Bacterial Toxin Research Citations

DT treatment and blood sampling of mice:

GHCre/iDTR mice and Cre-negative control littermates (referred to as /iDTR) of the indicated ages were injected ip with DT (4-ng/g body weight, twice a day at 9 am and 5 pm; List Biological Laboratories) for either 3 (3-d DT treatment [3DT]) or 10 (10DT) consecutive days. Three to 6 mice were used per condition in the independent experiments. …

Blood samples (100 L) were taken retroorbitally at 9 am, first on the day before the start of DT treatment and then at 11 days (d11), 1.5, 12, and 15 months after DT initiation. …

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

Experimental autoimmune encephalomyelitis:

For DC depletion, diphtheria toxin (12 ng/g bodyweight; List laboratories, UK) was diluted in PBS and administered intraperitoneally, starting the day prior to immunization and repeated every 48 h until the end of the experiment. Depletion efficiency was checked on spleen and CNS samples…

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

We temporarily depleted CD207⁺ DCs by injecting diphtheria toxin (DT) into MuLanDTR mice that express transgenic receptors for DT (DTR) in all CD207⁺ DCs³⁵. Compared to untreated MuLanDTR mice, MuLanDTR mice treated with DT for 8 days had reduced percentages of CCR10⁺MHCII⁺ ILCs in sLNs but not in the skin

Diphtheria toxin treatment:

Mice were injected intraperitoneally with 1g diphtheria toxin (List Biological Labs, Inc, Campbell, CA) per week and analyzed 821 days after injection.

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

Diphtheria Toxin Treatment:

Mice were treated with 0.2 g/mouse diphtheria toxin (List Biologicals) by i. p. injection every – other – day unless otherwise described in figure legends . 

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

Compound preparation and delivery – Diphtheria toxin:

Diphtheria toxin (DT) (Product #150, List Biological Laboratories, Inc., Campbell, CA) was
administered in a single, 300uL i.p. injection of 0.5, 1.0, 2.5, 5.0, 10, or 25 ng total DT. Control
NSG-DTR mice (PBS) were treated with an equal volume of 1X PBS (Sigma, St. Louis, MO).
All animals in a cohort (with human islets from the same human donor) were injected with DT or
PBS on the same day. …

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

Immunizations and Infections:

For Treg ablation experiments, Foxp3-DEREG mice were injected intraperitoneally with 75 g/kg diphtheria toxin (List Biologicals) at days 1 and 2 post antigen immunization.

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

Characterisation of the model:

Podo-DTR mice and wild-type littermates as controls were injected with a single dose of diphtheria toxin (DT) (Product no. 150, Lot 15023A1; List Biological Laboratories Inc., California, USA) ranging from 0.1166ng/g body weight (bw). …

ACEi protection experiment:

Groups of 16 transgenic (Tg) mice and eight wild-type (WT) littermates were given captopril (200mg/L) (Product no. C4042, Sigma Aldrich, UK) in their drinking water or placebo (water alone) 24h after a single intraperitoneal (i.p.) injection of diphtheria toxin at 5ng/g bw (Product no. 150, Lot 15023A1; List Biological Laboratories Inc., California, USA). …

Urine and blood analysis:

Urine and serum creatinine concentrations were measured using the creatinase reaction, with the exception of the first cohort of the model evaluation studies where Jaffe reaction was used (for the Podo-DTR line 47 given 1ng/g DT).

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

Un-nicked Diphtheria Toxin was purchased from List Biological Laboratories, Inc. (Campbell, CA) and was used to deplete Foxp3+ Tregs from DEREG mice (Lahl et al., 2007) by administering 1g i.p. DT per 2 days from day -2 to day 6 p.i. …

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

Dendritic Cell and T- and B-Cell Depletion:

CD11c-DTR-BM chimeric heart graft recipients were treated with i.p. 32 ng/g diphtheria toxin (List Biological Laboratories) on days 3, 1, and +1 in relation to transplantation and thrice weekly thereafter.

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

Drugs and Peptides:

DT (50 g/kg, List Biological Laboratories) or vehicle injection was administered via subcutaneous or intramuscular routes in neonates or adult animals (mice), respectively.

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae