Bacterial Toxin Research Citations

Chemicals and Reagents:

Diphtheria toxin (DT) was obtained from List Biological Laboratories (Campbell, CA. Cat. No. 150), reconstituted in PBS and stored at 80C. Mice received a dose of DT (10ngg¹ in 0.1ml PBS)³⁰, injected intraperitoneal, every other day.

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

… Targeted elimination of CX3CR1+ cells. CX3CR1-DTR mice were treated with diphtheria toxin (DT, List Biological Lab Inc., Campbell, CA) three times over seven days using a dose of 200ng/20g mouse. …

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

Drugs:

The following compounds were used in the reported studies: CL 316,241 (in saline; from Tocris); C75 (RPMI medium 1640; from Tocris);67,68 capsaicin (3.3% Tween-80 in saline; from Sigma)14, CNO (in saline; from Enzo Life Science)14, and diphtheria toxin (in saline; List Biological, cat. no. 150). All drugs were injected in a volume of 10 ml kg1 of body weight i.p. except for diphtheria toxin, which was injected at the dose of 50 g kg1 and the volume was 2.66 l g1 of body weight. When multiple injections were performed in the same experiment, the volume of each injection was adjusted to a total volume of 10 ml kg1 per animal.

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

… For experiments with TLR7.1/Ccr2- DTR mice, mice were injected intraperitoneally with 10 ng DT/g of body weight (List Biological Laboratories) in phosphate-buffered saline every other day for 17 days.

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

… All mice used were between 8 and 12 weeks old. Diphtheria toxin (DT) (List Biological Laboratories, Inc., Campbell, CA) was resuspended in 1X PBS and mice were injected ip with 150 ng/g DT in 100 L of PBS every 24 hours (hrs) for 3 days …

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

… Diphtheria toxin (DT; 50 g/kg body wt; List Laboratories, Campbell, CA) was administered once (in the absence of UUO) or at stated times after UUO via ip doses …

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

… Cancer Immunology, Immunotherapy 1 3 administered ip in 200 L of 0.4 mg/mL solution; mice were treated every 2 days beginning day 9, as described [22]. Diphtheria toxin (DT); (List Biological Laboratories, Campbell, CA; cat no 150 …

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

… For FAP+ cell deletion, Diphtheria Toxin (DTx) (List Biological Laboratories) was administered by 390 i.p. injection to 8 week old FAP-DTR mice at a dose of 25ng/g, twice a day, in a prophylactic regime (at days -7 and -5 post STIA) and in a therapeutic regime (at days 3 and 5 or at days 5 and 7 post STIA or at days 10 and 12 393 post STIA). …

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

Reagents and antibodies:

… DT was purchased from List Biological Laboratories, …

FRC depletion using DT:

CCL19^Cre  iDTR mice were injected with 100 ng of DT i.p. on day 2, day 1, and day 0 prior to IRI.

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

Diphtheria toxin List Biological Labs Part# 150

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae