Bacterial Toxin Research Citations

Mouse breeding and treatments:

…DT (List Biological Laboratories Inc.) was injected i.p. at 10 ng/g of body weight every 48 hours for a total of 4 injections. …

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

Subjects and experimental outline:

In total, 40 mice (both genders) were used in this experiment with 20 each assigned to the control (Cre) and experimental (Cre⁺) groups. All subjects in both groups received one intraperitoneal injection (i.p.) of DT at a dose of 25 ng/g (List Biological Laboratories, Campbell, CA, USA) at postnatal day 21. …

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

…Diphteria toxin (DT) was obtained from List Biological Laboratories (Campbell, CA), …

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

Mice, BM chimeras and in vivo treatments:

…For experiments with Ccr2-depleter mice, mice were injected ip with 10 ng/g Diphtheria Toxin (DT) (List Biological Laboratories) in PBS. …

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

For the ablation experiments, bigenic Gli1CreERt2; iDTR mice received tamoxifen (3x 10mg per oral gavage) 10 days prior to irradiation and BM transplantation and were injected with diphtheria toxin dissolved in PBS (List Biological Laboratories) at a dose of 50ng/g body weight intraperitoneally (i.p.) as indicated (Figure 3B).

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

For megakaryocyte depletion, mice received daily i.p. injections of 250ng diphtheria toxin (List Biological Laboratories, 150) or PBS control. Megakaryocytes depletion was confirmed by complete blood count analyses and immunofluorescence staining.

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

For experiments with Ccr2-depleter mice, mice were injected intraperitoneally with 10 ng/g diphtheria toxin (DT) (List Biological Laboratories) in phosphate-buffered saline.

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

Diphtheria toxin administration:

Diphtheria toxin (DT) (List Biologicals Laboratories, Campbell, CA) was suspended in PBS. Stock solutions were thawed once, and mice were injected intraperitoneally with Diphtheria toxin as previously described (1, 3). …

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

Drug treatments:

…For fate-mapping studies in damaged utricles, tamoxifen (6 mg/40 g body weight, IP) was injected once on two consecutive days in 9-week-old Plp1-CreER^T2:ROSA26^tdTomato:Pou4f3^DTR mice, followed one week later by two intramuscular (IM) injections of DT. Each injection was 25 ng/g body weight, and there was a 48 hr interval between injections. DT was purchased from…List Biological Laboratories, Inc. (Campbell, CA). The different tamoxifen doses produced similar labeling of utricular SCs. …

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

Diphtheria toxin treatment:

Diphtheria toxin (DT) was administered to DTR mice, genetically engineered to express the human DTR selectively in hair cells (Tong et al. 2015). A single 25 g/kg dose of DT (List Biological Laboratories, no. 150) was delivered via intramuscular injection to 4-wk-old DTR mice. Within 6 days after DT injection, DTR mice lose all of their hair cells and are completely deaf (Tong et al. 2015). After DT injection, DTR mice were allowed to survive for 2 wk before tissue collection.

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae