Bacterial Toxin Research Citations

Immuno-fluorescent Double Labeling

Biotinylated dextran amine anterograde tracing combined with cholera toxin B retrograde tracing:

Cholera toxin B (CTB) (Sigma Chemical, St. Louis, MO, USA) was used as the retrograde tracer (2% in PBS) to label pre-oculomotor neurons in the INC and was delivered into the III 5d after BDA injection. Sections (30 mm) were incubated in goat anti-CTB polyclonal antibody (List Biological Labs, Campbell, CA, USA) overnight at 4C. …

• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

IFN Enzyme-linked immunosorbent spot (ELISpot) assay

A 2-day, direct ex vivo, IFN- enzyme-linked immunosorbent spot (ELIspot) assay was
used to determine frequencies of peptide-specific T lymphocytes, a modification of that which we
have described previously (35).  Initially a fetal bovine serum (FBS)-based assay was used for
assessing immunity in the first 15 patients but then we switched to a human serum (HS)-based
assay because concerns that low signal-to-noise ratio (SNR) may interfere with reliable detection
of antigen-specific immune responses.  The SNR was 0.92 0.22 for the FBS-based assay and
1.74 0.19 for the HS-based assay (p=0.043).  On day 1, 2.5 105 PBMCs/well were plated in
triplicate into 96-well round bottom plates in 200 l of RPMI-1640 containing L-glutamine,
penicillin, streptomycin, and 10% AB (of FBS) serum (T cell medium) along with cyclin D1 (control)
peptide (MELLLVNKLKWNLAA)(35), FR30, FR56, FR76, FR113, FR238, FR protein (Sino
Biological Inc., Beijing, China), tetanus toxoid (TT) (List Biological Laboratories, Campbell, CA),
or Phytohemagglutinin (PHA) (Sigma-Aldrich, St. Louis, MO). …

• Product #191 – Tetanus Toxoid from Clostridium tetani

Induction of Experimental Autoimmune Encephalitis (EAE):

Mice were injected subcutaneously at two sites on the femoral region with 200 µl of a mixture of MOG ₃₅₅₅ peptide emulsified 1:1 with Freunds complete adjuvant (Difco Laboratories, Detroit, MI, USA). MOG treated mice were also boosted with pertussis toxin (List Laboratories, Campbell, CA, USA; 200 ng) I.P. on both the day of injection and also 48 h later. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… LPS ultra pure from Salmonella minnesota R595 List Biological Laboratories, INC. Cat# 434 …

• Product #434 – ULTRA PURE LPS from Salmonella minnesota R595 (Re)

EAE induction using myelin oligodendrocyte glycoprotein35–55 peptide

Reagents for EAE induction were prepared by dilution of myelin oligodendrocyte glycoprotein35–55 peptide (MOG; 2 mg/mL; AnaSpec, Fremont, CA) in UltraPure™ distilled water (Invitrogen, Life Technologies, Grand Island, NY) emulsified with equal parts of complete Freund’s adjuvant (BD Biosciences Franklin Lakes, NJ) containing 8 mg/mL Mycobacterium tuberculosis H37RA (#231131; BD Biosciences). MOG emulsion was transferred to 1 mL Luer-Lok syringes (BD Falcon) with 27G ½” needles. Pertussis toxin was diluted in UltraPure™ (2 ng/μL; List Biologicals Laboratories, Campbell, CA) and transferred to syringes as described above. While anesthetized under 5% isoflurane gas, 6- to 8-week-old female C57Bl/6 mice (Charles River Laboratories, Wilmington, MA) were induced with EAE by subcutaneous injection of 100 μL MOG emulsion near flanking regions of the tail (200 μL total per mouse). Mice were concomitantly administered 100 μL pertussis toxin via intraperitoneal (IP) injection. After 48 h, mice received a second IP injection of 100 μL pertussis toxin to complete the induction procedure. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Purified proteins … Prn, PT for ELISA assays were from List Biologicals. …

Author did not specify which List Labs Pertussis Toxin was utilized.  List Labs provides Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer and Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free).

• Product #187 – Pertactin from B. pertussis (69 kDa Protein)

EAE:

… Pertussis toxin (200 ng) (List Biological Laboratories, Campbell, CA) in PBS and 50 mM NaCl were administered ip on the day of immunization and again 48 h later. After induction, neurological deficits …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… The intraperitoneal treatment with diphtheria toxin (DT; List Biological Laboratories, Inc …

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

… A goat anti-cholera toxin, subunit B (CTB) antibody (List Biological Laboratories, Campbell, CA; #703, Lot 7032AA, …

• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

EAE:

… On days 0 and 2 post-immunization, 200 ng of pertussis toxin (PT) (List Biological, Campbell, CA) was administered intraperitoneally (ip) in 0.2 ml of PBS (Sigma, St. Louis,Minnesota). Mice were observed daily …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)