Bacterial Toxin Research Citations

Purified diphtheria toxin was obtained from List Biological Laboratories Inc. (Campbell, CA).

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

… To deplete dendritic cells, CD11c-DTR mice were treated i.v. with 100ng diphtheria toxin in PBS (List Biological Laboratories) one day prior to SMARTA cell transfer. A second treatment was given one day after cell transfer. PBS vehicle was injected into undepleted controls.

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

Mice:

Tumors that were 69 mm in diameter were selected, and volumes were monitored by high-resolution ultrasound as detailed in… KPC (DTR transgene) mice were treated every 48 h with 25 ng/g of DTx (List Biologicals) in PBS,…

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

Adult ablation of MCH neurons improves glucose tolerance, even in absence of MCH signaling:

MCH neurons increase their firing rate in situ when extracellular glucose increases (Burdakov et
al., 2005). Ten days after DT injection, MCH-neuron-ablated mice showed reduced area-under
the-curve (AUC) during a glucose tolerance test … Hypothesizing that this phenotype of MCH-neuron
ablation resulted from the loss of a non-MCH neurotransmitter in MCH neurons, I measured
glucose tolerance in homozygous PmchDTR/DTR mice injected with DT.

… Some vendors have proven more reliable than others at producing DT that is free of side effects in wild-type mice. We have had good luck with DT from List Biological Laboratories, but problems with that obtained from Sigma-Aldrich. DT comes lyophilized in vials of 1 mg. …

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

Diphtheria toxin-induced monocyte depletion mouse model:

… CDllb-DTR and C57Bl/ 6 WT mice were injected i.p. with 5001..11 of diphtheria toxin (List Biological
Laboratories, Inc.) at a concentration of 21..1g/ ml the day before pump insertion. …

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

Pharmacological injections: 

Pharmacological compounds were prepared in sterile 0.9% saline and stored at 20 C prior to use. Diptheria toxin (DT) (50 g/kg; List Biologicals, #150), was injected intramuscularly (in mice) and all other compounds were administered intraperitoneally.

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

Immunization:

All immunization procedures were conducted in the rear footpad with 20 l injection volume per footpad. In experiments involving DT injection, 0.5 g DT (List Biological Laboratories) was injected intraperitoneally. For in vivo antigen-uptake experiments, WT B6 mice were injected in both hind legs with 2 g Alexa Fluor 488-conjugated OVA together with 50 g papain (Sigma) or CpG2216 (Invivogen).

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

Pharmacological Treatments:

To ablate AgRP neurons in adult mice, we administered two i.m. injections of DT (50 mg/kg; List Biological Laboratories) 2 d apart to 6-wk-old mice…

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

Mice and in vivo treatments:

DC depletion (in mice reconstituted with CD11c-DTR marrow) was accomplished by injection of diphtheria toxin (DT) (5 ng/g, IV; List Biological) 2 days in a row.

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

Materials and Methods:

…To ablate macrophages in Itgam-Dtr transgenic mice or lysozyme M Cre Dtr fl/fl mice, 20 ng/g body weight diphtheria toxin (List Biological Laboratories, Campbell, CA) was injected intraperitoneally at 1 day before laser induction and 1 day after laser injury. …

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae