Bacterial Toxin Research Citations

Stimulation of monocytes with LPS:

… experiments were conducted with a predictable LPS-stimulatory effect. For this purpose, monocytes from six horses were incubated in 24 well plates with RPMI-1640 containing 10% DHS and E. coli 0111:B4 LPS (List Biologicals Inc., Campbell, CA) at either 1, 50 or 100 ng/ml. …

The effect of ePL on LPS-stimulated monocytes:

In the second series of experiments (summarized in Table 1) monocytes obtained from 6 horses were stimulated with 50 ng/ml E. coli LPS 0111:B4 and incubated with either RPMI-1640 containing 10% DHS or 10% ePL. Equine monocytes incubated in RPMI-1640 supplemented with 10% DHS or pooled ePL without LPS served as control groups.

To more accurately define the potential anti-inflammatory effect of ePL we included experimental groups containing an escalating concentration of DHS. Specifically, equine monocytes from the same six horses were incubated with RPMI-1640 containing 1, 5 or 10% of DHS and 0111:B4 LPS at 50 ng/ml plus 10% pooled ePL. …

• Product #201 – LPS from Escherichia coli O111:B4

… (St. Louis, MO). Smooth-type LPS from E. coli O55:B5 (List Biological Laboratories, CA) was used as an endotoxin. …

NOTE:  At the time this paper was written, Product #203 (5mg – LPS from Escherichia coli O55:B5) was utilized.

Product #203 (5 mg – LPS from Escherichia coli O55:B5) is no longer sold.

Product #203A (2.5 mg – LPS from Escherichia coli O55:B5) is available for purchase.

• Product #203A – LPS from Escherichia coli O55:B5

Drugs used were … CT (12.5 µg/ml, List Biologicals, Campbell, CA), ), …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

… Both EAE and CFA mice received an intraperitoneal injection of 300 ng of pertussis toxin (List Biological laboratories, Cedarlane, Canada) on the day of induction and 48 h later. Mice were monitored daily (34 days post EAE induction) 

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… Mice were given 200 uL rapamycin. ip at 0.4 mg/mL every 2 days during the treatment window, as described. Diphtheria toxin (DT) was purchased from List Biological Laboratories. (Campbell, CA; cat no. 150) and given to mice ip on days -8, -6, and -4 at a dose …

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

DTx Injection:

20 ng per gram body weight of DTx (List Biological Laboratories, USA) was injected intraperitoneally into Zbtb46-DTRWT chimeric mice 1 day prior to RV inoculation and DTx injections were then repeated every day for short-term experiments (mice were sacrificed at 7 d.p.i for T-cell assay) or every other day for long-term experiments (mice were sacrificed at 28 d.p.i. for ELISA assays). …

• Product #150 –  Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

Flow cytometry and cell sorting:

KI-hiPS cells were differentiated for 1012 weeks, dissociated with Accutase (Thermo Fisher Scientific) for 6090 min at 37 C, harvested through a 40 m cell strainer (BD Biosciences, San Diego, CA), and collected into keratinocyte culture medium (KCM; a 3:1 mix of DMEM without glutamine and Nutrient Mixture F-12 Ham; Thermo Fisher Scientific) supplemented with 5% fetal bovine serum (Japan Bio Serum, Hiroshima, Japan), 0.4 g/mL hydrocortisone succinate (Wako), 2 nM 3,3,5-triiodo-l-thyronine sodium salt (MP Biomedicals, Santa Ana, CA), 1 nM cholera toxin (List Biological Laboratory, Campbell, CA), …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

… The mice additionally received 200 ng Pertussis toxin (PTx, List Biological Laboratories, Inc.) intraperitoneally at the time of immunization and 48 h later. Intravital FLIM was performed on day 15 after immunization, ie at the peak of disease. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… Briefly, 10 ng STa-ovalbumin conjugates (provided by Dr. DC Robertson at Kansas State University) was used to coat each well of Costar plates (Corning Inc., Corning, NY) to titrate anti-STa antibodies, and 100 ng LT (List Biological Laboratories, Inc., Campbell, CA) was coated to each well of 2HB plates (Thermo Scientific, Rochester, NY) to titrate anti-LT antibodies. …

Lethal Toxin (LT) is PA + LF:

Author did not indicate which specific lethal factor was utilized.  List Labs provides Product #172 (Anthrax Lethal Factor (LF), Recombinant from B. anthracis) and Product #169 (Anthrax Lethal Factor (LF-A), Recombinant from B. anthracis Native Sequence).

• Product #171 – Anthrax Protective Antigen (PA), Recombinant from B. anthracis

… at 2 weeks after optic nerve injury by immunostaining with a GAP-43 antibody [1:2000; custom-made in sheep (Benowitz et al., 1988)] and at 6 weeks after optic nerve injury by immunostaining for the cholera toxin B fragment (CTB, 1% in 3 µl; List Biological), …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt