Bacterial Toxin Research Citations

2.14. Cell depletion in mice

Diphtheria toxin receptor (DTR)-transgenic mice were intraperitoneally administered 40 μg/kg of diphtheria toxin (DT) (List Labs, Campbell, CA, USA) to deplete plasmacytoid dendritic cells (pDCs) in BDCA2-DTR mice or CD169+ cells in CD169-DTR mice. To deplete lung macrophages, mice were intranasally (100 μl/mouse) and intravenously (200 μl/mouse) treated with clodronate liposomes (Liposoma BV, Amsterdam, Netherlands).

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

… Depletion of AMs was induced by intranasal instillation of 15ng/mouse of diphtheria toxin (DT, List Biological Laboratories) with double administration three days apart …

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

Diphtheria toxin, VEGF, PD-1, CCR8 antibody and CCR2i treatments

DT (List Biological Laboratories) was administered to mice (1 μg per mouse in PBS) via retro-orbital injection twice on two consecutive days. For tumor transplantation experiments, DT was injected on days 8 and 9 after tumor s.c. transplantation. Mouse polyclonal neutralizing VEGF-A antibody (R&D clone AF-493-M) or control IgG (BioXcell clone BE0130) were injected on days 9, 12 and 15 (20 μg per mouse per injection) with or without DT, or on days 8, 10, 12, 14 and 17 with or without CCR8 antibody (BioLegend clone SA214G2; 240 μg per mouse per injection). …

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

… For cell-type specific ablations, diphtheria toxin (30 ng/g of mouse; List Biological Laboratories, Inc.) was injected intraperitoneally at P12 for PC-X and PC-eX animals …

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

Diphtheria toxin (DT) administration

Diphtheria toxin (DT) powder was purchased from List Biological Laboratories (Campbell, CA, USA) and dissolved in 0.9% sterile saline at 2 mg/ml of stock concentration and stored at −20◦C.
Stock DT solution was further diluted into 1 µg/ml of working concentration before use. Neonatal mice received a single dose of either DT injection at the dosage of 5 ng/g body weight or 0.9% saline injection at P5 or P14 (Tong et al., 2015). The injection was made in the thigh muscle of the right hind leg. Following either saline or DT injection, mice were allowed to survive to the age of P27–35 before tissue collection.

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

… Diphtheria toxin (DT) List Biological Laboratories Cat#150 …

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

… CD11c-DTR MRL/lpr mice and transgene-negative littermate controls were i.p. injected with a single dose of DT (List Biological Labs) at a concentration of 8 ng/kg body weight in PBS at 1, 4, or 7 d prior to experimental analysis …

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

… Neuronal ablation Mice carrying the Hes2iCre;hCdx2::FlpO alleles in addition to the effector Tauds-DTR received i.p. injections of diphtheria toxin (DTX, 50 ng/gram of weight; List Biological Laboratories) at P28 and P31, or saline solution in equal volume as control. …

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

… After 30 days, these animals were administered diphtheria toxin (DT, 50 ng/g BW, List Biological Lab, California) for three continuous days to deplete microglia (Parkhurst et al., 2013). …

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

In vivo treatments with chemicals and antibodies

For DT-induced depletion of IM, IMDTR mice were injected i.p. with a single dose of 50 ng DT (List Biological Labs, 150), unless otherwise stated. …

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae