Bacterial Toxin Research Citations

… Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae list labs Cat# 150 …

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

Animals and deafening protocol

… Diptheria toxin (DT; List Biologicals Lab. Inc. cat. #150) was dissolved in saline, aliquoted and stored at − 20 °C until use. Repeat freeze–thaw cycles were avoided. A single dose of 20 ng/g DT was administered intra-muscularly to adult mice. Mice receiving adenovirus (Ad) inoculation surgery, were given DT immediately after the surgery. High-calorie diet gel (DietGel 76A, ClearH20) and an extra bottle of water were provided for the 2 following weeks. Body weight and general health metrics were monitored every day for a week after DT administration.

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

… For Treg depletion, diphtheria toxin (DT; List Biologicals, Campbell, CA) was administered intraperitoneally 2 days and 1 day before intratracheal instillation of bacteria and then on days 1, 3 after inoculation. …

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

Reagent:

Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae List Biological Laboratories Cat# 150

Tumor Associated Macrophage Co-Injection Experiments

Foxp3DTR-GFP donor mice were injected as mentioned above with either PyMT or EO771 cells, and their tumors were allowed to grow until approximately 90mm3. Half of the donors received two doses of 50 μg/kg of diphtheria toxin (DT) in two consecutive days, and primary tumors from both groups were harvested four days after initial DT treatment.

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

… (2) DTR mice with DT injection at 5 weeks of age (DT group, N=32 animals). Mice in both groups received a single intramuscular injection of DT (15 or 20 ng/g) The DT (List Biological Laboratories, Inc., Campbell CA. Product #150) was reconstituted to a concentration of 2 mg/ml, aliquoted, and stored at 20°C. The aliquots were thawed and diluted in saline 1:200 just prior to the injection …

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

Mice and reagents.

C57BL/6 (B6), B6 CD45.1, BALB/c, CBA, B6 Foxp3-DTR, and B6 Foxp3-IRES-GFP mice were purchased from The Jackson Laboratory. As indicated, select lung and heart transplant recipients were treated with perioperative costimulatory blockade consisting of anti-CD40 ligand (250 μg intraperitoneally [i.p.], day 0) and CTLA-4–Ig (200 μg i.p., day 2) (Bio X Cell). Diphtheria toxin (DT) (List Biological Laboratories Inc.) (500 ng i.p. days 0, 1, and 7) was given to select B6 Foxp3-DTR heart transplant recipients to deplete Foxp3+ cells. …

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

Animal Models

… For myeloid-depletion experiments, 250 ng DT (List Biological Labs, Campbell, CA) was injected intraperitoneally every other day for 4 days after DSS initiation, …

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

2.3. Drug Administration

Diphtheria toxin (List Biological Laboratories, Campbell, CA, USA), with a half-life of ~18 h, was dissolved in 0.9% saline and administered intramuscularly [13]. …

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

STAR★Methods
Key Resources Table

Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae List Biological Laboratories Cat# 150

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

STAR★Methods

… Diphteria Toxin (DTX) List Biological Lab Cat# 150 …

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae