Bacterial Toxin Research Citations

Preparation of Mice:

…Diphtheria toxin (List Biologicals, Campbell, CA) was administered intraperitoneally as described in the online supplement. …

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

Mice:

For Cre activation, tamoxifen…was given via gavage to neonatal mice (0.075mgg¹ for Lgr5EGFP-CreERT2 and 0.75mgg¹ Plp1-CreERT strains) and dams (0.225mgg¹, i.p.). Diphtheria toxin (6.25ngg¹ i.p., List Biological Laboratories)…

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

… Purified CT (Cat. No. 100B) and diphtheria toxin (Cat. No. 150) were purchased from List Biological Laboratories (Campbell, CA, USA). …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae
• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

DC Depletion with Diphtheria Toxin:

Mgl2-DTR and huLangerin-DTR mice were i.p. injected with 1 mg of diphtheria toxin (List Biological Laboratories) 2 days before infection,…

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

Diphtheria toxin-induced monocyte depletion mouse model:

Itgam(CD11b)-DTR mice were obtained from Jackson Laboratories. CD11b-DTR and C57BL/6 WT mice were injected i.p. with 100 l of diphtheria toxin (List Biological Laboratories, Inc. Campbell, CA) at a concentration of 0.02 g per gram of mouse weight. Two days after PBS or CpGB DNA pump insertion, skin was collected for RNA isolation or immunofluorescence.

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

Reagents and antibodies:

… Un-nicked Diphtheria Toxin was purchased from List Biological Laboratories, Inc. (Campbell, CA) and was used to deplete Foxp3⁺ Tregs from DEREG mice [49] by administering 1g i.p. DT per two days from day -2 to day six p.i. …

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

CCR2⁺ cell depletion strategy:

For depletions of CCR2⁺ cells, CCR2-DTR mice and control littermates received 250 ng of diphtheria toxin i.p. one day prior to infection. Diphtheria toxin was purchased from List Biological Laboratories (Campbell, CA) and reconstituted at 1 mg/ml in PBS. Aliquots were stored at 80C. The specificity and efficiency of depletion in the lung was confirmed by flow cytometry.

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

… Diphtheria toxin (DT) was administered to DTR mice genetically engineered to express the human diphtheria toxin receptor selectively in hair cells (Tong et al., 2011; Golub et al, 2012). A 50g/Kg dose of DT (List Biological Laboratories, Inc. #150) was delivered via intramuscular injection to four week old DTR mice. …

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

Animal care and use:

…Diphtheria toxin (DTX, List Biologicals) was injected as described previously [3], and does not affect sensory responses or body temperature when administered to wild-type mice, as previously described [3].

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

Cell Deletion:

Deletion of Jun and JunB was verified by PCR. Similarly, 300 ng of Diphtheria toxin (DT, List Biological Laboratories, in PBS) was injected intraperitoneally into experimental mice according to the schemes indicated in the figures. For LC depletion, DT was applied every third day, and for pDC depletion, every other day.

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae