Bacterial Toxin Research Citations

CD115+ M-MDSC depletion

MMDTR mice were implanted with 2.5 × 105 EL4 cells. Each mouse received one intraperitoneal injection (10 ng/g of body weight) of diphtheria toxin (DT) (List Biological Laboratories, Campbell, CA, USA) on days 7, 9, and 11 after tumor implantation (Supplementary Fig. 7b). Time intervals (every 48 h) of DT injection were optimized by flow cytometry blood analysis (Supplementary Fig. 7a). After sacrifice on day 14 after tumor implantation, single cells were prepared for flow cytometry analysis. CD11b+Ly6G+Ly6Clo cells from naive or TB mice were sorted for OLFM4 immunohistochemistry (IHC) and qRT-PCR analysis. C57BL/6 mice were used as a negative control to exclude effects related to DT.

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

Mouse techniques

… For diphtheria toxin receptor (DTR) ablation studies, WT and indicated DTR mice received injection twice intratracheally (i.t.) with 100 ng diphtheria toxin (DT) in 20 μl of sterile PBS or i.p. with 200 ng DT in 100 μl of sterile PBS (150, List Biological Lab) on 1 d before and after metastatic inoculation. Cell of interest depletion was confirmed by flow cytometry. …

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

Tregs depletion and agent administration

To deplete Tregs, DEREG mice were injected intraperitoneally with 1 μg DT (List Biological Laboratories, inc, USA) 24 h before LPC injection, then 0.5 μg DT every third day until 10 days after LPC injection. Control mice were treated with PBS injection. Flow cytometry in spleens and blood were used to confirm Tregs depletion. …

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

… Diphtheria toxin, Unnicked, from Corynebacterium diphtheriae List Biological Laboratories Cat# 150 …

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

… Where indicated, Foxp3+ Tregs were ablated in host mice via ip administration of diphtheria toxin (List Biologicals) at 50 mg/kg on the day of adoptive transfer …

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

… BDCA2-DTR mice. After 6 weeks for reconstitution, mice were then depleted of pDCs by intraperitoneal injection of Diphtheria toxin (DT- List Biological Laboratories) every 48 hours for up to 2 weeks …

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

… To induce hair cell damage, DT (List Biological Laboratories, Inc., Campbell, CA) at 25 or 50 ng/g was injected intramuscularly at 7 weeks of age (1-week post-tamoxifen) once a day on two days spaced 44-48 hours apart. We selected a DT dose for each experiment based on periodic tests of DT potency, which varied with each lot and diminished over time during storage at -20°C …

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

… Mgl2-DTR mice were injected intraperitoneally with 1 µg of DT (List Biological Laboratories) 28 days after primary infection (loading dose). Maintenance dosing of 100 ng …

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

… For Tregs depletion, Foxp3-DTR mice were injected intraperitoneally with 1 µg DT (List Biological Laboratories, Inc, USA) 24 h before BCAS, then 0.5 µg every three days …

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

Mice treatment

CD169-DTR heterozygous or wild-type (WT) male mice were intraperitoneally injected with 40 ng/g of diphtheria toxin (DT) (List Labs, Campbell, CA, USA) diluted in 200 μl DPBS at early stages (a day before and 4 days after tumor injection) and intermediate stages (5 and 10 days after tumor injection) of tumor progression. …

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae