Bacterial Toxin Research Citations

… on the right and cholera toxin B subunit (CTB, choleragenoid, List Biological Laboratories, Campbell, USA) on the left. … After washing thoroughly in PBS, the sections were incubated in PBS containing a goat anti-CTB antibody (List Biological Laboratories …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt
• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

Materials:

… Escherichia coli O111 B4-derived LPS was obtained from List Biological Laboratories, Inc. (Campbell, CA, USA). …

Results:

… Monophenols had no effect on Nos2 mRNA expression. The effect of magnolol on Cox-2 and Nos2 mRNA expression was greater than that observed for the positive control treated with LPS.  Next, we investigated the effects of eugenol-related compounds on HO-1 mRNA expression. The results are shown in Figure 5A and B. bis-Eugenol up-regulated the expression of HO-1 mRNA at both 1000 M and 50 M, whereas the other compounds had no effect at either of the two concentrations. The up-regulatory effect on HO-1 mRNA expression was observed in the absence of LPS. The gene expression was compared to that of the control untreated with the other compounds. Also, we investigated the effects of curcumin and isoeugenol on LPS-stimulated Cox-2 mRNA expression in RAW264.7 cells. …

• Product #201 – LPS from Escherichia coli O111:B4

Mouse EAE model:

Female mice (8-10 weeks old) were immunized by a subcutaneous injection with 200 µg of myelin oligodendrocyte glycoprotein (MOG)3555 … Pertussis toxin (200ng; List Biological Laboratories; Campbell, CA, USA) was then injected intraperitoneally into the mice on days 0 and 2. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Induction of EAE:

EAE was induced with two sc injections of emulsion delivering 200 mg designated peptide and 0.4 mg M. tuberculosis in IFA per injection on days 0 and 7. Then 250 ng of pertussis toxin (List Biological) was concomi- tantly administered ip to mice on …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Toxin neutralization assay (TNA):

An in vitro toxin neutralization assay (TNA) was performed using a MTT [3-(4.5-dimethylthiazol-2-yl)2.5-diphenyltetrazolium bromide] in a colorimetric cell viability assay with the J774A.1 macrophage cell line as previously described by Hering et al. [18] with slight modifications by Ndumnego et al. [17]. Briefly, serial diluted goat serum was incubated with PA and LF (List Biological Laboratories, USA) at concentrations of 500 and 400 ng/mL respectively before addition to overnight incubated macrophage cells. …

Author did not indicate which specific lethal factor was utilized.  List Labs provides Product #172 (Anthrax Lethal Factor (LF), Recombinant from B. anthracis) and Product #169 (Anthrax Lethal Factor (LF-A), Recombinant from B. anthracis Native Sequence).

• Product #171 – Anthrax Protective Antigen (PA), Recombinant from B. anthracis

… ELISAs were performed as described previously (58). Briefly, 96-well plates were coated at 4C overnight with 1 g/well of recombinant PA or EF (List Biologicals, Campbell, CA). …

• Product #171 – Anthrax Protective Antigen (PA), Recombinant from B. anthracis 

Author did not specify which List Labs Anthrax Edema Factor was utilized.  List Labs provides Product #178A(Anthrax Edema Factor (EF), Recombinant from B. anthracis) and Product #173B (Anthrax Edema Factor (EF), Recombinant, Mutant S447N from B. anthracis).

EAE:

… The mice also received iv injec- tions of 200 ng of pertussis toxin (List Biological Laboratories, Campbell, CA) on days 0 and 2, and were administered ip 10 mg of anti-DR3 or control Ab (rat IgG) on day 0. Individual animals were scored daily for clinical signs of EAE …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… To induce acute liver injury, mice were intraperitoneally injected with LPS (10 g/kg; E. coli 055:B5; List Biological Laboratories, Campbell, CA, USA) …

NOTE:  At the time this paper was written, Product #203 (5mg – LPS from Escherichia coli O55:B5) was utilized.

Product #203 (5 mg – LPS from Escherichia coli O55:B5) is no longer sold.

Product #203A (2.5 mg – LPS from Escherichia coli O55:B5) is available for purchase.

• Product #203A – LPS from Escherichia coli O55:B5

…1 g/ml CRM 197 (List Biological Laboratories, Inc., Campbell, CA, United States) in carbonate buffer at 4 C for 24 h. The plates were washed twice in PBS containing 0.05% Tween 20 and blocked for 2 h with 200 l of 5% bovine serum albumin in …

• Product #149A – Diphtheria Toxin CRM₁₉₇, Mutant

Drug Treatments:

Nocodazole (EMD Chemicals, Inc., San Diego, CA) dissolved in DMSO (Calbiochem, San Diego, CA) is added at a final concentration of 15 M to disrupt microtubules42.  Latrunculin A (AdipoGen, San Diego, CA) dissolved in DMSO is added at a final concentration of 1.7 M to inhibit the polymerization of actin43.  Both drugs or the solvent DMSO are added to the buffered saline within the Petri dish at least 20 minutes prior to an experiment, and remain in the solution for the duration of an experiment, i.e., 40 min. C. difficile Toxin B (List Biological Laboratories, Inc., Campbell, CA) at a concentration 200 ng/ml was used to non-specifically inhibit Rho-GTPases. …

• Product #155 – Toxin B from Clostridium difficile