Bacterial Toxin Research Citations

… Mice were anesthetized by isoflurane and dorsal fur was removed with a clipper. A sub-cutaneous injection of 106 PDA cells was performed. Tumors were grown for 2 weeks. In order to eliminate the tumors, diphtheria toxin (DTx, List Biologicals, #150) was injected intraperitoneally on two consecutive days at a dose of 25ng/g. …

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

In vitro diphtheria toxin protection assay:

Mouse serum samples were first diluted 1:10 in RPMI containing 10% FBS and then serially diluted in 384-well plates using Bio-Tek Precision 2000 liquid-handlers (Bio-Tek, Winooski, VT). Forty L of 100 ng/mL Diphtheria toxin (List Biological Laboratories, Campbell, CA) was added to each sample well and incubated for 1h at 37C. …

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

… Diphtheria toxin (List labs) at 10 ng/g body weight was administered intranasally at P18 and P22. …

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

… The intraperitoneal treatment with diphtheria toxin (DT; List Biological Laboratories, Inc …

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

… Diphtheria toxin (30 g/g of mouse; List Biological Laboratories, INC) was 285 injected subcutaneously either at postnatal day (P) 1 or P5 and the brains were 286 collected at various ages (Fig. 1a and Fig. 5a) 

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

Discussion:

The proportionality model of intoxication suggests the cytosolic stability of the toxin A chain will directly impact the capacity for recovery from intoxication, which is consistent with our observations for Dtx: as shown in Figs 56, cells could only recover from a minimal initial inhibition of protein synthesis by this relatively stable toxin35. Other reports further suggest the extent of intoxication is linked to the efficiency of toxin clearance from the cytosol. For example, cellular resistance to ricin or EtxA results from enhanced degradation of the cytosolic toxin33,34,36. Conditions that impede the turnover of cytosolic toxin likewise generate cellular sensitivity to ricin or Stx15,32,33,36. These collective observations indicate the extent of intoxication is directly linked to how much toxin is in the cytosol, with the amount of cytosolic toxin representing a balance between toxin delivery to the cytosol and toxin removal from the cytosol.
 

Toxins:

… EtxA and Dtx were purchased from List Biologicals (Campbell, CA). …

• Product #160 – Exotoxin A from Pseudomonas aeruginosa
• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

Diphtheria Toxin List Biological Labs 150

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

For T reg cell depletion by DT, Foxp3EGFPDTR+ mice were treated i.p. with 50 g/kg DT (List Biological Laboratory).

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

… Mice were given 200 uL rapamycin. ip at 0.4 mg/mL every 2 days during the treatment window, as described. Diphtheria toxin (DT) was purchased from List Biological Laboratories. (Campbell, CA; cat no. 150) and given to mice ip on days -8, -6, and -4 at a dose …

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

DTx Injection:

20 ng per gram body weight of DTx (List Biological Laboratories, USA) was injected intraperitoneally into Zbtb46-DTRWT chimeric mice 1 day prior to RV inoculation and DTx injections were then repeated every day for short-term experiments (mice were sacrificed at 7 d.p.i for T-cell assay) or every other day for long-term experiments (mice were sacrificed at 28 d.p.i. for ELISA assays). …

• Product #150 –  Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae