
July 4, 2021
ACS Infectious Diseases
Martchenko Shilman, M;Bartolo, G;Alameh, S;Peterson, JW;Lawrence, WS;Peel, JE;Sivasubramani, SK;Beasley, DWC;Cote, CK;Demons, ST;Halasahoris, SA;Miller, LL;Klimko, CP;Shoe, JL;Fetterer, DP;McComb, R;Ho, CC;Bradley, KA;Hartmann, S;Cheng, LW;Chugunova, M;Kao, CY;Tran, JK;Derbedrossian, A;Zilbermintz, L;Amali-Adekwu, E;Levitin, A;West, J;
Product: Anthrax Protective Antigen (PA), Recombinant from B. anthracis
Detection of PA and Anti-PA IgG in Serum
PA was measured in serum using PA-ECL screening assay kit MesoScale Discovery (L15CA-1), which utilizes the anti-PA antibody. To quantitate the levels of PA in each serum sample, a standard curve (0–100 ng/mL) was analyzed in parallel on each assay day by using recombinant PA (List Biological Laboratories, Inc., 171E). Test samples were assayed in duplicate.
• Product #171E – Anthrax Protective Antigen (PA), Recombinant from B. anthracis
June 14, 2021
IEEE Sensors Journal
Li, B;Wang, T;Bai, W;Su, Q;Wu, X;Dong, P;
Product: Anthrax Protective Antigen (PA), Recombinant from B. anthracis
MATERIALS AND METHODS
A. Proteins and reagents
Anthrax Protective Antigen (PA, recombinant from B. anthracis) was purchased from List Biological Laboratories Inc. (Campbell, California, USA). …
• Product #171E – Anthrax Protective Antigen (PA), Recombinant from B. anthracis
June 7, 2021
The Journal Of Experimental Medicine
Vijay, R;Guthmiller, JJ;Sturtz, AJ;Crooks, S;Johnson, JT;Li, L;Lan, LY;Pope, RL;Chen, Y;Rogers, KJ;Dutta, N;Toombs, JE;Wilson, ME;Wilson, PC;Maury, W;Brekken, RA;Butler, NS;
Product: Anthrax Protective Antigen (PA), Recombinant from B. anthracis
… anthrax protective antigen (recombinant from Bacillus anthracis; 0.5 µg/ml; List Biological Laboratories, Inc.), …
• Product #171E – Anthrax Protective Antigen (PA), Recombinant from B. anthracis
June 2, 2021
Microorganisms
Smith, K;Garman, L;Norris, K;Muther, J;Duke, A;Engler, RJM;Nelson, MR;Collins, LC;Spooner, C;Guthridge, C;James, JA;
Product: Anthrax Protective Antigen (PA), Recombinant from B. anthracis
2.3. LT Neutralization Activity (LTNA)
Recombinant PA and LF were purchased from List Biological (List Biological Laboratories Inc., Campbell, CA, USA). The ability of participant plasma to neutralize LT (LTNA) was adapted from the standard procedure from the CDC as previously described [29] using the J774.1 macrophage cell line (ATCC, Manassas, VA, USA). In brief, cells were plated overnight at 90,000 cells/well in a 96-well plate. Plasma samples were diluted 1:50 in culture medium, and then diluted for eight additional serial two-fold dilutions in dilution tubes, and then incubated with LT for 30 min at 37 °C (600 ng/mL of PA, 120 ng/mL of LF) before adding to cell cultures. Control wells included cells alone, cells with added PA only, LF only, or cells with PA and LF (LT). …
• Product #171E – Anthrax Protective Antigen (PA), Recombinant from B. anthracis
Author did not specify which Lethal Factor (LF) was utilized; List Labs provides the following LF:
• Prodcut #172 – Anthrax Lethal Factor (LF), Recombinant from B. anthracis
• Product #169L – Anthrax Lethal Factor (LF-A), Recombinant from B. anthracis Native Sequence
May 20, 2021
The Journal Of Physical Chemistry
Yamini, G;Kanchi, S;Kalu, N;Momben Abolfath, S;Leppla, SH;Ayappa, KG;Maiti, PK;Nestorovich, EM;
Product: Anthrax Protective Antigen (PA), Recombinant from B. anthracis
… PA63 was prepared from PA83 using furin79 or purchased from List Biological Laboratories, Inc. (Campbell, CA, USA) in the purified form. …
• Product #171E – Anthrax Protective Antigen (PA), Recombinant from B. anthracis
May 4, 2021
Cells
Ha, SD;Lewin, N;Li, SSC;Kim, SO;
Product: Anthrax Protective Antigen (PA), Recombinant from B. anthracis
Reagents—Protective antigen (PA) and lethal factor (LF) were purchased from the List Biological Laboratories (Campbell, CA, USA). …
• Product #171E – Anthrax Protective Antigen (PA), Recombinant from B. anthracis
Author did not specify which Lethal Factor (LF) was utilized; List Labs provides the following LF:
• Prodcut #172 – Anthrax Lethal Factor (LF), Recombinant from B. anthracis
• Product #169L – Anthrax Lethal Factor (LF-A), Recombinant from B. anthracis Native Sequence
March 9, 2021
Cell Reports
Phulphagar, K;Kühn, LI;Ebner, S;Frauenstein, A;Swietlik, JJ;Rieckmann, J;Meissner, F;
Product: Anthrax Lethal Factor (LF), Recombinant from B. anthracis
STAR★Methods
KEY RESOURCES TABLE
REAGENT or RESOURCE SOURCE IDENTIFIER
Chemicals, peptides, and recombinant proteins
Lethal factor List Biologicals Cat#172B
Protective antigen List Biologicals Cat#171E
Activation of BMDMs and human macrophages
All cells were grown, maintained and activated at 37°C and 5% CO2 in a water-jacketed incubator. 1 × 106 WT or Casp1/11 DKO murine macrophages were plated per 12-well in DMEM, 10% (v/v) FCS (DMEM complete medium) one day prior to stimulation. All BMDMs were primed with 200 ng/mL LPS from Salmonella Typhimurium for 2 h or left untreated and subsequently washed three times with serum- and phenol red-free DMEM supplemented with 2 mM L-glutamine (DMEM minimal medium). For the time course experiment (Dataset 1), cells were preincubated with 0 or 100 μM glyburide (#NBP2-30141, Novus Biologicals) for 15-30min before 2 mM ATP (#7699, Sigma) or 10 μM nigericin (N1495, Invitrogen) were added for 15, 30, 45, and 60 min. Lethal factor and protective antigen were added at 1ug/ml. …
• Product #171E – Anthrax Protective Antigen (PA), Recombinant from B. anthracis‘
• Prodcut #172 – Anthrax Lethal Factor (LF), Recombinant from B. anthracis
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