Bacterial Toxin Research Citations

STAR★Methods
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DT List Biological Laboratories Cat. #150

Microglia depletion and dissociation

CD11bDTR mice were anesthetized by isoflurane and 1 μL DT solution (20 ng/μl) or saline was injected into the lateral ventricle of the mice. At 12 h after DT-injection, mice were subjected to 3 times FS to test seizure latency and then decapitated for Iba-1 immunostaining, or mice were anesthetized by isoflurane for flow cytometry. …

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

… Diphtheria toxin (in saline; List Biological, no. 150) was injected at a dose of 50μg kg−1 , and the volume was 2.66μl g−1 of body weight as previously described …

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

Intersectional genetic ablation of Tac2 neurons in the spinal cord

Triple mouse line (Tac2Cre, Lbx1flpo and TauDTR mice) was generated for cell ablation. To ablate Tac2 neurons in the spinal cord, i.p. injection of diphtheria toxin (DTX, 40 μg kg−1, List Biological Lab) was injected at day 1 and day 4, respectively41. Behavioral and histochemical experiments were performed 2 weeks after the second DTX injection.

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

Diphtheria Toxin Biological List Laboratories Cat #150

… To induce Cre positive cell deletion, Diphtheria toxin (Biological List laboratories) (100ng/mouse/day) was daily i.p. injected into mice. Cre negative littermate got same amount of DT as control. …

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

Tetramer, antibodies, and other reagents

… For antibody blocking experiments, BC-CML-bearing mice were injected with 200 μg/mouse αPD-1 (RMP1-14, BioLegend), 200 μg/mice αLAG3 (C9B7W;64 gift of Dario Vignali and Creg Workman), 200 μg/mice αTim-3 (RMT3-23, BioLegend), 500 μg/mice αTIGIT (10A7, Genentech) or respective murine or rat isotype controls (BioXCell), i.p. three times per week for 2 weeks starting from day 7 post BMT. Diphtheria toxin (DT) was from List Biological Laboratories. …

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

… For basophil depletion, WT and basophil-depleted mice were treated with 0.375μg of diphtheria toxin (catalog no. 150; LIST Biological Laboratories) intraperitoneally every other day; mice were killed 37?days post-N. brasiliensis infection …

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

Diphtheria-Tetanus-Acellular Pertussis Multiplex Immunoassay

Total IgG concentrations against vaccine antigens pertussis toxin (PT), pertactin (PRN), filamentous hemagglutinin (FHA), fimbriae 2/3 (FIM 2/3), tetanus toxin (TT), and diphtheria toxoid (DT) were measured using an in-house multiplex bead-based immunoassay. PT, PRN, and FHA were kindly provided by GlaxoSmithKline (Belgium). TT was purchased from Sigma-Aldrich while DT and FIM 2/3 was sourced from List biological laboratories (USA). A standard curve was generated using a 10-step 3-fold serial dilution of an in-house reference sera previously quantified against National Institute for Biological Standards and Control reference sera: PT (06/140), TT (TE-3), and DT (10/262). The concentration of FIM 2/3 IgG was previously assigned to 06/140 by an international collaborative study (22). Blanks and two QC samples were included on every plate to calculate% critical variance across all assays, which fell between 6 and 12.7%. Assay specificity was determined using inhibition and interference assays. No cross reactivity was detected (data not shown).

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae
• Product #186 – Fimbriae 2/3 from B. pertussis

… To deplete inflammatory monocytes, CCR2-DTR mice or their cohoused control littermates were injected intraperitoneally with 200 ng (10 ng/g body weight) of DT (List Biological Laboratories) as noted for a total of four doses …

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

… To deplete CCR2+ monocytes, 5g per kg DT (List Biological Laboratories) was injected into all mice at day 10, 13, 16 and 19 …

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

SM22α-hDTR mice

All animal studies were undertaken under UK Home Office licensing and approved by the Animal Welfare Ethical Review Body of the University of Cambridge. C57BL6/J SM22α-hDTR mice were generated and genotyped as previously described [13]. Male and female SM22α-hDTR mice and wild type littermates were treated as indicated with intraperitoneal doses of 5 ng/g body weight diphtheria toxin (LIST biological laboratories, 150) diluted in 0.9% saline and 0.2% BSA.

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae