Bacterial Toxin Research Citations

… Pertussis Toxin (250 ng/mL; Incubation of human umbilical vein endothelial cells with 100 ng/mL impaired G i protein activity [Lee et al., 2011]; List Biological Laboratories 

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… In parallel to each immunization, 200ng of Bordetella pertussis toxin (List biological laboratories, inc., Campbell, CA, USA) was administrated by an intraperitoneal injection. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Tolerance induction, sensitization and challenge:

To study oral tolerance induction prior to whey sensitization, mice received daily, for a period of 6 days, an oral gavage with … Next, mice were sensitized by means of weekly oral gavage with 20 mg whey protein (in 0.5 ml PBS) supplemented with 10 µg cholera toxin (CT; List Biological Laboratories, Inc. California, USA) as an adjuvant. Sham-sensitized control mice received 10 µg CT in 0.5 ml PBS. …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

Immunohistochemistry:

… Primary antibodies … CTB (goat, 1:4,000, List Biological Laboratories …

• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

… CTB: Five adult (>100 day old) GAD-GFP mice were injected with cholera toxin subunit (CTB; List Biological Labs, Campbell, CA). Injections targeted the vibrissae representation …

<• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

Lethal toxin and signaling inhibitors:

Recombinant protective antigen and wild-type lethal factor or lethal factor mutant (E687C) were obtained from List Biologicals (Campbell, CA) …

In vitro lethal toxin assay with Rag1^-/- splenocytes or human tonsillar lymphocytes:

100,000500,000 splenocytes per well were incubated in a round bottom 96 well plate in IMDM media supplemented with 10% FBS, 100 U/ml pencillin, 100 U/ml streptomycin, IL-2 (20 ng/ml) and IL-7 (10 ng/ml). Human tonsillar lymphocytes (2×10⁶) were plated in 200 l RPMI media supplemented with 10% FBS, 100 U/ml penicillin, 100 U/ml streptomycin, non-essential amino acids, sodium pyruvate, IL-2 (20 ng/ml), IL-7 (10 ng/ml) and IL-1 (20 ng/ml). For initial lethal toxin experiments splenocytes/tonsillar lymphocytes were cultured in media containing in 0.1% serum to minimize the effect of serum on lethal factor enzyme activity. After the initial period of lethal toxin treatment (3 hrs), serum was replenished to a full concentration of 10%. Cells after toxin treatment were stimulated with recombinant mouse or human IL-23 (50 ng/ml) (eBioscience) for 18 hrs. Cell supernatants were harvested by centrifugation at 1,500 rpm for 5 minutes and then used for the measurement of IL-22.

In vitro lethal toxin assay with MNK-3 cells:

MNK-3 cells (an ILC3 cell line) were cultured in MNK-3 media (DMEM (High glucose), 10% FBS, 100 U/ml penicillin, 100 U/ml streptomycin, 2 mM Glutagro, 1 mM sodium pyruvate, 10 mM HEPES, 55 M -mercaptoethanol, 5 g/ml gentamicin, 10 ng/ml IL-7 and 10 ng/ml IL-15). For lethal toxin experiments MNK-3 cells were cultured in media containing 10 ng/ml IL-7. Cells were treated with lethal toxin for 2 hrs followed by IL-23 stimulation for 6 hrs. Cell lysates were analyzed for RNA and supernatants were analyzed for secreted IL-22 by ELISA.

Lethal Toxin (LT) = PA + LF

• Product #171E – Anthrax Protective Antigen (PA), Recombinant from B. anthracis

Author did not specify which Lethal Factor (LF) was utilized; List Labs provides the following LF:

• Prodcut #172 – Anthrax Lethal Factor (LF), Recombinant from B. anthracis
• Product #169L – Anthrax Lethal Factor (LF-A), Recombinant from B. anthracis Native Sequence

Reagents:

The following reagents and concentrations were used in experiments: 100 ng/ml Ultra-Pure LPS (List Biological Laboratories, Campbell, CA, USA), …

Author did not specify which List Labs LPS product was utilized in their research.  List Labs provides the following LPS products:  https://listlabs.com/product-information/lipopolysaccharides/

EAE:

… Pertussis toxin (List Biological Laboratories) was given iv at day 0 (200 ng) and at day 2 (400 ng) after immunization. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… After the left masseter muscle was exposed, a glass capillary pipet … filled with 0.5% cholera toxin B subunit (CTB, List Biological Laboratories, Campbell, CA, USA), was inserted into the muscle, and 0.1 µl of CTB was injected. … 

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

… The Pertussis toxin (PTX) was obtained from LIST Biologicals. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)