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July 31, 2024
European Journal Of Immunology
Szymanska, I;Bauernfried, S;Komar, T;Hornung, V;
Product: Anthrax Protective Antigen (PA), Recombinant from B. anthracis
April 10, 2024
Nature
Du, G;Healy, LB;David, L;Walker, C;El-Baba, TJ;Lutomski, CA;Goh, B;Gu, B;Pi, X;Devant, P;Fontana, P;Dong, Y;Ma, X;Miao, R;Balasubramanian, A;Puthenveetil, R;Banerjee, A;Luo, HR;Kagan, JC;Oh, SF;Robinson, CV;Lieberman, J;Wu, H;
Product: Anthrax Protective Antigen (PA), Recombinant from B. anthracis
March 4, 2024
Research Square
Mamedov, T;Gun, N;Gulec, B;Khozeini, H;Ungor, R;Yuksel, D;Arslan, A;Dilik, Z;Deniz, D;Goven, N;Mammadova, G;Hasanova, G;
Product: Anthrax Protective Antigen (PA), Recombinant from B. anthracis
Immunogenicity Studies of PA83 variants in Mice
Immunogenicity studies were performed with 3 and 10 μg of plant-produced dPA83(E), dPA83(P) and commercial recombinant PA (rPA, List Biological Laboratories, Campbell, CA, used as a positive control) proteins, administered intraperitoneally in seven- to eight-week-old Balb/c mice (6 animals/group). Mice were injected with three doses of protein samples adsorbed in 0.3% Alhydrogel at three-week intervals (day 0, 21 and 42). …
• Product #171E – Anthrax Protective Antigen (PA), Recombinant from B. anthracis
March 1, 2024
Cell Reports
Koller, B;Nguyen, M;Snouwaert, J;Gabel, C;Ting, J;
Product: Anthrax Protective Antigen (PA), Recombinant from B. anthracis
… Anthrax lethal toxin List Biological Laboratories Inc. #171B/#172B …
Treatments
See Table S1 for list of Figures/panels in each figure and corresponding dose and exposure time. General information for the methods is provided here. To induce cytokine release from macrophages, microglia and BMDM cells were primed for 4–5 h (or time indicated in Table S1)with 1 μg/mL of LPS in culture medium, and some cells were pre-treated with 1 μM of CP456773/MCC950 for 30 min before ATP stimulation. Cells were then pulsed with 5 mM ATP for 30 min. For cytokine induction by anthrax lethal toxin (LeTx), LPS-primed cells were treated with LeTx at a final concentration of 250 ng/mL (LF and PA) for 3 h. Cell supernatants were collected and frozen at −80°C until assayed.
• Product #171E – Anthrax Protective Antigen (PA), Recombinant from B. anthracis
• Prodcut #172 – Anthrax Lethal Factor (LF), Recombinant from B. anthracis
January 31, 2024
Microorganisms
Tessier, E;Cheutin, L;Garnier, A;Vigne, C;Tournier, JN;Rougeaux, C;
Product: Anti-Edema Factor from B. anthracis (Goat)
… goat anti-EF (773L, List Labs, Campbell, CA, USA), …
January 1, 2024
Thesis
Wohlfart, J;
Product: Anthrax Lethal Factor (LF), Recombinant from B. anthracis
2.4.4 Purification of PA and LF
PA83 was expressed and purified by the Barth group. After purification, PA83 was activated in vitro to PA63 and stored at -80 °C.
LF was purchased from List Biological Laboratories (see 2.1.7) and stored at -80 °C.
Author did not specify which Lethal Factor (LF) was utilized; List Labs provides the following LF:
• Prodcut #172 – Anthrax Lethal Factor (LF), Recombinant from B. anthracis
• Product #169L – Anthrax Lethal Factor (LF-A), Recombinant from B. anthracis Native Sequence
October 24, 2023
Journal Of Medicinal Chemistry
Harrison, D;Billinton, A;Bock, MG;Doedens, JR;Gabel, CA;Holloway, MK;Porter, RA;Reader, V;Scanlon, J;Schooley, K;Watt, AP;
Product: Anthrax Protective Antigen (PA), Recombinant from B. anthracis
… For the activation of NLRP3, nigericin was used at a final concentration of 5 µM. PA (List Biological Laboratories) and LFn-PrgJ (expressed and isolated at Nodthera) were added to yield final concentrations of 1 and 4 …
• Product #171E – Anthrax Protective Antigen (PA), Recombinant from B. anthracis
October 19, 2023
Protein & Cell
Gao, X;Teng, T;Liu, Y;Ai, T;Zhao, R;Fu, Y;Zhang, P;Han, J;Zhang, Y;
Product: Anthrax Protective Antigen (PA), Recombinant from B. anthracis
Animal challenge
Mice were randomly assigned to the control or experimental groups. Mouse TNF (CF09, Novoprotein, China)
and/or LT (LF (172C, List Biological Labs, USA) and PA (171E, List Biological Labs)) diluted in 200 μL of phosphate buffered saline (PBS) was intravenously injected into the mouse tail-vein with or without inhibitors of caspase (Z-VAD-FMK, 627610, Calbiochem, Germany) or p38α (TAK-715, HY-10456, MedChemExpress, USA) diluted at indicated doses. Mouse survival was monitored and recorded at indicated time points.
• Prodcut #172 – Anthrax Lethal Factor (LF), Recombinant from B. anthracis
• Product #171E – Anthrax Protective Antigen (PA), Recombinant from B. anthracis
October 5, 2023
Communications Biology
den Hartigh, AB;Loomis, WP;Anderson, MJ;Frølund, B;Fink, SL;
Product: Anthrax Protective Antigen (PA), Recombinant from B. anthracis
Inflammasome activation
Late-log cultures of Salmonella enterica serovar Typhimurium strain SL1344 (a kind gift from Dr. Brad T. Cookson, University of Washington) were grown in L-broth containing 0.3 M NaCl with shaking at 37 °C. Bacteria were washed, resuspended in PBS, and added at a multiplicity of infection of 10 bacteria per macrophage. For activation of NLRP1b, BMM from BALB/c mice were exposed to 1 µg/mL anthrax lethal toxin, prepared as a 1:1 mix of protective antigen and lethal factor (List Biological). …
• Product #171E – Anthrax Protective Antigen (PA), Recombinant from B. anthracis
Author did not specify which Lethal Factor (LF) was utilized; List Labs provides the following LF:
• Prodcut #172 – Anthrax Lethal Factor (LF), Recombinant from B. anthracis
• Product #169L – Anthrax Lethal Factor (LF-A), Recombinant from B. anthracis Native Sequence
October 4, 2023
Cell
Du, G;Healy, L;David, L;Walker, C;El-Baba, T;Lutomski, C;Goh, B;Pi, X;Fontana, P;Dong, Y;Ma, X;Miao, R;Devant, P;Puthenveetil, R;Banerjee, A;Kagan, J;Oh, S;Robinson, C;Lieberman, J;Wu, H;
Product: Anthrax Protective Antigen (PA), Recombinant from B. anthracis
… Anthrax Protective Antigen List Biological Laboratories Cat# 171E …
• Product #171E – Anthrax Protective Antigen (PA), Recombinant from B. anthracis