Bacterial Toxin Research Citations

… For ablation of cDC1 studies, WT and XCR1DTR were injected i.p., with 25 ng diphtheria toxin (DT) (150, List Biological Lab) per gram of body weight on day 6 post tumour engraftment …

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

Materials and Methods

1.1 “Main reagents and instruments” Diphtheria toxin (diphtheria toxin, DT, List Biological Laboratories, USA); …

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

… To deplete Tregs, diphtheria toxin (DT) (List Biologicals Laboratories, Campbell, CA) was given intraperitoneally to Foxp3DTR or Foxp3EGFP mice before and after LPS …

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

Treg cell isolation and adoptive transfer

… Diphtheria toxin (List Biologicals, CA) was administered via intraperitoneal injection in 100µL of sterile PBS in the following doses and days relative to influenza A virus infection (day 0): 50 mcg/Kg on day -2 and 10 mcg/Kg every 48 hours starting on day 0 and ending on day 28 post-infection. Five days later, 1 x 106 young or aged splenic Treg cells in 100 µL of sterile PBS were transferred via retro-orbital injection into the
influenza-treated Foxp3DTR mice.

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

Enzyme-linked immunosorbent assay(ELISA)

… To measure the levels of IgG against CCP2, we used a commercially available ELISA
kit (Euro-Diagnostica, Malmö, Sweden). For the detection of anti-inuenza, and anti-diphtheria toxin
antibodies, 96-well plates were coated with 1 μg/ml of A/H1N1 subunit (Bio-Rad, Hercules, CA, USA) and
diphtheria toxin (List Biological Laboratories, Campbell, CA, USA), respectively. …

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

Materials and methods

Jak2V617F transgenic (TG) mice were generated as previously described [12]. CD11b-diphtheria toxin receptor (DTR) TG mice were purchased from The Jackson Laboratory (Bar Harbor, ME, USA) [13]. These mice were crossed to generate Jak2V617F/CD11b-DTR compound TG. To deplete monocytes in vivo, recipient mice transplanted with BMCs from CD11b-DTR TG mice or Jak2V617F/CD11b-DTR TG mice were administered 15 ng/g diphtheria toxin (DT) (List Biological Labs, Campbell, CA, USA) by intraperitoneal injection every 2 days for 8 weeks. …

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

Key Resources Table
Diphtheria toxin (DT) List Biological Laboratories Cat# 150

In Vivo Cell Depletion

To ablate specific cells, CCR2-DTR, CCR2-DTR, Rag2-/-Il2rg-/-, ROSA26-iDTRMrp8-Cre, BDCA2-DTR, and non-transgenic littermate controls were injected i.p. with 10 ng/g body weight DT on Day -1, Day 0, and Day +2 pi (Espinosa et al., 2017, Swiecki et al., 2010), unless noted otherwise.

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

Depletion and monitoring of platelets

… To model thrombocytopenia by diphtheria toxin receptor-mediated megakaryocyte depletion, PF4-iCre mice were crossed to ROSA26iDTR (iDTR) mice (iDTRPf4). On the first day of depletion, 400 ng of diphtheria toxin (DT) (List Biological Laboratories, Cat. No. 150) was injected i.p. into Cre-positive mice and Cre-negative littermates. On the second and fourth days of depletion, 200 ng of DT was injected i.p. …

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

Antibody selection in microtiter plate

The antibody selection was performed as described previously with modifications33. In brief, for the panning procedure, the antigen was immobilized on a Costar Highbinding 96 well plate (Corning, New York, USA). 1 µg of DT (List Biological Laboratories, Campbell, USA) was diluted in PBS (137 mM NaCl; 1.76 mM KH2PO4 × 2 H2O) and coated in the wells at 4 °C overnight. …

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

Phenotypes of toxin-receptor-gene knockouts by AncBE4max

sgRNAs targeting ANTXR1 and HBEGF were lentiviral infected into HeLa cells. GFP+ cells were FACS sorted and treated with PA/LFnDTA (70 ng/ml PA plus 50 ng/ml LFnDTA) for 48 hours or 7.5 ng/ml Diphtheria toxin (DT, List Biological Laboratories Inc.) for 60 hours and conducted in triplicates with individual treatment. …

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae