Bacterial Toxin Research Citations

Polyclonal CD4+ T cell adoptive transfer:

…In some cases, Foxp3-expressing Treg cells were ablated following adoptive transfer by the i.p. injection of diphtheria toxin (DT; List Biological, Campbell, CA) at a concentration of 0.5 g/mouse on day 1 after adoptive transfer of T cells, and then 0.2 g/mouse every other until day 21. …

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

Mice and Reagents:

… and Diphtheria toxin ( DT ) from Corynebacterium diphtheriae (no. 150) were purchased from List Biological Laboratories Inc. …

TLR stimulation induces a second wave of intimal CD11c+ cell reduction, not mediated by apoptosis, but is dependent on CCR7 and CCL19 expression:

(a) … Positive controls were Cd11c-dtr transgenic mice injected with diphtheria toxin (DT) for 8h.

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

DT preparation and administration:

DT (List Biological Laboratories Inc., catalog 150) was administered to NSG-DTR mice in a single, 300 l i.p. injection of 0.5, 1.0, 2.5, 5.0, 10, or 25 ng total DT. Control NSG-DTR mice (PBS) were treated with an equal volume…

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

DT and clodronate liposome injections:

DT (lot number 15043A1, diluted in PBS; List Biological Laboratories) was administered via i.p. injection on day 22 (50 mg/kg mouse) and day 21 (15 mg/kg) of the priming period prior to i.t. LPS as described previously (39). Mice harvested on day +5 also received a DT dose (15 mg/kg) on day +2 after i.t. LPS. Clodronate liposomes (Cl2MDP) or PBS liposomes (control) were prepared as previously described (8), followed by o.p. instillation (60 ml) on day 23, and i.p. injection (500 ml) on days 22 and 21 of the priming period.

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

Diphtheria toxin and clodronate liposome injections:

Diphtheria toxin (lot no. 15043A1; List Biological Laboratories) was diluted in PBS and administered via intraperitoneal injection on days 2 (50 g/kg mouse) and 1 (10 g/kg) before intratracheal LPS, and on days +1, +3, and +5 (10 g/kg) following intratracheal LPS as previously described (52). …

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

Cell Depletion:

KPCD mice received one intraperitoneal injection of DT (25ng/g body weight; List Biological Laboratories) or matched volume of PBS. Monocyte depletion was analysed 24h later by flow cytometry.

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

Polyclonal CD4⁺ T cell adoptive transfer (in mice):

Foxp3-expressing T_reg cells were ablated following adoptive transfer by the i.p. injection of diphtheria toxin (DT; List Biological, Campbell, CA) at a concentration of 0.5 g/mouse on day 1 after adoptive transfer of T cells, and then 0.2 g/mouse every other until day 21.

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

In Vivo Depletion and Transfer of Donor and Recipient Leukocyte Subsets:

Hematopoietic cells were depleted as previously described…by lethal irradiation (2  6.5 Gy) of donor mice 7 days before heart allograft procurement. B6.CD11c-DTR, BALB/c.CD11c-DTR, and CB6F1.CD11c-DTR donors and C57BL/6 (BL/6.CD11cDTR) chimeric heart graft recipients were treated with i.p. 32 ng/g DT (List Biological Laboratories) on days 5, 3, and 1 (donors) or thrice weekly in recipients.

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

DT Depletion of CD11b⁺ Cells:

Newborn mice within 12 hours of birth were injected intraperitoneally with 500 ng DT (List Biological Laboratories, Campbell, CA) diluted in sterile PBS (50 l total). Injected mice were returned to their cage and placed in hyperoxia or normoxia conditions.

• List Labs Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

In vivo treatments:

To deplete pDCs, BDCA2-DTR mice were injected i.p. with 20 ng/g of DT (List Biological Laboratories, Campbell, CA, USA) on day 0 (or day 1 for oral immunization), then every other day with 10 ng/g. DT-treated WT littermates were used as controls. …CD4⁺ T-cell depletion was controlled in blood at day 0 before starting DT treatment and at sacrifice.

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae