Bacterial Toxin Research Citations

Cell Ablation:

For ablation of neurons expressing Cre and FlpO drivers in addition to Tau^ds-DTR, mice were injected with diphtheria toxin (DT; 50 ng kg^-1 in 0.9% sterile saline, i.p.; List Biological Laboratories) at P28 and P31 (Bourane et al., 2015a). Analysis of spontaneous scratching in mice expressing NPY::Cre (and relevant controls) was performed at P35, 7 days following the first DT injection. For the double-Cre ablation experiment, controls were positive for Cre and Tau^ds-DTR alleles but lacked the Lbx1^FlpO allele and did not therefore express the diphtheria toxin receptor; however, all mice received DT. …

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

Immunohistochemistry:

The following primary antibodies were used in this study: …  goat -CTB (1:4000; List Laboratories), …

• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

… Diphtheria toxin List Biological Laboratories, Inc Un-nicked, Lot #: 15043A1 …

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

… In vivo depletion of Foxp3 + Treg Foxp3 + Treg were depleted from DEREG mice[10] by injection of 500ng Diphtheria toxin (DT) (List Biological Laboratories, Inc., Campbell, CA) and two days later mice were grafted as described below …

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

Materials:

…  DT and CT were purchased from List Biological Laboratories (Campbell, CA). …

Intoxication assays:

HeLa or CHO cells grown to ~80% confluency in 24-well plates were challenged with varying concentrations of DT or CT in serum-free medium. Where indicated, cells were also exposed to 20 M PES for 1-2 h or 10 M CysA for 30 min before toxin addition. …

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

Author did not specify which Cholera toxin was utilized.  List Labs Product #101B/C – Cholera Toxin from Vibrio Cholerae has been discontinued; however, List Labs still provides Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae.  

To follow, are our recommendations for transitioning from Product #101B/C to Product #100B:

Product #101B, Cholera Toxin from Vibrio cholerae (1 mg), has been discontinued.  Product #100B, Cholera Toxin (AZIDE-FREE) from Vibrio cholerae, may be substituted for Product #101B, if the user accounts for the formulation differences.  Both products contain the same quantity of toxin per vial (1.0 mg).  For Product #100B, reconstitution with 1.0 mL of water results in a 1 mg/mL toxin solution in a buffer with the following component concentrations:  0.05 M Tris, 0.2 M NaCl, 0.001 M Na2EDTA at pH 7.5.  For Product #101B, reconstitution with half that amount of water (0.5 mL) resulted in a 2 mg/mL toxin solution with the same concentrations of the buffer components, and in addition, Product #101B contained 0.003 M NaN3.  The user may add the sodium azide preservative to product #100B, if desired.

Product #101C, Cholera Toxin from Vibrio cholerae (2 mg), has been discontinued.  Product #100B, Cholera Toxin (AZIDE-FREE) from Vibrio cholerae, may be substituted for #101C, if the researcher accounts for the formulation differences.  While Product #101C contained 2.0 mg per vial, Product #100 contains 1.0 mg per vial.  When Product #100B is reconstituted with 1.0 mL of water, the resulting 1 mg/mL toxin solution will have buffer components with the following concentrations:  0.05 M Tris, 0.2 M NaCl, 0.001 M Na2EDTA at pH 7.5.  For Product #101C, reconstitution with less than half that amount of water (0.4 mL) resulted in a 5 mg/mL toxin solution in the same buffer, and in addition, Product #101C contained 0.003 M NaN3.  The user may add the sodium azide preservative to product #100B, if desired. 

Large quantities of Product #101B or Product #101C may be obtained through Bulk Requests and Customer Orders. 

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

… Diphtheria toxin (List Biological Laboratories) was administered by intraperitoneal injection of FAP-DTR mice (25 ng/g), twice a day, both in prophylactic (at day 7 and 5 before STIA) and in therapeutic regimes (at days 3 and 5 or at days 5 and 7 or at days 10 and 12 after STIA). …

• Product #150 – Diphtheria Toxin, Unnicked, fromCorynebacterium diphtheriae

… Diphtheria Toxin (unnicked) List Biological Laboratories Cat # 150 …

• Product #150 – Diphtheria Toxin, Unnicked, fromCorynebacterium diphtheriae

Mice:

C57BL/6J (stock #000664), MHCII^fl/fl (stock #013181) [89], and Arg1^fl/fl (stock #008817) [90] mice were purchased from the Jackson Laboratory (JAX). CCR2^-/- mice (JAX stock #004999) [34] were generously provided by Dr. E Pamer (MSKCC). Rosa26^flSTOP-tdRFP mice [36] were generously provided by Dr. J. Sun (MSKCC) [91]. The dblGATA mice [50] on a C57BL/6 background were generously provided by Dr. H. Rosenberg (NIH). Vav1-Cre mice (JAX stock #008610) [92] were generously provided by Dr. F Geissmann (MSKCC). RAG2^-/-c^-/- mice (stock #4111) [93, 94] were purchased from Taconic. CD45.1⁺ mice (stock #564) were purchased from Charles River Laboratories. The CCR2-DTR depleter mice and CCR2-GFP reporter mice were generated as previously described [5, 95]. All mouse strains were bred and housed in the Memorial Sloan Kettering Cancer Centers (MSKCC) Research Animal Resource Center under specific pathogen-free conditions. Mice on the RAG^-/-c^-/- background were maintained on amoxicillin- and Vitamin E-containing chow. To ablate monocytes, CCR2-DTR or CCR2-DTR RAG^-/-c^-/- mice and their control littermates were injected intraperitoneally with 200 ng (10 ng/g body weight) of diphtheria toxin (List Biological Laboratories) every other day for three doses, starting the day before infection (Fig 1B), unless otherwise noted. All experiments were conducted using male and female mice at age 68 weeks with sex- and age-matched mice in experimental and control groups. Experiments with CCR2-DTR, CCR2-DTR RAG^-/-c^-/-, CCR2-Cre and Vav1-Cre mice used littermate control mice that were weaned from the same litters and co-housed.

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

Mice:

For selective macrophage depletion, CD11b-DTR mice were injected intravenously (i.v.) with 30ng/g body weight of DT (List Biological Labs) at 36h post APAP challenge.

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

… To induce HC death in Atoh1-DTA mice, tamoxifen (Sigma-Aldrich) was dissolved in 100% corn oil and injected IP at 3 mg/40 g on P0 and P1. For fate-mapping experiments, tamoxifen (3 mg/40 g) was injected IP at P0 only to induce tdTomato expression in SC subgroups, followed by injection of DT (6.25 ng/g, IM; List Biological Laboratories, Inc.) at P1 to induce HC death in Pou4f3DTR mice …

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

DT administration:

DT (List Biologicals Laboratories) was suspended in PBS. Stock solutions were thawed once, and mice were injected i.p. with DT as previously described …

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae