Bacterial Toxin Research Citations

Acute Lung Injury:

… BAL fluids were cleared of cells by centrifugation. All samples were stored at 80C until further analysis. Diphtheria toxin for depletion of CD11c⁺ cells in CD11c-DTR Tg mice was from List Biological Laboratories (Campbell, CA).

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

Experimental Procedures:

Animals – Cgrp-GFP^/ female mice (McCoy et al., 2012) (available from MMRRC:36773) were crossed with male Advillin-Cre^/ mice (Hasegawa et al., 2007) to generate double heterozygous CGRP^+/; Advillin-Cre^+/ (CGRP-DTR^+/) mice. Heterozygous offspring were used for all experiments and have one functional Calca allele. All mice were backcrossed to C57BL/6 mice for at least eight generations.

Diphtheria toxin (DTX; List Biological Laboratories, Product 150) was dissolved in sterile 0.9% saline and stored at 20C in aliquots that were thawed once. DTX from List Biological was used for all (of the following) experiments…(1) Sensory neuron markers in lumbar DRG from CGRP-DTR+/- mice. (2) CGRP-IR afferents ablated in hairy skin.  (3) Ablation of Mrgpra3-expressing (chloroquine receptor) DRG neurons in CGRP-DTR+/- mice. (4)  DTX-treatment does not affect cold or heat sensitivity in wild-type mice or body weight.

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

Animals:

To compare vocalizations emitted by hearing and deaf male mice, we used a mouse line that expresses DTRs in hair cells to induce deafness. CBA/CaJ mice had the human dtr gene inserted into the pou4f3 gene…We gave 49 P2 mice a systemic injection of DT (4 ng/g i.m.; List Biological Laboratories)…

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

ELISAs:

… We tested coating the plates with the following Ags, all diluted in PBS: nicked diphtheria toxin (DT; 10 g/ml; List Biological Laboratories), DTaP vaccine (1:333, Sanofi Pasteur), NP 36 CGG (10 g/ml), and NP 16 CGG (10 g/ml), along with a PBS-only plate. …

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

Trpm8^DTR mice and TRPM8 neuron ablation:

Two consecutive diphtheria toxin (DTx) intraperitoneal injections (50 g/kg in normal saline; List Biological Laboratories;…)were administered 3 d apart to both transgenic mice and their control littermates aged 8 weeks.

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

Depletion of TAMs in Braf-induced thyroid cancer mouse models:

Macrophage depletion in the bone marrow (BM), blood, spleen and peritoneal cavity was examined in Ccr2-DTR mice after treatment with diphtheria toxin (DT) 20 ng/g (List Biologicals, Campbell, CA ) intraperitoneally (i.p.) on alternating days for 7 days. Twenty-four hours following the last dose of DT, mice were euthanized by CO2 inhalation and tissue samples obtained for flow cytometry and/or immunohistochemistry (IHC) as described below.

To assess the effects of TAMs on PTC development, Tg-rtTA/tetO-Braf/Ccr2-DTR mice were fed dox-impregnated chow (2500 ppm; Harlan-Teklad) for 7 days with or without DT i.p. on alternating days beginning on day 0. On day 7 (24 hr after the last dose of DT), mice were euthanized by CO₂ inhalation and thyroids extracted for IHC. To examine the role of TAMs in established BRAF-induced thyroid cancers we treated Tg-Braf/Ccr2-DTR mice at approximately 6 and 12 weeks of age with or without DT on alternating days for 10 days. Mice were euthanized and thyroids extracted 24 hr after the last dose of DT for flow cytometry and IHC.

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

Animals and DT treatment:

To ablate MCH neurons, male mice between 8 and 12 weeks of age were injected twice with DT [50 ng/g, intramuscular (i.m.); 2 d apart; List Biologicals]. DT was dissolved in saline (SAL; 0.9% NaCl).

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

… DTx List Biological Lab., Inc., California, USA …

MHC-II-antigenspezifische molecules (50% CD11c-DTR 50% MHC-II-/–chimeras):

For this purpose, the various groups were treated as described above, starting one day before immunization until the end of the EAE, every 48 hours with DTX or PBS and examined for clinical signs of disease. …

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

In Vivo OT-I Adoptive Transfer:

After 6 days, CFSE dilution on OT-I CD8⁺ T cells was analyzed. For DC depletion, Itgax-DTR-EGFP bone marrow chimera mice were treated i.p. with 100 ng diphtheria toxin (List Biological Laboratories, Inc., Campbell, CA) 1 day before and after circular antigen transfer.

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

In vivo OT-I adoptive transfer:

For DC depletion, Itgax-DTR-EGFP bone marrow chimera mice were treated i.p. with 100ng diphtheria toxin (List Biological Laboratories, Inc., Campbell, CA) 1 day before and after circular antigen transfer.

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae